Effects of syringe material and temperature and duration of storage on the stability of equine arterial blood gas variables

Deane, Joanne C; Dagleish, Mark P.; Benamou, A. E.; Wolf, B. T.; Marlin, D. J.

doi:10.1111/j.1467-2995.2004.00173.x

Cited by 25 publications

(33 citation statements)

References 16 publications

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“…Changes in the concentration of NMR observable metabolites through freezing and thawing are controversial, with both a statistically significant decrease in glucose, cholesterol and volatile fatty acids and an increase in lactate reported by one research team (Laborde et al 1995), but no influence found by another (Morris et al 2002). Repetitive freezing and thawing of plasma has also been reported to decrease the concentration of urea-N (Deane et al 2004). In our dataset, however, urea did not show any significant correlation to storage time (r = -0.06).…”

Section: Discussionmentioning

confidence: 99%

NMR-based metabonomics of bovine blood: an investigation into the effects of long term storage on plasma samples

2013

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Section: Discussionmentioning

confidence: 99%

NMR-based metabonomics of bovine blood: an investigation into the effects of long term storage on plasma samples

2013

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“…The estimated linear slope for the PO 2 ‐time relationship of equine blood at 22°C in the glass syringes used in the study reported here (−0.042 mmHg/min) were much lower than that reported for human blood kept at room temperature (−0.84 to −1.37 mmHg/min; Pretto and Rochford 1994; Smeenk et al . 1997) or horse blood kept at room temperature (−0.11 mmHg/min; Deane et al . 2004).…”

Section: Discussionmentioning

confidence: 99%

Effects of collecting blood into plastic heparinised Vacutainer^®tubes and storage conditions on blood gas analysis values in horses

Noel¹,

Couëtil

Constable

2010

Equine Veterinary Journal

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SummaryReasons for performing study: Plastic heparinised Vacutainer â (registered trademark of Becton, Dickinson and Company) tubes are used for blood gas analysis in horses. This collection method may not be ideal because influx of atmospheric O 2 through the permeable plastic wall of the Vacutainer â tube and loss of CO 2 into the gas phase above the blood sample should increase blood PO 2 and decrease PCO 2 , respectively.Objectives: To determine the effects of collecting blood into plastic Vacutainer â tubes and storage conditions on blood gas analysis values.Methods: Blood was obtained from 6 healthy horses and tonometered at 37°C with 12% O 2 and 5% CO 2 . Three ml aliquots of tonometered blood were collected using a glass syringe or Vacutainer â tube and stored in iced water or at room temperature for 0,

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“…In front of the difficulties in the practical clinics and the limitations that can be placed against that, is indispensable to store the blood on ice. Although, there is a lack of consensus in literature regarding the amount of time blood samples can be feasible and if that storage is really effective 3,4,6,7,9,10 . The samples were collected at the same moment and analyzed in different times: 20 minutes, 35 minutes and 50 minutes.…”

Section: Discussionmentioning

confidence: 99%

“…The possibility of blood samples maintenance at low temperatures is an object of interest in some scientific researches which make use of bovine 2,4,5,6 , equine 5,7,8 , canine 9 and porcine 10 species. However, there is a lack of consensus in literature regarding the maximum amount of time blood samples can be stored for blood gas analysis at low temperatures.…”

Section: Introductionmentioning

confidence: 99%

Effects of blood storage on ice in biochemical and arterial blood gas analysis of rats

et al. 2008

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Purpose:To investigate the effects of blood storage in biochemical and arterial blood gas analysis of Wistar rats. Methods: Ten adult male rats with weights between 300-350 g were used. The catheterization of the internal carotid artery were performed, followed by withdrawal of 3 ml of blood using 3 separate syringes each containing 1 ml. The syringes were fulfilled one after another and numerated according to the order of collection. Then, the following groups were devised: GT1 -samples that were firstly analyzed in 20 minutes and the second exam 65 minutes after the collection; GT2 -samples that were firstly analyzed in 35 minutes and the second exam 80 minutes after the collection; GT3 -samples that were firstly analyzed in 50 minutes and the second exam 95 minutes after the collection. The samples were stored in a container with a temperature between 0 Cº and 4 Cº. Upon analysis the values of pH, PaCO 2 , PaO 2 , HCO 3 -, SatO 2 , SBE, Na + and K + concentrations and glucose were compared. Results: No statistically relevant difference (p<0.05) between the GT1, GT2 or GT3, when measuring pH, PaCO 2 , PaO 2 , HCO 3 -, SatO 2 , SBE or glucose, were found. However, Na + concentration decreased and the K + concentration increased (p<0.05) when comparing the first analysis (20 minutes) with the last one (95 minutes). Conclusion:The blood storage on ice does not interfere in the results of arterial blood gas analysis for the period of at least 95 minutes, except for the Na + e K + levels, which are practicable until 80 minutes after storage. Key words: Blood Preservation. Blood Gas Analysis. Rats. RESUMO Objetivo:Analisar o efeito da estocagem sanguínea na bioquímica e na gasometria do sangue arterial de ratos Wistar. Métodos: Foram utilizados 10 ratos adultos, machos, com peso compreendido entre 300 a 350 gramas. Cada animal foi submetido à cateterização da artéria carótida seguida de heparinização prévia do animal. Foram colhidos 3 ml de sangue total do rato, separados em três seringas contendo 1 ml cada. As seringas foram preenchidas uma após a outra e enumeradas respectivamente à ordem de coleta. De acordo com esta numeração as amostras foram distribuídas nos seguintes grupos: GT1 -amostras que tiveram primeira análise em 20 minutos e a segunda análise 65 minutos após a coleta; GT2 -amostras que tiveram primeira análise em 35 minutos e segunda análise 80 minutos após a coleta; GT3 -amostras que tiveram primeira análise em 50 minutos e 95 minutos após a coleta. As amostras foram estocadas em material isolante térmico, com temperatura entre 0 Cº e 4 Cº.

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Effects of syringe material and temperature and duration of storage on the stability of equine arterial blood gas variables

Cited by 25 publications

References 16 publications

NMR-based metabonomics of bovine blood: an investigation into the effects of long term storage on plasma samples

NMR-based metabonomics of bovine blood: an investigation into the effects of long term storage on plasma samples

Effects of collecting blood into plastic heparinised Vacutainer^®tubes and storage conditions on blood gas analysis values in horses

Effects of blood storage on ice in biochemical and arterial blood gas analysis of rats

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Effects of syringe material and temperature and duration of storage on the stability of equine arterial blood gas variables

Cited by 25 publications

References 16 publications

NMR-based metabonomics of bovine blood: an investigation into the effects of long term storage on plasma samples

NMR-based metabonomics of bovine blood: an investigation into the effects of long term storage on plasma samples

Effects of collecting blood into plastic heparinised Vacutainer®tubes and storage conditions on blood gas analysis values in horses

Effects of blood storage on ice in biochemical and arterial blood gas analysis of rats

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Effects of collecting blood into plastic heparinised Vacutainer^®tubes and storage conditions on blood gas analysis values in horses