Purpose:To investigate the effects of blood storage in biochemical and arterial blood gas analysis of Wistar rats. Methods: Ten adult male rats with weights between 300-350 g were used. The catheterization of the internal carotid artery were performed, followed by withdrawal of 3 ml of blood using 3 separate syringes each containing 1 ml. The syringes were fulfilled one after another and numerated according to the order of collection. Then, the following groups were devised: GT1 -samples that were firstly analyzed in 20 minutes and the second exam 65 minutes after the collection; GT2 -samples that were firstly analyzed in 35 minutes and the second exam 80 minutes after the collection; GT3 -samples that were firstly analyzed in 50 minutes and the second exam 95 minutes after the collection. The samples were stored in a container with a temperature between 0 Cº and 4 Cº. Upon analysis the values of pH, PaCO 2 , PaO 2 , HCO 3 -, SatO 2 , SBE, Na + and K + concentrations and glucose were compared. Results: No statistically relevant difference (p<0.05) between the GT1, GT2 or GT3, when measuring pH, PaCO 2 , PaO 2 , HCO 3 -, SatO 2 , SBE or glucose, were found. However, Na + concentration decreased and the K + concentration increased (p<0.05) when comparing the first analysis (20 minutes) with the last one (95 minutes).
Conclusion:The blood storage on ice does not interfere in the results of arterial blood gas analysis for the period of at least 95 minutes, except for the Na + e K + levels, which are practicable until 80 minutes after storage. Key words: Blood Preservation. Blood Gas Analysis. Rats.
RESUMO
Objetivo:Analisar o efeito da estocagem sanguínea na bioquímica e na gasometria do sangue arterial de ratos Wistar. Métodos: Foram utilizados 10 ratos adultos, machos, com peso compreendido entre 300 a 350 gramas. Cada animal foi submetido à cateterização da artéria carótida seguida de heparinização prévia do animal. Foram colhidos 3 ml de sangue total do rato, separados em três seringas contendo 1 ml cada. As seringas foram preenchidas uma após a outra e enumeradas respectivamente à ordem de coleta. De acordo com esta numeração as amostras foram distribuídas nos seguintes grupos: GT1 -amostras que tiveram primeira análise em 20 minutos e a segunda análise 65 minutos após a coleta; GT2 -amostras que tiveram primeira análise em 35 minutos e segunda análise 80 minutos após a coleta; GT3 -amostras que tiveram primeira análise em 50 minutos e 95 minutos após a coleta. As amostras foram estocadas em material isolante térmico, com temperatura entre 0 Cº e 4 Cº.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.