Effects of Synergistic Inhibition on &amp;alpha;-Glucosidase by Phytoalexins in Soybeans

Son, Hyeong-U; Yoon, Eun-Kyeong; Yoo, Chi-Yeol; Park, Chul‐Hong; Bae, Myung‐Ae; Kim, Sang Woo; Lee, Chang Hyung; Lee, Ki-Won; Seo, Hogyun; Kim, Kyung-Jin; Lee, Sang‐Han

doi:10.20944/preprints201910.0223.v1

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2019

DOI: 10.20944/preprints201910.0223.v1

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Effects of Synergistic Inhibition on α-Glucosidase by Phytoalexins in Soybeans

Hyeong-U Son

Eun-Kyeong Yoon

Chi-Yeol Yoo

et al.

Abstract: To determine the mode of action of the effects of phytoalexins in soybeans, we analyzed enzyme inhibition kinetics using Michaelis–Menten plots and the Lineweaver–Burk plots. The results showed that glyceollin showed competitive inhibition, genistein showed noncompetitive, daidzein was uncompetitive, and luteolin showed a mixed mode of action. The Ki values were determined using a Dixon plot as: glyceollin, 18.99; genistein, 15.42; luteolin, 16.81; and daidzein, 9.99 μM, respectively… Show more

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Cited by 5 publications

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Biomolecules from Different Angles

Uversky

2020

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Biomolecules from Different Angles

Uversky

2020

Biomolecules

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Polyphenolic Compounds and Biological Activities of Leaves and Fruits of Syzygium samarangense cv. ‘Giant Green’ at Three Different Maturities

et al. 2023

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Syzygium samarangense cv. ‘Giant Green’ is an underutilised fruit that can be found in Malaysia and other Asian countries. Since this fruit is not fully commercialised, the information about its potential health benefits is limited. Thus, this study was carried out to determine the polyphenolic contents (total phenolic and total flavonoid) and biological activities (antioxidant, alpha-glucosidase and antibacterial assay) of ‘Giant Green’ leaves and fruits at different maturity stages. The young, mature and old leaves, and unripe, half-ripened and ripened fruits were analysed. The results showed that the young leaves increased the TPC and TFC by 35% and 41%, over the old leaves. Similarly, TPC and TFC contents were 37% and 54% higher in unripe fruits compared to the ripened fruits. In addition, young leaves exhibited the strongest scavenging activity towards DPPH, NO and ABTS radicals with IC50 values increasing 1.6-fold, 1.7-fold and 2.3-fold, respectively, over the old leaves. However, in fruit samples, only unripe fruits were able to inhibit more than 50% of radicals. A comparable trend was observed in alpha-glucosidase inhibitory assay whereas young leaves and unripe fruits recorded 81% and 99% increases in IC50 values, respectively, from young leaves to old leaves and unripe fruits to ripened fruits. Identically, young leaves also showed a significant effect in antibacterial assay with an inhibition zone increase of 19%, 36%, 32%, and 31% in S. aureus, E. faecalis, S. typhimurium and E. coli, respectively, over the old leaves. However, only unripe fruits were most effective against all tested bacteria while half-ripened fruits were only effective against E. faecalis with a 1.1-fold increase in the inhibition zone compared to unripe fruits. Ripened fruits were resistant to all of the bacteria. These results suggest that the young leaves and unripe fruits of ‘Giant Green’ cultivar of S. samarangense could be a potential candidate for the management of some diseases coming from harmful free radicals or bacterial infection.

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Phytochemicals with Chemopreventive Activity Obtained from the Thai Medicinal Plant Mammea siamensis (Miq.) T. Anders.: Isolation and Structure Determination of New Prenylcoumarins with Inhibitory Activity against Aromatase

Luo

Manse

Chaipech

et al. 2022

IJMS

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With the aim of searching for phytochemicals with aromatase inhibitory activity, five new prenylcoumarins, mammeasins K (1), L (2), M (3), N (4), and O (5), were isolated from the methanolic extract of Mammea siamensis (Miq.) T. Anders. flowers (fam. Calophyllaceae), originating in Thailand. The stereostructures of 1–5 were elucidated based on their spectroscopic properties. Among the new compounds, 1 (IC50 = 7.6 µM) and 5 (9.1 µM) possessed relatively strong inhibitory activity against aromatase, which is a target of drugs already used in clinical practice for the treatment and prevention of estrogen-dependent breast cancer. The analysis through Lineweaver–Burk plots showed that they competitively inhibit aromatase (1, Ki = 3.4 µM and 5, 2.3 µM). Additionally, the most potent coumarin constituent, mammea B/AB cyclo D (31, Ki = 0.84 µM), had a competitive inhibitory activity equivalent to that of aminoglutethimide (0.84 µM), an aromatase inhibitor used in therapeutics.

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Effects of Synergistic Inhibition on α-Glucosidase by Phytoalexins in Soybeans

Cited by 5 publications

References 18 publications

Biomolecules from Different Angles

Biomolecules from Different Angles

Polyphenolic Compounds and Biological Activities of Leaves and Fruits of Syzygium samarangense cv. ‘Giant Green’ at Three Different Maturities

Phytochemicals with Chemopreventive Activity Obtained from the Thai Medicinal Plant Mammea siamensis (Miq.) T. Anders.: Isolation and Structure Determination of New Prenylcoumarins with Inhibitory Activity against Aromatase

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