Effects of Surface Topography, Hydrophilicity and Chemistry of Surface-treated PCL Scaffolds on Chondrocyte Infiltration and ECM Production

Janvikul, Wanida; Uppanan, Paweena; Thavornyutikarn, Boonlom; Kosorn, Wasana; Kaewkong, Pakkanun

doi:10.1016/j.proeng.2013.05.106

Cited by 33 publications

(20 citation statements)

References 7 publications

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“…To this end, biomimetic surface coatings and modifications using appropriate durable and biocompatible nanomaterials have already been applied [ 4 – 7 ]. To date, various sophisticated tissue-engineering structures that mimic the extracellular matrix (ECM) have been proposed, which aim to induce the highly desirable in situ endothelialization of vascular biomaterials while minimizing thrombogenicity and inflammation [ 8 – 10 ]. In the case of cardiovascular implants, though, the successful revascularization after implantation remains an unmet clinical problem [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Oxygen-plasma-modified biomimetic nanofibrous scaffolds for enhanced compatibility of cardiovascular implants

Pappa¹,

Karagkiozaki²,

Krol³

et al. 2015

Beilstein J. Nanotechnol.

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SummaryElectrospun nanofibrous scaffolds have been extensively used in several biomedical applications for tissue engineering due to their morphological resemblance to the extracellular matrix (ECM). Especially, there is a need for the cardiovascular implants to exhibit a nanostructured surface that mimics the native endothelium in order to promote endothelialization and to reduce the complications of thrombosis and implant failure. Thus, we herein fabricated poly-ε-caprolactone (PCL) electrospun nanofibrous scaffolds, to serve as coatings for cardiovascular implants and guide tissue regeneration. Oxygen plasma treatment was applied in order to modify the surface chemistry of the scaffold and its effect on cell attachment and growth was evaluated. The conditions of the surface modification were properly adjusted in order to define those conditions of the treatment that result in surfaces favorable for cell growth, while maintaining morphological integrity and mechanical behavior. Goniometry (contact angle measurements), scanning electron microscopy (SEM), atomic force microscopy (AFM), and X-ray photoelectron spectroscopy (XPS) measurements were used to evaluate the morphological and chemical changes induced by the plasma treatment. Moreover, depth-sensing nanoindentation was performed to study the resistance of the plasma-treated scaffolds to plastic deformation. Lastly, the cell studies indicated that all scaffolds were cytocompatible, with the plasma-treated ones expressing a more pronounced cell viability and adhesion. All the above findings demonstrate the great potential of these biomimetic tissue-engineering constructs as efficient coatings for enhanced compatibility of cardiovascular implants.

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Section: Introductionmentioning

confidence: 99%

Oxygen-plasma-modified biomimetic nanofibrous scaffolds for enhanced compatibility of cardiovascular implants

Pappa¹,

Karagkiozaki²,

Krol³

et al. 2015

Beilstein J. Nanotechnol.

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“…It is likely that adsorption and immobilization are both occurring at the scaffold surface as XPS analysis of the scaffold in the absence of conjugation reagents (Table ) showed that a measure of surface adsorption (6.7%) was detected for the H 2 O plasma activated scaffold. This was not unexpected as water plasma activation decreases the hydrophobicity of PCL, increasing protein adoption (Janvikul, Uppanan, Thavornyutikarn, Kosorn, & Kaewkong, ). This localization of C x H y N z + secondary ions suggests that a nitrogen‐containing film is attached to the scaffold.…”

Section: Discussionmentioning

confidence: 92%

Time‐of‐flight secondary ion mass spectrometry three‐dimensional imaging of surface modifications in poly(caprolactone) scaffold pores

Taylor

Graham

Gamble

2019

J Biomedical Materials Res

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Scaffolds composed of synthetic polymers such as poly(caprolactone) (PCL) are widely used for the support and repair of tissues in biomedicine. Pores are common features in scaffolds as they facilitate cell penetration. Various surface modifications can be performed to promote key biological responses to these scaffolds. However, verifying the chemistry of these materials post surface modification is problematic due to the combination of three-dimensional (3D) topography and surface sensitivity.Time-of-flight secondary ion mass spectrometry (ToF-SIMS) is commonly used to correlate surface chemistry with cell response. In this study, 3D imaging mass spectrometry analysis of surface modified synthetic polymer scaffolds is demonstrated using PCL porous scaffold, a pore filling polymer sample preparation, and 3D imaging ToF-SIMS. We apply a simple sample preparation procedure, filling the scaffold pores with a poly(vinyl alcohol)/glycerol mixture to remove topographic influence on image quality. This filling method allows the scaffold (PCL) and filler secondary ions to be reconstructed into a 3D chemical image of the pore. Furthermore, we show that surface modifications in the pores of synthetic polymer scaffolds can be mapped in 3D.Imaging of "dry" and "wet" surface modifications is demonstrated as well as a comparison of surface modifications with relatively strong ToF-SIMS peaks (fluorocarbon films [FC]) and to more biologically relevant surface modification of a protein (bovine serum albumin [BSA]). We demonstrate that surface modifications can be imaged in 3D showing that characteristic secondary ions associated with FC and BSA are associated with C 3 F 8 plasma treatment and BSA, respectively within the pore. K E Y W O R D S 3D, imaging, scaffold, surface modifications, ToF-SIMS, XPS

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“…Stem cells ability to proliferate in damaged tissue is highly important feature in tissue engineering applications. Diverse environmental conditions, scaffold chemical composition and topography have a significant influence to cell proliferation processes. Moreover, most of the literature analyses the effect of micro‐ and nano‐structures, while there is just a little of information about macro‐structured scaffold impact on cell growth.…”

Section: Discussionmentioning

confidence: 99%

The effect of larger than cell diameter polylactic acid surface patterns on osteogenic differentiation of rat dental pulp stem cells

Alksnė

Šimoliūnas

Kalvaitytė

et al. 2018

J Biomedical Materials Res

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Topography of the scaffold is one of the most important factors defining the quality of artificial bone. However, the production of precise micro-and nano-structured scaffolds, which is known to enhance osteogenic differentiation, is expensive and time-consuming. Meanwhile, little is known about macro-patterns (larger than cell diameter) effect on cell fate, while this kind of structures would significantly facilitate the manufacturing of artificial skeleton. Therefore, this research is focused on polylactic acid scaffold's macropattern impact on rat's dental pulp stem cells (DPSCs) morphology, proliferation, and osteogenic differentiation. For this study, two types of scaffolds were 3D printed: wavy and porous. Wavy scaffolds consisted of 188 μm wide joined threads, meaning that cells might have been curved on the filament as well as compressed in the groove. Porous scaffolds were designed to avoid groove formation and consisted of 500 μm threads, arranged in the woodpile manner, forming 300 μm diameter pores. We found that both macro-surfaces influenced DPSC morphology compared to control. As a consequence, enhanced DPSC proliferation and increased osteogenic differentiation potential was registered in cells grown on these scaffolds. Finally, our results showed that the construction of an artificial bone did not necessarily require the precise structuring of the scaffold, because both types of macrotopographic PLA scaffolds were sufficient enough to induce spontaneous DPSC osteogenic differentiation. How to cite this article: Alksne M, Simoliunas E, Kalvaityte M, Skliutas E, Rinkunaite I, Gendviliene I, Baltriukiene D, Rutkunas V, Bukelskiene V. 2019. The effect of larger than cell diameter polylactic acid surface patterns on osteogenic differentiation of rat dental pulp stem cells. J Biomed Mater Res Part A 2019:107A:174-186.

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Effects of Surface Topography, Hydrophilicity and Chemistry of Surface-treated PCL Scaffolds on Chondrocyte Infiltration and ECM Production

Cited by 33 publications

References 7 publications

Oxygen-plasma-modified biomimetic nanofibrous scaffolds for enhanced compatibility of cardiovascular implants

Oxygen-plasma-modified biomimetic nanofibrous scaffolds for enhanced compatibility of cardiovascular implants

Time‐of‐flight secondary ion mass spectrometry three‐dimensional imaging of surface modifications in poly(caprolactone) scaffold pores

The effect of larger than cell diameter polylactic acid surface patterns on osteogenic differentiation of rat dental pulp stem cells

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