Effects of surface roughness and dimorphism on the adhesion of <scp><i>Candida albicans</i></scp> to the surface of resins: scanning electron microscope analyses of mode and number of adhesions

Mayahara, Mitsuori; Kataoka, Ryuta; Arimoto, Takafumi; Tamaki, Yukimichi; Yamaguchi, Nobuaki; Watanabe, Yūki; Yamasaki, Yoshizumi; Miyazaki, Takashi

doi:10.1111/jicd.12055

Cited by 42 publications

(39 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Increased surface roughness can facilitate bacterial accumulation, which then promotes development of denture stomatitis, caries, and periodontal disease 8,9,30) . In addition, it was reported that the morphological transformation between fungal blastospores and hyphae in C. albicans biofilm coincides with an increase in surface roughness of denture acrylic resin 31,32) , a phenomenon attributed to thigmotropism (contact sensing) by C. albicans, in which yeast cells switch to hyphal growth upon contact with a surface 33) . Invasion of hyphae via a few cracks in resin induces hyphal growth and formation of biofilm 34) , thus increasing pathogenicity and virulence.…”

Section: Discussionmentioning

confidence: 99%

Development of antibacterial denture cleaner for brushing containing tea tree and lemongrass essential oils

Koseki

Tanaka

Murata

2018

Dental Materials Journal

View full text Add to dashboard Cite

We evaluated effectiveness of tea tree oil (TO) and lemongrass oil (LO) for removal of Candida biofilm from denture base resin and their influence on that surface. Biofilm of C. albicans was formed on resins, and immersed in various concentrations of each oil and distilled water (DW). The biofilm removal effect was determined by incubating specimens in RPMI medium containing Alamar blue (AB) and measuring absorbance. Wear test was also conducted, and surface condition of resins was determined using laser scanning microscope and digital microscope. Specimens immersed in the TO and LO solutions tended to have a lower AB value at higher concentrations and longer soaking times. Use of these agents resulted in less surface roughness as compared to DW. Our results suggest that TO and LO were valid to remove biofilm attached to resin with lower levels of abrasion, and these are effective for use in denture cleaner.

show abstract

Section: Discussionmentioning

confidence: 99%

Development of antibacterial denture cleaner for brushing containing tea tree and lemongrass essential oils

Koseki

Tanaka

Murata

2018

Dental Materials Journal

View full text Add to dashboard Cite

show abstract

“…As in previous investigations on the adherence and biofilm formation of Candida albicans to denture‐base resins, the arithmetic Ra was chosen to represent the surface roughness in the present study …”

Section: Discussionmentioning

confidence: 99%

“…While some studies have shown that, during initial adherence, C. albicans is retained in greater amounts on rough surfaces in comparison to smooth acrylic surfaces, others have observed no significant influence of surface roughness on the initial attachment of C. albicans . It is important to note, however, that although in these studies the substrates were prepared to produce different surface morphologies, only a few investigated the adherence of C. albicans to denture‐base resins over wider range of roughness . Moreover, to date, only a few studies have reported on the effect of surface roughness on C. albicans biofilm formation (mature biofilm) on denture‐base resins .…”

Section: Introductionmentioning

confidence: 99%

Effect of surface roughness on the hydrophobicity of a denture‐base acrylic resin andCandida albicanscolonization

Foggi

Machado

Zamperini

et al. 2014

J of Invest & Clin Dent

View full text Add to dashboard Cite

Aim: The aim of the present study was to evaluate the effect of surface roughness (roughness average [Ra] lm) on the hydrophobicity of a denture-base acrylic resin and the initial adherence and biofilm formation of Candida albicans (C. albicans). Methods: Disk-shaped specimens were divided into six groups: Ra 0.05, Ra 0.2, Ra 0.4, Ra 0.8, Ra 1.5, and Ra 3.0. Water contact angles (WCA) were measured, and the specimens incubated with C. albicans for 90 min (initial adherence, n = 108) or 48 h (biofilm formation, n = 108). Adhered and biofilm cells were evaluated by c.f.u./mL and 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT), and the correlation between the two methods was evaluated. The surface of the specimens and cells (adhered and biofilm) were also analyzed by scanning electron microscopy (SEM). Results: Groups Ra 0.05 and 3.0 exhibited the lowest (~75°) and the highest (~100°) WCA mean values, respectively. For both initial adherence and biofilm formation, no statistically-significant differences were observed among all groups, as determined by c.f.u./mL and XTT. A positive correlation between these two methods was found. SEM analysis showed the presence of scratches and valleys on the acrylic specimens and densely-packed yeast cells covering the entire surface. Conclusions: Roughness significantly increased hydrophobicity (WCA), but had no effect on the number and metabolic activity of adherent and biofilm cells of C. albicans.

show abstract

“…Candida species, particularly C. albicans , which are a part of the human oral microbiota, have been reported as the main etiological agents responsible for the development of this inflammatory infection (6). Adherence of C. albicans to the host mucosal tissues (7) and also on the acrylic denture surfaces prepares reservoirs that produce proteolytic enzymes and damage mucosal tissues resulting in denture stomatitis (8). …”

Section: Introductionmentioning

confidence: 99%

Antifungal Effect of Zataria multiflora Essence on Experimentally Contaminated Acryl Resin Plates With Candida albicans

Jafari¹,

Tafti²,

Hoseiny³

et al. 2015

Iran Red Crescent Med J

View full text Add to dashboard Cite

Background:Adherence and colonization of Candida species particularly C. albicans on denture surfaces, forms a microbial biofilm, which may result denture stomatitis in complete denture users.Objectives:The purpose of the present study was to evaluate the antifungal effect Zataria multiflora essence in removing of Candida albicans biofilms on experimentally contaminated resin acryl plates.Materials and Methods:In the present experimental study, 160 resin acrylic plates (10 × 10 × 1 mm) were contaminated by immersion in 1 × 103 C. albicans suspension for 24 hours to prepare experimental Candida biofilms. The total number of Candida cells, which adhered to 20 randomly selected acryl resin plates was determined as the Candia load before cleaning. The remaining 140 plates were divided to seven groups of 20 and immersed in five concentrations of Zataria multiflora essence from 50 to 3.125 mg/mL as test, 100000 IU nystatin as the positive and sterile physiologic serum as the negative control. The remaining Candida cells on each acryl plate were also enumerated and data were analyzed using the SPSS 16 software with Kruskal-Wallis and Wilcoxon tests.Results:Zataria essence at concentrations of 50 and 25 mg/mL removed 100% of attached Candida cells similar to nystatine (MFC), while weaker Zataria essence solutions cleaned 88%, 60.5% and 44.7% of attached Candida cells. Kruskal-wallis test showed a statistically significant difference between all test groups (P = 0.0001). In this study 12.5 mg/mL concentration of Zataria multiflora was considered as the minimum inhibitory concentration (MIC90).Conclusions:Zataria essence, at concentrations of 50 and 25 mg/mL, effectively removed Candida cells that had adhered to the denture surface, similar to the level of removal observed for 100000 IU nystatin.

show abstract

Effects of surface roughness and dimorphism on the adhesion of Candida albicans to the surface of resins: scanning electron microscope analyses of mode and number of adhesions

Abstract: Adhesion of yeast-form C. albicans was indifferent to surface roughness. In contrast, mycelial adhesion increased with surface roughness of the resin because mycelia infiltrated the minute protuberances on rough surfaces.

Cited by 42 publications

References 16 publications

Development of antibacterial denture cleaner for brushing containing tea tree and lemongrass essential oils

Development of antibacterial denture cleaner for brushing containing tea tree and lemongrass essential oils

Effect of surface roughness on the hydrophobicity of a denture‐base acrylic resin andCandida albicanscolonization

Antifungal Effect of Zataria multiflora Essence on Experimentally Contaminated Acryl Resin Plates With Candida albicans

Contact Info

Product

Resources

About