Antifungal Effect of Zataria multiflora Essence on Experimentally Contaminated Acryl Resin Plates With Candida albicans

Abstract

Background:Adherence and colonization of Candida species particularly C. albicans on denture surfaces, forms a microbial biofilm, which may result denture stomatitis in complete denture users.Objectives:The purpose of the present study was to evaluate the antifungal effect Zataria multiflora essence in removing of Candida albicans biofilms on experimentally contaminated resin acryl plates.Materials and Methods:In the present experimental study, 160 resin acrylic plates (10 × 10 × 1 mm) were contaminated by immersion in 1 × 103 C. albicans suspension for 24 hours to prepare experimental Candida biofilms. The total number of Candida cells, which adhered to 20 randomly selected acryl resin plates was determined as the Candia load before cleaning. The remaining 140 plates were divided to seven groups of 20 and immersed in five concentrations of Zataria multiflora essence from 50 to 3.125 mg/mL as test, 100000 IU nystatin as the positive and sterile physiologic serum as the negative control. The remaining Candida cells on each acryl plate were also enumerated and data were analyzed using the SPSS 16 software with Kruskal-Wallis and Wilcoxon tests.Results:Zataria essence at concentrations of 50 and 25 mg/mL removed 100% of attached Candida cells similar to nystatine (MFC), while weaker Zataria essence solutions cleaned 88%, 60.5% and 44.7% of attached Candida cells. Kruskal-wallis test showed a statistically significant difference between all test groups (P = 0.0001). In this study 12.5 mg/mL concentration of Zataria multiflora was considered as the minimum inhibitory concentration (MIC90).Conclusions:Zataria essence, at concentrations of 50 and 25 mg/mL, effectively removed Candida cells that had adhered to the denture surface, similar to the level of removal observed for 100000 IU nystatin.

Antimicrobial Effects of Zataria multiflora Extract Mouthrinse on Orthodontic Elastomeric Ligatures- an In Vitro Study

“…Based on this study, a solution of 1% or 0.1% of ZM could be as effective as penicillin/nystatine (completely eliminating the S. mutans and C. albicans). Jafari et al (31) also reported that higher concentrations of ZM were more comparable to nystatine in regard to elimination of C. albicans of acrylic plates. The reason of testing very small percentages of ZM in this study was to obtain the minimum effective dosage of ZM since no study has been done so far on adverse effects and citotoxicity of ZM.…”

Estratégias de desinfecção de Candida albicans e os seus efeitos em resinas acrílicas: Uma revisão integrativa

“…Dentre os protocolos de desinfecção, a higienização mecânica por meio da escovação, assim como higienização química por imersão em soluções como HS, digluconato de clorexidina, hexetidina, perborato de sódio, vinagre, dentre outros, são as mais comuns (Castro et al, 2015;Sczepanski et al, 2014;Aoun et al, 2015). Paralelamente, métodos alternativos como a desinfecção pela energia de micro-ondas, por luz ultravioleta, por plasma e a utilização de produtos químicos de limpeza como sabonetes, pastilhas efervescentes e enxaguatórios bucais também vêm sendo utilizados para este fim (Asnaashari, Motamedi, Asnaashari, & Azari-Marhabi, 2019;Jafari, Tafti, Hoseiny, & Kazemi, 2015;Zoccolotti et al, 2018;Sharma, Garg, & Kalra, 2017;Asnaashari, Motamedi, Asnaashari, & Azari-Marhabi, 2019).…”

“…Métodos alternativos como substâncias fitoterápicas com atividade antimicrobiana têm sido alvo de estudos também, em função do seu potencial para prevenção de doenças bucais, especialmente os que se relacionam ao biofilme oral (Jafari et al, 2015;Madeira et al, 2016;Sookto et al, 2013;Oliveira et al, 2014).…”

Antifungal Effect of Thymus Vulgaris Essential Oil and Chlorhexidine on Candida Albicans Colonies Accumulated on Removable Orthodontic Appliances: A Double- blinded Clinical Trial.

“…Evidence shows that Zataria multi ora (Shirazi thyme) has suitable antimicrobial and antifungal properties for irrigation of root canals in endodontic treatment in vitro, compared with chemical irrigating solutions [38,39]. Also, Zataria multi ora essential oil is effective for disinfection of acrylic plates and orthodontic elastics in vitro [40,41]. Favorable antifungal activity of T. vulgaris essential oil, compared with CHX, has also been reported for disinfection of removable orthodontic appliances in vitro [42].…”