Effects of surface properties of titanium alloys modified by grinding, sandblasting and acidizing and nanosecond laser on cell proliferation and cytoskeleton

Wang, Yifei; Zhou, Yu; Li, Kangmei; Hu, Jun

doi:10.1016/j.apsusc.2019.144279

Cited by 37 publications

(15 citation statements)

References 45 publications

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“…Femtosecond laser, as a cold cauterization technology, did not observe the production of new crystal phases in our inspections. Some other studies have shown that sandblasting and femtosecond laser treatment bring alumina impurities, nitrogen or the thickened oxide layer of amorphous TiO2 and the accumulated carbon compounds to the titanium alloy substrate [ 44–47 ]. However, the latter three types of pollution are unavoidable reactions in the treatment process.…”

Section: Discussionmentioning

confidence: 99%

Characterization and evaluation of a femtosecond laser-induced osseointegration and an anti-inflammatory structure generated on a titanium alloy

Liu

Rui

Cheng

et al. 2021

Regenerative Biomaterials

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Cell–material interactions during early osseointegration of the bone–implant interface are critical and involve crosstalk between osteoblasts and osteoclasts. The surface properties of titanium implants also play a critical role in cell–material interactions. In this study, femtosecond laser treatment and sandblasting were used to alter the surface morphology, roughness and wettability of a titanium alloy. Osteoblasts and osteoclasts were then cultured on the resulting titanium alloy disks. Four disk groups were tested: a polished titanium alloy (pTi) control; a hydrophilic micro-dislocation titanium alloy (sandblasted Ti (STi)); a hydrophobic nano-mastoid Ti alloy (femtosecond laser-treated Ti (FTi)); and a hydrophilic hierarchical hybrid micro-/nanostructured Ti alloy [femtosecond laser-treated and sandblasted Ti (FSTi)]. The titanium surface treated by the femtosecond laser and sandblasting showed higher biomineralization activity and lower cytotoxicity in simulated body fluid and lactate dehydrogenase assays. Compared to the control surface, the multifunctional titanium surface induced a better cellular response in terms of proliferation, differentiation, mineralization and collagen secretion. Further investigation of macrophage polarization revealed that increased anti-inflammatory factor secretion and decreased proinflammatory factor secretion occurred in the early response of macrophages. Based on the above results, the synergistic effect of the surface properties produced an excellent cellular response at the bone–implant interface, which was mainly reflected by the promotion of early ossteointegration and macrophage polarization.

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Section: Discussionmentioning

confidence: 99%

Characterization and evaluation of a femtosecond laser-induced osseointegration and an anti-inflammatory structure generated on a titanium alloy

Liu

Rui

Cheng

et al. 2021

Regenerative Biomaterials

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“…The wettability is strongly connected with the surface roughness as indicated in the literature [41][42][43]. Surface wettability represents a key parameter: a high hydrophilicity, in fact, increases the surface energy and therefore the osseointegration.…”

Section: Wettabilitymentioning

confidence: 93%

Dual Electrochemical Treatments to Improve Properties of Ti6Al4V Alloy

et al. 2020

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Surface treatments are considered as a good alternative to increase biocompatibility and the lifetime of Ti-based alloys used for implants in the human body. The present research reports the comparison of bare and modified Ti6Al4V substrates on hydrophilicity and corrosion resistance properties in body fluid environment at 37 °C. Several surface treatments were conducted separately to obtain either a porous oxide layer using nanostructuration (N) in ethylene glycol containing fluoride solution, or bulk oxide thin films through heat treatment at 450 °C for 3 h (HT), or electrochemical oxidation at 1 V for 3 h (EO), as well as combined treatments (N-HT and N-EO). In-situ X-ray diffraction and ex-situ transmission electron microscopy have shown that heat treatment gave first rise to the formation of a 30 nm thick amorphous layer which crystallized in rutile around 620 °C. Electrochemical oxidations gave rise to a 10 nm thick amorphous film on the top of the surface (EO) or below the amorphous nanotube layer (N-EO). Dual treated samples presented similar results with a more stable behavior for N-EO. Finally, for both corrosion and hydrophilicity points of view, the new combined treatment to get a total amorphous N-EO sample seems to be the best and even better than the partially crystallized N-HT sample.

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“…Moreover, changes in nanotopography did not affect macrophage polarisation (Nano=Micro=Smooth), but did affect Streptococcus mutans attachment (Nano<Smooth<Micro). Wang et al 50 pre-treated Ti6Al4V flakes with sandpaper of different meshes, followed by treatment in three groups: grinding, sandblasting and etching, or nanosecond laser (Figure 2). When tested using rat bone marrow-derived mesenchymal stem cells (BMSCs), it was found that cell proliferation was not only affected by surface roughness, since cells tended to exhibit higher proliferation on flatter surfaces with a larger surface area.…”

Section: Mechanical Modification Methodsmentioning

confidence: 99%