Effects of Surface Morphology of ZnAl₂O₄ Ceramic Materials on Osteoblastic Cells Responses

Abstract

Ceramic scaffolds are widely studied in the tissue engineering field due to their potential in medical applications as bone substitutes or as bone-filling materials. The purpose of this study was to investigate the effect of surface morphology of nanostructure thin films of ZnAl 2 O 4 prepared by spray pyrolysis and bulk pellets of polycrystalline ZnAl 2 O 4 prepared by chemical coprecipitation reaction on the in vitro cell adhesion, viability, and cell-material interactions of osteoblastic cells. Our result showed that cell attachment was significantly enhanced from 60 to 80% on the ZnAl 2 O 4 nanostructured material surface when compared with bulk ceramic surfaces. Moreover, our results showed that the balance of morphological properties of the thin film nanostructure ceramic improves cell-material interaction with enhanced spreading and filopodia with multiple cellular extensions on the surface of the ceramic and enhancing cell viability/proliferation in comparison with bulk ceramic surfaces used as control. Altogether, these results suggest that zinc aluminate nanostructured materials have a great potential to be used in dental implant and bone substitute applications.

Zinc-Based Biomaterials for Regeneration and Therapy

“…Zn-based ceramic materials, including zinc oxide (ZnO), zinc sulphide (ZnS), zinc selenide (ZnSe), zinc phosphate (Zn 3 (PO 4 ) 2 ), and zinc aluminate (ZnAl 2 O 4 ), have been extensively explored in a variety of biomedical applications [7,56]. The major methods, characteristics, and biomedical applications of Zn based ceramic biomaterials are summarized (Table 1) and discussed in the following sections [57][58][59][60][61][62][63][64][65][66][67][68][69][70][71][72][73][74][75].…”

“…This provides a new promising direction for biodegradable coatings with Zn-based ceramic biomaterials. For example, Zn 3 (PO 4 ) 2 and ZnAl 2 O 4 coatings have demonstrated excellent cytocompatibility [73,74]. In addition, because of the released Zn ions, Zncontaining composites could potentially provide antibacterial properties for orthopedic implants to decrease or eliminate bacterial infections and the subsequent complications in the orthopedic surgery.…”

Synthesis of ZnAl2O4:(Er3+,Yb3+) spinel-type nanocrystalline upconverting luminescent marker in HeLa carcinoma cells, using a combustion aerosol method route

“…6). Suárez-Franco et al 42 examined the effect of the surface morphology of thin lms of ZnAl 2 O 4 prepared by spray pyrolysis and bulk pellets of polycrystalline ZnAl 2 O 4 prepared by a chemical co-precipitation reaction on the cell adhesion, viability, and cell-material interactions of osteoblastic cells. 18 Venkatachalam et al 40 reported the bioimaging of M1 cells (macrophages) using 30-40 nm Er 3+ doped Y 2 O 3 nanoparticles.…”

“…Hemmer et al 41 reported the synthesis of bare and poly(ethylene glycol)-b-poly(acrylic acid) (PEG-b-PAAc) modied Gd 2 O 3 :Er 3+ ,Yb 3+ nanostructures and a study of the cytotoxicity by incubation with B-cell hybridomas and macrophages, in vivo. Suárez-Franco et al 42 examined the effect of the surface morphology of thin lms of ZnAl 2 O 4 prepared by spray pyrolysis and bulk pellets of polycrystalline ZnAl 2 O 4 prepared by a chemical co-precipitation reaction on the cell adhesion, viability, and cell-material interactions of osteoblastic cells.…”

Tunable Chemical and Topographic Patterns Based on Binary Colloidal Crystals (BCCs) to Modulate MG63 Cell Growth

“…Furthermore, there is the potential to coat implantable medical devices that require a mimic of the extracellular matrix (ECM) and stem cell niche . To date, there have been a variety of surface topographies that have been used such as hexagonal close packed (hcp) colloidal crystal patterns, microgrooved surfaces, and nanostructured thin films of ZnAl 2 O 4 to name a few . In addition, particles of different chemical composition like glass, silica (Si), titanium (Ti), gold, aluminum alloy, carbon‐based composite materials, polycarbonate membranes, and many more have already been explored in osteoblast‐like cell culture.…”