Effects of sulfhydryl groups and oxygen tension on the cell proliferating activity of bovine serum albumin in culture.

Kan, Mikio; Yamane, Isao

doi:10.1247/csf.7.133

Cited by 6 publications

(3 citation statements)

References 16 publications

(19 reference statements)

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“…Serumfree medium RITC 80-7 with FN and BSA also supported growth, but after 7 days, growth declined and cell lysis took place. As previously reported, HEL cells cultured in this serum-free medium showed lysis in an atmospheric oxygen environment but not in a low (7%) oxygen environment (Kan and Yamane 1982b). Therefore, the growth kinetics in this serum-free medium was also surveyed in a low (7%) oxygen environment.…”

Section: Growth Kineticsmentioning

confidence: 58%

Primary culture of human esophageal epithelial cells.

Katayama

Akaishi

Nishihira

et al. 1984

Tohoku J. Exp. Med.

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Section: Growth Kineticsmentioning

confidence: 58%

Primary culture of human esophageal epithelial cells.

Katayama

Akaishi

Nishihira

et al. 1984

Tohoku J. Exp. Med.

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“…We previously reported that BSA promotes the growth of human diploid fibroblasts in serum-free culture in a hypoxic environment (11,27). The activity of BSA, however, declined in an aerobic environment during a 5-day culture period (12). When HSA was added to the culture medium, the oxygen-dependent reduction in growth was more marked than in BSA-supplemented medium, but this reduction could be partially regulated by an addition of dithiothreitol (DTT) in the medium (12).…”

Section: Resultsmentioning

confidence: 95%

“…The activity of BSA, however, declined in an aerobic environment during a 5-day culture period (12). When HSA was added to the culture medium, the oxygen-dependent reduction in growth was more marked than in BSA-supplemented medium, but this reduction could be partially regulated by an addition of dithiothreitol (DTT) in the medium (12). Therefore all the cultures used were made with an addition of DTT (2 mg/1).…”

Section: Resultsmentioning

confidence: 99%

Effects of ceruloplasmin and the haptoglobin-hemoglobin complex on the oxygen-dependent reduction in growth of human diploid fibroblasts in serum-free, albumin containing medium.

Kan

Yabe

Yamane

et al. 1983

Cell Struct. Funct.

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ABSTRACT. We examined that growth-promoting activity of two different human albumin (HSA) preparations for human diploid fibroblasts in serumfree RITC 80-7 medium. The activity of one preparation (sample A) was affected markedly by environmental oxygen, whereas the other (sample B) was little affected. Sample B contained ceruloplasmin (Cp) and haptoglobin (Hp) as impurities. To detect the generation of superoxide anion in the media the amount of reduction of cytochrome c that is inhibited by superoxide dismutase (SOD) was determined. In an aerobic environment it was relatively large in comparison with reduction inhibited in a hypoxic environment. Reduction in the sample A with HSA-supplemented medium was relatively large in comparison with that in sample B with HSA-supplemented medium. The reduction of cytochrome c also was inhibited by Cp (25 mg/1) and catalase (4000 units/ml). Moreover, SOD, Cp, catalase and Hp.Hb (but not Hp) partially prevented oxygen-dependent reduction in growth in an aerobic environment when added to sample A HSA-supplemented medium. These results suggest that Cp and Hp.Hb act as an antioxidants in culture.We developed a serum-free culture medium RITC 80-7, for human diploid fibroblasts and succeeded in the long term culture of these cells using this medium supplemented with human fibronectin (10 mg/l, FN) and bovine serum albumin (5 g/l, BSA, Cohn's fraction V) in a hypoxic (7 %) environment (11,27). Similar results were obtained with human serum albumin (HSA) instead of BSA. The growth promoting activities of both BSA and HSA were affected markedly by the oxygen tension in the culture environment. In an aerobic environment, a reduction in cell growth and a decrease in the in vitro lifespan were apparent in comparison to results in a hypoxic environment. The degree of reduction differed for each BSA commercial preparation (28). In contrast, cells cultured with 10 % fetal bovine serum (FBS)-supplemented

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Chemically defined medium for the production of biologically active substances of CHO cells

et al. 1992

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A recombinant CHO cell line (GT19) secreting a high level of human growth hormone (hGH) was constructed with amplification of the introduced hGH gene. The cells grew well in the alpha MEM medium supplemented with 5% dialyzed fetal calf serum (dFCS), but not with less than 1% dFCS. Therefore we examined various medium components and obtained an improved medium which supported cell growth at low serum concentrations. The production of hGH by the cells was also enhanced in this medium.

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Effects of sulfhydryl groups and oxygen tension on the cell proliferating activity of bovine serum albumin in culture.

Cited by 6 publications

References 16 publications

Primary culture of human esophageal epithelial cells.

Primary culture of human esophageal epithelial cells.

Effects of ceruloplasmin and the haptoglobin-hemoglobin complex on the oxygen-dependent reduction in growth of human diploid fibroblasts in serum-free, albumin containing medium.

Chemically defined medium for the production of biologically active substances of CHO cells

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