Effects of stocking density on oxidative stress status and mammary gland permeability in early lactating dairy cows

Lin, Shixin; Liu, Jianxin; Wang, Kaiying; Wang, Diming

doi:10.1111/asj.13206

Cited by 6 publications

(3 citation statements)

References 45 publications

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“…Briefly, milk plasmin activity was determined with pyro-GLU-Phe-Lysp-nitroanilide hydroxychloride chromogenic substrate [2.5 mg/mL; Biophen CS-41(03), Aniara, Westchester, OH] in a 96-well plate (Sarstedt, Helsingborg, Sweden). To evaluate oxidative stress status in the mammary gland, milk malondialdehyde (MDA, Lin et al, 2019 was measured with a Spectra Max M5 microplate reader and commercial kit.…”

Section: Methodsmentioning

confidence: 99%

Nano-sized zinc addition enhanced mammary zinc translocation without altering health status of dairy cows

et al. 2021

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This study aimed to evaluate role of nano-sized zinc (Zn) on lactation performance, health status, and mammary permeability of lactating dairy cows. Thirty multiparous dairy cows with similar days in milk (158 ± 43.2) and body weight (694 ± 60.5 kg) were chosen based on parity and milk production and were randomly assigned to 3 treatment groups: basal diet (control, 69.6 mg/kg of Zn adequate in Zn requirement), basal diet additional Zn-methionine (Zn-Met, providing 40 mg/kg of Zn), and basal diet additional nano-sized Zn oxide (nZnO, providing 40 mg/kg of Zn). The study lasted for 10 wk, with the first 2 wk as adaptation. Feed intake, milk yield and the related variables, and plasma variables were determined every other week. Blood hematological profiles were determined in the 8th week of the study. We found that feed intake, milk yield, and milk composition were similar across the 3 groups. The nZnO- and Zn-Met-fed cows had greater milk Zn concentrations in the milk (3.89 mg/L (Zn-Met) and 3.93 mg/L (nZnO)) and plasma (1.25 mg/L (Zn-Met) and 1.29 mg/L (nZnO)) than the control cows (3.79 mg/L in milk and 1.21 mg/L in plasma). The nZnO-fed cows had higher Zn concentrations in plasma but not in milk compared to Zn-Met-fed cows. The Zn appearance in milk was greater in nZnO-fed (area under curve during the first 4 h post-feeding for milk Zn: 16.1 mg/L) and Zn-Met-fed cows (15.7 mg/L) than in control cows (15.0 mg/L). During the first 4 h post-feeding, milk to blood Zn ratio was greater in nZnO-fed animals but lower in Zn-Met-fed cows compared with control cows. Oxidative stress-related variables in plasma, blood hematological profiles, and mammary permeability related variables were not different across treatments. In summary, lactation performance, Zn concentrations in milk and plasma, hematological profiles, mammary permeability were similar in cows fed nZnO and Zn-Met. We therefore suggested that nZnO feeding can improve Zn bioavailability without impairing lactation performance, health status, and mammary gland permeability in dairy cows.

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Section: Methodsmentioning

confidence: 99%

Nano-sized zinc addition enhanced mammary zinc translocation without altering health status of dairy cows

et al. 2021

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“…Due to the synthesis and secretion of large amounts of milk during lactation, aerobic metabolic activities are significantly increased, and there is a risk of excessive free radical generation. Excessive free radicals can cause oxidative damage, decrease the antioxidant function and immune function of dairy cow mammary epithelial cells, affect the normal lactation function of mammary cells, and finally lead to a decrease in milk production and milk quality (3,(14)(15)(16). Therefore, improving the antioxidant capacity of bovine mammary cells may be an effective therapeutic strategy against OS.…”

Section: Discussionmentioning

confidence: 99%

Green Tea Polyphenols Alleviate Hydrogen Peroxide-Induced Oxidative Stress, Inflammation, and Apoptosis in Bovine Mammary Epithelial Cells by Activating ERK1/2–NFE2L2–HMOX1 Pathways

et al. 2022

Front. Vet. Sci.

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Oxidative stress (OS) is one of the main limiting factors affecting the length of lactation and milk quality in dairy cows. For high-producing dairy cows, the OS of mammary glands is a serious problem. Green tea polyphenols (GTP), found mainly in tea, are a combination of many phenols. GTP have a good effect on antioxidation, inflammation resistance, obesity, fat cell metabolism improvement, and lowering of blood lipid. Therefore, we studied the role of GTP on OS in dairy cows and further investigated whether GTP alleviates oxidative damage of bovine mammary epithelial cells (BMECs) induced by hydrogen peroxide (H2O2) and its underlying molecular mechanism. In this study, 500 μM of H2O2 for 12 h incubation was chosen as the condition of the OS model of BMECs. In addition, the present results found that treatment with GTP alleviated the oxidative damage induced by H2O2 [the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) were significantly increased, and the contents of malondialdehyde (MDA), 8-isoprostaglandin (8-iso-PG), 8-oxo-deoxyguanosine (8-OHdG), and protein carbonyl (PC) and caspase-3 and caspase-9 activities were significantly reduced]. These effects are related to the activation of the erythrocyte-derived nuclear factor 2-like protein 2 (NFE2L2) signaling pathway and the inactivation of the caspase/Bcl-2 apoptotic pathway. When NFE2L2 short interfering RNA (siRNA) was used to downregulate the expression of NFE2L2 in cultured BMECs, NFE2L2-siRNA transfection abolished the protective effect of GTP on H2O2-induced intracellular reactive oxygen species (ROS) accumulation and apoptosis. In addition, the mitogen-activated protein kinase (MAPK) inhibition test further proved that GTP relieved H2O2-induced oxidative damage by activating the NFE2L2 signaling pathway, which was achieved by activating the extracellular-regulated kinase 1/2 (ERK1/2) signaling pathway. Overall, the results indicate that GTP has a beneficial effect on the redox balance of BMECs. In addition, GTP might be a latent antioxidant in vivo, which can be administered to ruminants during stressful periods such as the perinatal period.

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“…Moreover, the pH and HCO 3 − in the blood were considered to be strongly associated with clinical assessment scores that separate healthy calves from diarrheic ones [ 13 ]. Across dairy cows with different physiological stages, early lactating cows, especially high-yielding ones, are suspected to be in a sub-healthy status [ 14 ], experiencing symptoms such as mammary gland leakage [ 15 , 16 ] and mammary peroxidation [ 17 ], which ultimately lead to mammary dysfunction or even mastitis. However, index evaluation refers to mammary health status, including mammary oxidative stress, mammary permeability, and milk SCC, which largely rely on laboratory facilities.…”

Section: Introductionmentioning

confidence: 99%

Multiple-Vessel-Based Blood Gas Profiles Analysis Revealed the Potential of Blood Oxygen in Mammary Vein as Indicator of Mammary Gland Health Risk of High-Yielding Dairy Cows

Feng

Peng

et al. 2022

Animals

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The blood gas profile is a routine method in the rapid disease diagnosis of farm animals, yet its potential in evaluating mammary health status of dairy cows remains to be investigated. This study was conducted to learn the potential of the blood gas parameter regarding the mammary gland health status in lactating dairy cows. Twenty animals were divided into two groups, the H-SCC group (milk SCC > 122 k/mL) and L-SCC group (milk SCC < 73.8 k/mL), to compare blood gas profiles from different blood vessels and to identify the key parameters associated with milk somatic cell count. H-SCC cows are higher in malondialdehyde content, but lower in SOD and T-AOC activities in the milk, compared to the L-SCC group. In terms of blood gas parameters, most differ across the three vessels, including K+, CO2 pressure, O2 pressure, HCO3−, base excess in the extracellular fluid compartment, and saturation of O2. The Pearson correlation analysis showed that oxygen-related variables in the mammary vein, including oxygen concentrations, O2 pressure, and saturation of O2, are negatively correlated with levels of malondialdehyde, lactate dehydrogenase, and plasmin in the milk. Our study revealed that oxygen-related variables in the mammary vein can be a marker in suggesting mammary-gland health status in high-yielding cows.

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Effects of stocking density on oxidative stress status and mammary gland permeability in early lactating dairy cows

Cited by 6 publications

References 45 publications

Nano-sized zinc addition enhanced mammary zinc translocation without altering health status of dairy cows

Nano-sized zinc addition enhanced mammary zinc translocation without altering health status of dairy cows

Green Tea Polyphenols Alleviate Hydrogen Peroxide-Induced Oxidative Stress, Inflammation, and Apoptosis in Bovine Mammary Epithelial Cells by Activating ERK1/2–NFE2L2–HMOX1 Pathways

Multiple-Vessel-Based Blood Gas Profiles Analysis Revealed the Potential of Blood Oxygen in Mammary Vein as Indicator of Mammary Gland Health Risk of High-Yielding Dairy Cows

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