Effects of Stigmasterol and β-Sitosterol on Nonalcoholic Fatty Liver Disease in a Mouse Model: A Lipidomic Analysis

Feng, Simin; Gan, Ling; Yang, Chung S.; Liu, Anna B.; Lu, Wenyun; Shao, Ping; Dai, Zhuqing; Sun, Peilong; Luo, Zisheng

doi:10.1021/acs.jafc.7b06146

Cited by 83 publications

(60 citation statements)

References 45 publications

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“…PCA is an unsupervised multivariable statistical method to discriminate the inter-relationships among different groups in a multivariate pattern (Feng, Gan, et al, 2018). In Figure 3a The results were consistent with those from chemical composition analysis ( Figure 1) and antioxidation analysis ( Table 2).…”

Section: Gc-mssupporting

confidence: 78%

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Chemical composition and antioxidant capacities analysis of different parts of Brasenia schreberi

Feng

Ning

Luan

et al. 2019

J Food Process Preserv

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The objective of this study was to investigate how parts and maturity of Brasenia schreberi could affect its chemical composition and bioactivities. Among different parts of B. schreberi, G‐BS sample contained the highest total carbohydrate content (589.1 ± 11.07 mg/g) and total polyphenols content (mg GA/g), while it had relatively low HO· (IC50 = 3.42 ± 0.12 mg/ml), DPPH (IC50 = 4.39 ± 0.24 mg/ml) and ABTS free radical (IC50 = 1.66 ± 0.05 mg/ml) scavenging activity. Principal components analysis showed that mucilage covered B. schreberi was significantly different from the plant itself. Galactose (61.56 ± 3.29 mg/g), fucose (36.45 ± 0.68 mg/g), and arabinose (27.08 ± 0.27 mg/g) were three top abundant monosaccharides in G‐BS sample. G‐BS contained about 57% of polysaccharides and it showed potential hypoglycemic activities as it exhibited comparable α‐amylase (IC50 = 3.35 mg/ml) and α‐glucosidase (IC50 = 1.94 mg/ml) inhibitory activity to acarbose. G‐BS might be a good source of polysaccharide that can be used as functional supplement. Practical applications As parts and maturity can affect chemical composition and bioactivity, present results help guide potential application of B. schreberi. The study will help food industry to develop B. schreberi polysaccharide‐based products as a functional additive or pharmaceutical supplement.

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Section: Gc-mssupporting

confidence: 78%

“…PCA is an unsupervised multivariable statistical method to discriminate the inter‐relationships among different groups in a multivariate pattern (Feng, Gan, et al, ). In Figure a, the first principal component factor efficiently separated G‐BS samples from ML‐BSP, TL‐BSP, and TS‐BSP.…”

Section: Discussionmentioning

confidence: 99%

Chemical composition and antioxidant capacities analysis of different parts of Brasenia schreberi

Feng

Ning

Luan

et al. 2019

J Food Process Preserv

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“…Obviously, the concentrations of β‐sitosterol in A. pernyi pupae oil (3.36 ± 0.19 mg g −1 ) and B. mori pupae oil (3.34 ± 0.16 mg g −1 ) were higher than those in peanut oil (1.61–1.72 mg g −1 ) and soybean oil (1.55–1.75 mg g −1 ) . It is documented that β‐sitosterol contributes to lowering of the levels of serum total cholesterol and low‐density lipoprotein cholesterol . Therefore, silkworm pupae oil acts as a potential functional oil due to the cholesterol‐lowering effects.…”

Section: Resultsmentioning

confidence: 99%

Comprehensive Identification of Principal Lipid Classes and Tocochromanols in Silkworm (Antheraea pernyi and Bombyx mori) Pupae Oils

Wang

Zou

et al. 2019

Euro J Lipid Sci & Tech

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A comprehensive identification of lipid compositions and tocochromanols inAntheraea pernyi (A. pernyi) and Bombyx mori (B. mori) pupae oil is reported in the present study. Fatty acid profiling shows that both oils contain high levels (79.67% vs 71.11%) of unsaturated fatty acids, especially linoleic acid and linolenic acid. Moreover, linolenic acid is preferentially enriched at the sn-2 positions of triacylglycerols (TAGs). Liquid chromatograph-mass spectrometry (LC-MS) analysis demonstrates that POO (TAG with one palmitoyl and two oleoyls) is the primary TAG form with percentages of 20.18% in A. pernyi and 15.00% in B. mori. The dominating phospholipid species are phosphatidylcholine (PC, 30.40% vs 54.61%) and phosphatidylethanolamine (PE, 34.82% vs 20.39%). Four sterol constituents with total contents of 382.56 ± 3.12 and 371.65 ± 2.98 µg g −1 are identified and analyzed quantitatively. Additionally, the levels of tocochromanols (20.15 ± 0.89 vs 17.15 ± 0.71 mg g −1 ) are quantified in both silkworm pupae oils. Overall, silkworm oil acts as an enriched source of functional lipids and tocochromanols. Practical Applications: A systematic investigation on the principal lipid classes and tocochromanols of Antheraea pernyi pupae and Bombyx mori pupae oil is reported in this study.

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“…The presented method can easily be utilized for non-AT samples containing high amounts of neutral lipids, e.g. tissue samples from subjects with fatty-liver disease [40], [41]. Such samples, under common extraction and measurement conditions, would interfere with full lipidomic analysis due to ion suppression.…”

Section: Discussionmentioning

confidence: 99%

Comprehensive and quantitative analysis of white and brown adipose tissue by shotgun lipidomics

Grzybek

Palladini

Alexaki

et al. 2019

Molecular Metabolism

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Objective Shotgun lipidomics enables an extensive analysis of lipids from tissues and fluids. Each specimen requires appropriate extraction and processing procedures to ensure good coverage and reproducible quantification of the lipidome. Adipose tissue (AT) has become a research focus with regard to its involvement in obesity-related pathologies. However, the quantification of the AT lipidome is particularly challenging due to the predominance of triacylglycerides, which elicit high ion suppression of the remaining lipid classes. Methods We present a new and validated method for shotgun lipidomics of AT, which tailors the lipid extraction procedure to the target specimen and features high reproducibility with a linear dynamic range of at least 4 orders of magnitude for all lipid classes. Results Utilizing this method, we observed tissue-specific and diet-related differences in three AT types (brown, gonadal, inguinal subcutaneous) from lean and obese mice. Brown AT exhibited a distinct lipidomic profile with the greatest lipid class diversity and responded to high-fat diet by altering its lipid composition, which shifted towards that of white AT. Moreover, diet-induced obesity promoted an overall remodeling of the lipidome, where all three AT types featured a significant increase in longer and more unsaturated triacylglyceride and phospholipid species. Conclusions The here presented method facilitates reproducible systematic lipidomic profiling of AT and could be integrated with further –omics approaches used in (pre-) clinical research, in order to advance the understanding of the molecular metabolic dynamics involved in the pathogenesis of obesity-associated disorders.

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Effects of Stigmasterol and β-Sitosterol on Nonalcoholic Fatty Liver Disease in a Mouse Model: A Lipidomic Analysis

Cited by 83 publications

References 45 publications

Chemical composition and antioxidant capacities analysis of different parts of Brasenia schreberi

Chemical composition and antioxidant capacities analysis of different parts of Brasenia schreberi

Comprehensive Identification of Principal Lipid Classes and Tocochromanols in Silkworm (Antheraea pernyi and Bombyx mori) Pupae Oils

Comprehensive and quantitative analysis of white and brown adipose tissue by shotgun lipidomics

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