Effects of steroid hormones on bovine polymorphonuclear leukocyte function

Roth, James A.

doi:10.31274/rtd-180813-12474

Cited by 25 publications

(35 citation statements)

References 96 publications

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“…Administration of 1-24ACTH caused the lack of these differences, suggesting that during stress, neutrophils are less reactive to chemokines. These results are in compliance with those detected in sheep during chronic stress but not with other studies, most likely depending on different experimental protocols [10,21].…”

Section: Discussionsupporting

confidence: 92%

“…PMN were isolated according to the procedure suggested by Carlson and Kaneko [3], widely used for ruminant PMN isolation [3,19,21], using plastic tubes to avoid PMN adhesion to glass surfaces: blood from the EDTA coated tubes was divided into 4 aliquots of 10 mL, and after centrifugation (15 min, 1 000 × g), plasma and the upper layer (3 mm) of RBC were removed: a hypotonic lysis of RBC was carried out by adding 20 mL of distilled water to each tube. After gently mixing (30 s), a hypertonic NaCl solution (2.7 g/dL in phosphate buffer 0.0132 M, pH 7.2) was added to restore isotonicity.…”

Section: Pmn Isolationmentioning

confidence: 99%

“…Data regarding phagocyte functions in spontaneous or experimentally induced stress, are often contradictory and depend on the type of stressor used and on the step of the phagocytic process studied [1,7,8,12,[17][18][19]21]. However the different steps of the phagocytic process have not yet been simultaneously evaluated.…”

Section: Introductionmentioning

confidence: 99%

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Effect of 1-24ACTH administration on sheep blood granulocyte functions

Paltrinieri¹,

Panelli²,

Comazzi³

et al. 2002

Vet. Res.

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-The objective of the present study was to determine the efficiency of blood neutrophils (PMN) taken from sheep during acute stress. Ten healthy Charolle sheep were sampled before treatment (T0) and 1 (T1), 2 (T2), 24 (T24) and 48 (T48) hours after 1-24ACTH administration. Ten sheep serving as the controls were sampled at the same time intervals, using saline solution instead of 1-24ACTH. At each time sampling, rectal temperature, heart rate, cortisol, glucose, non-esterified fatty acids (NEFA), total and differential leukocyte counts were evaluated. PMN were isolated after centrifugation of whole blood and hypotonic lysis of RBC. Chemotaxis was evaluated on a modified Boyden chamber using a nitrate cellulose filter and both Zymosan activated serum (ZAS) and interleukin-8 (IL-8) as chemoattractants. Phagocytosis was measured using both non-opsonized latex beads and fluoresceinated yeasts opsonized with homologous serum. Superoxide( ) O 2 -production was evaluated by measuring superoxide dismutase-inhibitable reduction of ferricytochrome C, and adherence by a colorimetric assay of acid phosphatase activity of adherent cells. The administration of 1-24ACTH induced an acute stress reaction, indicated by the presence of clinical, biochemical and hematological changes. Adherence significantly increased from T0 to T2 in treated sheep. This might be responsible for the depression of non-specific immunity in stressed animals. Studies using stressors other than 1-24 ACTH are needed to verify the influence of other components of the stress reaction on PMN functions.sheep / PMN / ACTH / stress / phagocytosis Résumé -Effets de l'administration de 1-24ACTH sur la fonction des granulocytes sanguins de brebis. L'objectif de cette étude a été la détermination de l'efficacité des neutrophiles (PMN) préle-vés à des brebis pendant un stress aigu. Pour cela, nous avons effectué des prélèvements de sang à 10 brebis Charolle saines avant d'administrer du 1-24ACTH (T0) et, de nouveau, 1 (T1), 2 (T2), 24 (T24), 48 (T48) heures après ce traitement. Comme contrôle nous avons fait des prélèvements de sang à 10 brebis auxquelles nous avions administré de la solution saline au lieu de 1-24ACTH. A l'occasion de chaque prélèvement nous avons déterminé la température, la fréquence cardiaque et les niveaux sanguins de cortisol, glucose, acides gras non-esterifiés, des leucocytes totaux et différenciés. Nous avons isolé les PMN après centrifugation du sang et lyse hypotonique des hématies. Afin de vé-rifier l'activité chimiotactique des PMN, nous avons utilisé une chambre de Boyden modifiée ainsi que du sérum activé par le Zymosan (ZAS) et de l'interleukine-8 (IL-8) comme chimioattractants. Afin de mesurer la phagocytose, nous avons utilisé aussi bien des particules de latex non-opsonisées que de la levure opsonisée avec du sérum homologue marqué à la fluorescéine. Ensuite, nous avons quantifié la production d'anion superoxyde en mesurant la réduction (inhibée par l'enzyme superoxyde dismutase) du ferricytochrome C, et l'adhérence (par ...

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Section: Discussionsupporting

confidence: 92%

Section: Pmn Isolationmentioning

confidence: 99%

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Effect of 1-24ACTH administration on sheep blood granulocyte functions

Paltrinieri¹,

Panelli²,

Comazzi³

et al. 2002

Vet. Res.

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“…The immunosuppressive effect of dexamethasone administration was revealed by the decreased blastogenic response of PBL, and the reduced activity of PMN in accordance with previous findings in man (Chretien & Garagusi, 1972;Fauci, 1980) and cattle (Roth & Kaeberle, 1981;Roth et al, 1982). The suppressive effect on leukocyte reactivity has been shown to occur by a number of different mechanisms.…”

Section: Discussionsupporting

confidence: 89%

Chronic Shedding of Bovine Enteric Coronavirus Antigen-Antibody Complexes by Clinically Normal Cows

Crouch¹,

Ohmann

Watts³

et al. 1985

Journal of General Virology

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SUMMARYUsing an ELISA for the detection of virus-specific immune complexes, ten cows were found to be shedding bovine enteric coronavirus. The shedding patterns from five of these animals were followed for a period of 12 weeks, and all were found to be chronically shedding virus. Despite the presence of both faecal and serum antibody the infection was not cleared; therefore, the role of cell-mediated immunity (CMI) was investigated by immunosuppressing the chronically shedding cows with dexamethasone. No major role for CMI in maintaining the chronic infection could be determined, although immunosuppression did result in a temporary reduction in the shedding of virus-specific immune complexes.

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“…Os índices do cortisol no grupo dos ovinos, que receberam a monensina retornaram aos valores pré-estabelecidos 48h pós-indução; porém, este achado não foi observado no grupo controle, nos quais os animais apresentaram a concentração desta variável significativamente (p<0,05) superiores. É reconhecido que a elevação dos níveis de cortisol causa in vivo a inibição do metabolismo oxidativo dos neutrófilos (Roth et al 1982). Portanto, é bastante provável que o aumento de neutrófilos NBT-positivos no grupo monensina após 48h da indução da acidose esteja relacionada à diminuição do estresse, fato este não observado no grupo controle.…”

Section: Resul Resul Resul Resul Results T T T Tados E Discus Ados E Dunclassified