Chronic Shedding of Bovine Enteric Coronavirus Antigen-Antibody Complexes by Clinically Normal Cows

Crouch, Colin; Ohmann, H. Bielefeldt; Watts, Trent; Babiuk, Lorne A.

doi:10.1099/0022-1317-66-7-1489

Cited by 58 publications

(57 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Other studies reported the presence of Coronavirus in scouring calves, for example 14% in southern Britain (Reynolds et al, 1986), 4% in Scotland (Snodgrass et al, 1986) and 9% in The Netherlands (Moerman et al, 1982). The presence of Coronavirus in the faecal matter of clinically healthy calves is in agreement with previous reports (Crouch et al, 1985).…”

Section: Discussionsupporting

confidence: 93%

Infectious agents associated with diarrhoea of calves in the canton of Tilarán, Costa Rica

Pérez

Kummeling

Janssen

et al. 1998

Preventive Veterinary Medicine

View full text Add to dashboard Cite

A case-control study of calves under 3 months of age was carried out by weekly visits to 15 farms in the canton of Tilarán, Costa Rica. Most farms were dedicated to beef or dual-purpose (DP) production. Faecal samples were collected over a 6-month period from a total of 194 calves with clinical signs and from 186 animals without clinical signs of diarrhoea as assessed by a scoring system. The samples were investigated for the presence of viruses, bacteria and parasites. Torovirus was detected for the first time in Costa Rica and was present in 14% of calves with diarrhoea and in 6% of the controls. Coronavirus and Rotavirus were less frequently encountered in either one of the groups (in 9 and 7% of scouring calves and in 1 and 2% of controls, respectively). Escherichia coli was detected in 94% of all the faecal samples, but isolates from only three samples from calves with diarrhoea contained the K99 antigen. Similarly, Salmonella was found only in scouring calves. Cryptosporidium oocysts were detected in animals with signs of diarrhoea, while other coccidia oocysts, Strongylida and Strongyloides eggs were frequently found in animals both with and without diarrhoea. A conditional logistic regression (CLR) analysis to compare healthy and scouring calves showed a significant difference with regard to the presence of Torovirus, Rotavirus and Coronavirus.

show abstract

Section: Discussionsupporting

confidence: 93%

Infectious agents associated with diarrhoea of calves in the canton of Tilarán, Costa Rica

Pérez

Kummeling

Janssen

et al. 1998

Preventive Veterinary Medicine

View full text Add to dashboard Cite

show abstract

“…Althought BCoV isolates are generally recognized as being associated with enteric diseases of newborn calves (NCD: neonatal calf diarrhea) (Mebus et al, 1973) and winter dysentery (WD) or chronic diarrhea in adult cattle (Crouch et al, 1985;Benfield and Saif, 1990), there exist much evidences that other isolates may be responsible or contribute to acute respiratory problems in feedlot cattle (Reynolds et al, 1985;Storz et al, 1992). A recent survey conducted in feedlots or livestock shows in several states in the USA, emphasized the role of RBCoV in the bovine shipping fever syndrome pneumonia (Storz et al, 1996(Storz et al, , 2000.…”

mentioning

confidence: 99%

Bovine coronaviruses associated with enteric and respiratory diseases in Canadian dairy cattle display different reactivities to anti-HE monoclonal antibodies and distinct amino acid changes in their HE, S and ns4.9 protein

et al. 2001

View full text Add to dashboard Cite

Bovine coronavirus isolates associated with recent outbreaks of respiratory disease in Ontario and Quebec dairy farms were compared to reference strains known to be responsible for neonatal calf diarrhea (NCD) or winter dysentery (WD) of adult cattle. In respect to their hemagglutinating properties and their higher RDE activities with rat erythrocytes, WDBCoV strains differed from NCDBCoV strains and respiratory bovine coronaviruses RBCoV strains. Serologically, three MAbs directed to the HE glycoprotein of the WDBCoV strain BCQ.2590 recognized two serogroups amongst NCDBCoV strains by hemagglutination inhibition, whereas only one of the MAbs failed to react toward three of the four RBCoV isolates tested. Sequencing analysis of the S (S1 portion), HE, ORF4 and ORF5 genes of BCoV isolates associated with different clinical syndromes indicated that neither insertions or deletions could explain their distinct tropism. For the HE glycoprotein, a total of 15 amino acids (aa) substitutions were identified by comparing field isolates to the prototype Mebus strain. Two specific proline substitutions were identified for virulent strains being located in the signal peptides (aa 5) and aa position 367; one specific aa change was revealed at position 66 for RBCoV field isolates. Analysis of the S1 portion of the S glycoprotein revealed a total of eight aa changes specific to enteropathogenic (EBCoV) strains and eight aa changes specific to RBCoV strains. For all BCoV isolates studied, the region located between the S and M genes (ORF4) apparently encodes for two non-structural (ns) proteins of 4.9 and 4.8 kDa. A specific non-sense mutation was identified for the nucleotide at position 88 of the putative 4.9 kDa protein gene of RBCoV isolates resulting in 29 rather that 43 aa residues. The ORF5, which encodes a 12.7 ns protein and the 9.5 kDa E protein, was highly conserved amongst the BCoV field isolates.

show abstract

“…Bovine coronavirus (BCV) is an enteric virus which multiplies in the differentiated enterocytes of the small intestine and colon, causing acute enteritis in newborn calves (Stair et aL, 1972;Babiuk et al, 1985) and chronic shedding (Crouch et al, 1985) or diarrhoea (Takahashi et aL, 1980) in adult cows. BCV particles consist of large single-stranded positive RNA (30 kb) associated with a basic phosphonucleoprotein (N, Mr 52K) forming a helical ribonucleoprotein protected by a viral envelope containing three glycoproteins, M, S and HE (Laporte & Bobulesco, 1981;King & Brian, 1982;.…”

Section: Introductionmentioning

confidence: 99%

Bovine coronavirus peplomer glycoproteins: detailed antigenic analyses of S1, S2 and HE

Vautherot

Madelaine

Boireau

et al. 1992

Journal of General Virology

View full text Add to dashboard Cite

Chronic Shedding of Bovine Enteric Coronavirus Antigen-Antibody Complexes by Clinically Normal Cows

Cited by 58 publications

References 19 publications

Infectious agents associated with diarrhoea of calves in the canton of Tilarán, Costa Rica

Infectious agents associated with diarrhoea of calves in the canton of Tilarán, Costa Rica

Bovine coronaviruses associated with enteric and respiratory diseases in Canadian dairy cattle display different reactivities to anti-HE monoclonal antibodies and distinct amino acid changes in their HE, S and ns4.9 protein

Bovine coronavirus peplomer glycoproteins: detailed antigenic analyses of S1, S2 and HE

Contact Info

Product

Resources

About