Effects of Stabilized Rice Bran on Obesity and Antioxidative Enzyme Activity in High Fat Diet-induced Obese C57BL/6 Mice

Abstract

Rice bran (RB), a by-product obtained during polishing of un-milled rice, contains a large quantity of essential nutrients such as minerals, vitamins, fiber, amino acids, and antioxidants. In this study, the anti-obesity effects of stabilized RB addition as a food material in a diet-induced obese animal model were investigated. For the analysis, a total of 32 mice were randomly divided into four groups: normal diet group (ND, n=8), high-fat diet group (HFD, n=8), 20% RB added high fat diet group (HFRB20, n=8), and 40% RB added high fat diet group (HFRB40, n=8). The animals were sacrificed after 7 weeks. Body weight gain, various adipose tissue weights, blood glucose concentration, and serum leptin level of the HFRB40 group were found to be significantly lower than those of the HFD group. Moreover, antioxidant enzyme activity of the HFRB40 group was significantly higher than that of the HFD group. However, the HFRB20 group did not show significantly different values. The results of this study show that RB (40%) addition reduces body weight gain and improves obesity-related parameters. These results suggest RB could be considered as a functional additive material for the manufacture of anti-obesity products, and 40% RB addition would be the physiologically effective level.

Rice Bran Fermented with Kimchi-Derived Lactic Acid Bacteria Prevents Metabolic Complications in Mice on a High-Fat and -Cholesterol Diet

“…Alauddin et al [ 40 ] also reported that the suppression of weight gain was greater in mice given fermented RB than in those given raw RB. Our results correspond to past findings that providing study animals a high-fat diet with RB or stabilized RB leads to lower weight gain compared to the control group [ 18 , 55 , 56 ] and that only a high-cholesterol diet with RB suppresses weight gain, with other grain brans being unable to suppress weight gain [ 57 ]. Adding bran unsaponifiable matter or rice bran polysaccharide to a high-fat diet also affects weight change [ 58 , 59 ].…”

“…RB water extract was reported to prevent metabolic syndrome by increasing insulin resistance and inhibiting abdominal fat accumulation when orally administered to mice for four weeks with a high-fat diet [ 56 ]. Stabilized RB has been reported to reduce blood glucose in rats and humans [ 11 , 18 ]. Further, feeding diabetic mice RB-extracted γ-oryzanol reduced fasting glucose and insulin concentration [ 86 ], and consuming β-sistosterol in RB was also found to reduce blood glucose [ 87 ].…”

“…RB fibers improve intestine functions [ 9 ] and reduce blood glucose levels [ 10 , 11 ], and RB oil has anti-oxidant effects, suppresses the elevation of blood pressure [ 12 ], and relieves chronic inflammation [ 13 ]. In addition, γ-oryzanol and ferulic acid in RB improve lipid metabolism [ 14 , 15 ] and have antioxidant effects [ 5 , 16 , 17 , 18 ]. Thus, there is growing interest in RB as a potential functional food ingredient amid new research findings on various bioactive substances in RB and their effects.…”

Anti-obesity effects of boiled tuna extract in mice with obesity induced by a high-fat diet

“…CAT, an enzyme that plays a role in the reduction of hydrogen peroxide with water, forms an antioxidant enzyme system with SOD. In general, increases in various metabolic processes induce oxidative stress and activate SOD and CAT (23); in obesity, the levels of these enzymes are decreased (24). In this study, the SOD and CAT levels in the livers of the mice in all groups are shown in Fig.…”

Effects of Allium hookeri root water extracts on inhibition of adipogenesis and GLUT-4 expression in 3T3-L1 adipocytes

“…Adipose tissues composed of adipocytes and small amounts of other types of cells may be involved in lipid metabolism for lipid secretion and storage, and in glucose metabolism for insulindependent glucose uptake, and also may control the endocrine system for hormone and cytokine secretion (5,6). However, dysfunction of adipose tissues, such as excessive differentiation of adipocytes, may increase the risk of obesity and diabetes (7).…”