Effects of SSd Combined with Radiation on Inhibiting SMMC-7721 Hepatoma Cell Growth

Wang, Baofeng; Lin, Shuai; Bai, Ming; Song, Lingqin; Wang, Min; Wang, Meng; Yang, Pengfei; Ma, Hongbing; Wang, Xijing

doi:10.12659/msm.891355

Cited by 10 publications

(15 citation statements)

References 13 publications

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“…First, SSd increases the expression of p53 and Bax (Liu & Li 2014 ; Wang et al. 2014a, 2014b ; Yao et al. 2014 ), activates caspases apoptosis pathway, including the activation of caspases-3 and caspases-7 (Chiang et al.…”

Section: Ssdmentioning

confidence: 99%

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A systematic review of the active saikosaponins and extracts isolated from Radix Bupleuri and their applications

Yuan

Yang

et al. 2016

Pharmaceutical Biology

154

111

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Context: Radix Bupleuri has been used in traditional Chinese medicine for over 2000 years with functions of relieving exterior syndrome, clearing heat, regulating liver-qi, and lifting yang-qi. More natural active compounds, especially saikosaponins, have been isolated from Radix Bupleuri, which possess various valuable pharmacological activities.Objective: To summarize the current knowledge on pharmacological activities, mechanisms and applications of extracts and saikosaponins isolated from Radix Bupleuri, and obtain new insights for further research and development of Radix Bupleuri.Methods: PubMed, Web of Science, Science Direct, Research Gate, Academic Journals and Google Scholar were used as information sources through the inclusion of the search terms ‘Radix Bupleuri’, ‘Bupleurum’, ‘saikosaponins’, ‘Radix Bupleuri preparation’, and their combinations, mainly from the year 2008 to 2016 without language restriction. Clinical preparations containing Radix Bupleuri were collected from official website of China Food and Drug Administration (CFDA).Results and conclusion: 296 papers were searched and 128 papers were reviewed. A broad spectrum of in vitro and in vivo research has proved that Radix Bupleuri extracts, saikosaponin a, saikosaponin d, saikosaponin c, and saikosaponin b2, exhibit evident anti-inflammatory, antitumor, antiviral, anti-allergic, immunoregulation, and neuroregulation activities mainly through NF-κB, MAPK or other pathways. 15 clinical preparations approved by CFDA remarkably broaden the application of Radix Bupleuri. The main side effect of Radix Bupleuri is liver damage when the dosage is excess, which indicates that the maximum tolerated dose is critical for clinical use of Radix Bupleuri extract and purified compounds.

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Section: Ssdmentioning

confidence: 99%

“…Second, SSd decreases the expression of B cell lymphoma 2 (Bcl-2) family proteins (Liu & Li 2014 ; Wang et al. 2014a, 2014b ; Yao et al. 2014 ), suppresses the expression of COX-2, which has been shown to be involved in carcinogenesis (Lu et al.…”

Section: Ssdmentioning

confidence: 99%

A systematic review of the active saikosaponins and extracts isolated from Radix Bupleuri and their applications

Yuan

Yang

et al. 2016

Pharmaceutical Biology

154

111

View full text Add to dashboard Cite

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“…Saikosaponin D (SSd) is one of the major Saponin components derived from the dried roots of Bupleurum falactum, a traditional Chinese medicine plant. It has been reported that SSd exerts anticancer activities in cervical and lung cancer cells, and hepatocellular carcinoma cells (9)(10)(11)(12). SSd may potentiate tumor necrosis factor (TNF)-α-mediated cell death via suppression of TNF-α-induced nuclear factor (NF)-κB activation, while inducing apoptosis by enhancing the loss of mitochondrial membrane potential (13).…”

Section: Introductionmentioning

confidence: 99%

“…Although previous studies have indicated that SSd exerts anticancer activities in various tumor cell lines (9)(10)(11)(12), the effects of it in central nervous system (CNS) malignant tumors remain unknown. In the present study, the growth potential, proliferation inhibition and apoptosis induction effects of SSd on human U87 glioma cells were investigated.…”

Section: Introductionmentioning

confidence: 99%

Effects of Saikosaponin D on apoptosis in human U87 glioblastoma cells

Cai

Zhang

et al. 2017

Molecular Medicine Reports

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Saikosaponin D (SSd) is a type of Saponin derivative, which is a component extracted from Bupleurum falactum. SSd has been reported to exert anticancer activities. However, the effects of SSd on gliomas have not been elucidated. The aim of the present study was to investigate the pharmacological functions and potential molecular mechanisms of SSd in human U87 glioblastoma cells. The cells were treated with SSd at various concentrations for 48 h, the cell viability was assessed with 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium assay, and the activation of Akt, extracellular signal‑regulated kinases (ERK), c‑Jun N‑terminal kinases (JNK) and caspase‑3 was assessed by western blotting. In addition, apoptosis levels were analyzed with Hoechst 33258 and Annexin V staining. The results demonstrated that treatment of the U87 glioma cells with SSd markedly suppressed cell proliferation in a dose‑dependent manner. Meanwhile, SSd treatment enhanced apoptosis in the U87 cells. Furthermore, SSd significantly inhibited the phosphorylation of Akt and ERK, and promoted phosphorylated‑JNK and cleaved caspase‑3 expression. The present study revealed the potential therapeutic effects of SSd in the treatment of gliomas, and the cytotoxic effects of SSd in U87 cells were at least partly attributed to the depression of phosphatidylinositol 3‑kinase/Akt and ERK protein expression levels, and activation of JNK and caspase-3 expression.

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“…For patients who are not suitable for resection or chemotherapy, radiotherapy is a common effective method; this involves delivering radioisotopes through either a percutaneous or transarterial approach in order to reach the nidus and obtain high tumoricidal activity [62,63]. However, hepatoma cells generally have a lower sensitivity to radioisotopes than other cell types that leads to unsatisfactory curative effects [64]. Radiotherapy also often causes some adverse reactions, such as fever, nausea, chills, poor appetite, and tiredness [65,66].…”

Section: Combination Of Tcm and Radiotherapy For Treatment Of Hccmentioning

confidence: 99%

Traditional Chinese medicine combined with other therapies for treatment of hepatocellular carcinoma in clinical trials

Feng¹,

Zhu²,

Hu³

et al. 2017

Cancer Rep Rev

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Hepatocellular carcinoma (HCC) is one of the most common malignant tumors worldwide with a high mortality, and still there are only few effective methods to treat it. To this end, alternative medicines from traditional Chinese medicine (TCM) are being investigated for their ability to eliminate the tumor or halt its progression. A large number of studies have shown that TCM can be an effective approach to treat the HCC in clinical trials when used alone or in combination with other therapies. Thus, TCM has made significant progress, and has begun to gain worldwide popularity for promoting healthcare and HCC treatment. Because of this progress, periodic summaries are needed to facilitate further research for the use of TCM to treat HCC. This paper provides a comprehensive summary of this work with regard to the following aspects: herbalist views on the etiology and therapeutic principles for treatment of HCC, treating HCC by TCM alone, treating HCC with TCM in combination with resection, chemotherapy, radiotherapy and interventional therapy, and other therapies. Additionally, the current main problems and future application prospects for treatment of HCC by TCM described, which could provide scientific guidance for clinician as well as references for the treatment of other cancers.

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Effects of SSd Combined with Radiation on Inhibiting SMMC-7721 Hepatoma Cell Growth

Cited by 10 publications

References 13 publications

A systematic review of the active saikosaponins and extracts isolated from Radix Bupleuri and their applications

A systematic review of the active saikosaponins and extracts isolated from Radix Bupleuri and their applications

Effects of Saikosaponin D on apoptosis in human U87 glioblastoma cells

Traditional Chinese medicine combined with other therapies for treatment of hepatocellular carcinoma in clinical trials

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