Effects of specimen handling and otolith preparation on concentration of elements in fish otoliths

Proctor, Craig; Thresher, Ronald E.

doi:10.1007/s002270050360

Cited by 93 publications

(82 citation statements)

References 19 publications

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“…The heads were then removed and preserved in 95% ethanol for later removal of the otoliths. Proctor & Thresher (1998) found little difference between preservation methods such as freezing and ethanol immersion on otolith Ca concentration, and variability in Sr:Ca ratio was due largely to variability in Sr concentration.…”

Section: Methodsmentioning

confidence: 77%

Migratory behaviour and habitat use by American eels Anguilla rostrata as revealed by otolith microchemistry

Jessop¹,

Shiao²,

Iizuka³

et al. 2002

Mar. Ecol. Prog. Ser.

130

161

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Section: Methodsmentioning

confidence: 77%

Migratory behaviour and habitat use by American eels Anguilla rostrata as revealed by otolith microchemistry

Jessop¹,

Shiao²,

Iizuka³

et al. 2002

Mar. Ecol. Prog. Ser.

130

161

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“…This method of storage has been shown to have the least impact on elemental concentrations (Milton & Chenery 1998). Proctor & Thresher (1998) showed that a delay in the removal of the otolith of just 3 h may influence the concentrations of a range of elements including sodium, potassium, calcium and strontium. In this study, otoliths were collected freshly from the dead specimens upon sorting of the catch and the abovementioned elements were not included in the analysis.…”

Section: Methodsmentioning

confidence: 99%

Otolith geochemistry indicates life-long spatial population structuring in a deep-sea fish, Coryphaenoides rupestris

Longmore¹,

Trueman²,

Neat³

et al. 2011

Mar. Ecol. Prog. Ser.

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Little is understood about connectivity of deep-sea fish populations. Analysis of the geochemical properties of fish otoliths is one way to draw inferences regarding their movements and habitat use in the marine environment. Trace element and stable isotope analyses of otoliths were undertaken to assess patterns of spatial and temporal population structure of a wide-ranging deepwater fish, the roundnose grenadier Coryphaenoides rupestris. Fish were sampled from 4 locations across the distribution range of the species in the northeast Atlantic. Multivariate analyses of elemental ratios (Li/Ca, Mn/Ca, Ba/Ca, Zn/Ca, Cu/Ca) revealed strong geographic separation at each life stage, and an overall significant difference between life stages. Otolith oxygen (δ 18 O) and carbon (δ 13 C) stable isotope analysis indicated a depth migration (and reduction in metabolic activity) from relatively shallow in the juvenile phase to much deeper in the adult phase at all locations. The results suggest that roundnose grenadier are comprised of geographically distinct population units that persist throughout their life-history, migrating deeper as they get older.

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“…1). To minimise the effects of differences among sample collections of handling artefacts on measurements of trace metal concentrations, both saggittae were taken as soon as possible after collection (Proctor & Thresher 1998, Milton & Chenery 1998. Fish were kept on ice when it was impractical to remove otoliths immediately after capture.…”

Section: Methodsmentioning

confidence: 99%

“…The otolith was then polished to the core with 1200 grade wet and dry sandpaper moistened with doubledistilled water. Water use was kept to a minimum during otolith preparation to minimise and standardise any preparation bias (Proctor & Thresher 1998). To remove any contamination, the surface was wiped vigorously after polishing with a piece of tissue paper moistened with 0.5 M aristar nitric acid.…”

Section: Methodsmentioning

confidence: 99%

Can otolith chemistry detect the population structure of the shad hilsa Tenualosa ilisha? Comparison with the results of genetic and morphological studies

Milton¹,

Chenery²

2001

Mar. Ecol. Prog. Ser.

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The stock structure of the valuable tropical shad Tenualosa ilisha ('hilsa') has been studied in Bangladesh and India by analysing morphometric and genetic data. However, these studies had a narrow geographic scope and their results conflict. We made a comprehensive study of the stock structure of hilsa with otolith microchemistry in conjunction with complementary genetic and morphometric studies of the same fish. We examined the trace-element composition of the otolith cores of hilsa with laser-ablation inductively coupled plasma mass spectrometry. The otoliths of fish from 19 collections at 13 sites in Bangladesh and 6 collections at 4 sites from elsewhere within the species' range (Kuwait, SE India, Myanmar and Sumatra) were analysed for 8 trace elements. Samples were collected from Bangladesh mainly during 2 comprehensive surveys (1996 and 1997). When these data were analysed separately, there were significant differences in otolith composition among sites. However, when both years' data were analysed together, there were few significant differences among sites, and some sites separated by hundreds of kilometres that were sampled in different seasons and years had very similar compositions. This was in spite of both large seasonal intra-site and between-site differences in water chemistry. Repeat samples from 5 sites (4 in Bangladesh) showed that differences in otolith composition at a single site were significant and of similar magnitude to that found among sites. Our results support the conclusion from allozyme studies that there is extensive movement and mixing of hilsa throughout Bangladesh, and therefore the population should be managed as a single stock. Genetic and otolith data both showed that hilsa from SE India and Myanmar were not significantly different from fish collected in coastal areas of Bangladesh, and suggest that hilsa in the Bay of Bengal were a single stock. Both methods also separated fish from Sumatra and Kuwait from other sites, providing strong evidence of separate stocks in those regions. In contrast, morphometric studies separate fish from several nearby sites in Bangladesh, but these differences are likely to be largely due to phenotypic variability and are unlikely to be geneticallybased. Our results suggest that otolith microchemistry may be a good proxy for genetic structure at large scales where differences in water chemistry are highly likely. However, for sedentary species and those without distinct spawning and non-breeding areas, it requires both comprehensive and repeated sampling at finer scales before any confidence should be placed in the results.

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Effects of specimen handling and otolith preparation on concentration of elements in fish otoliths

Cited by 93 publications

References 19 publications

Migratory behaviour and habitat use by American eels Anguilla rostrata as revealed by otolith microchemistry

Migratory behaviour and habitat use by American eels Anguilla rostrata as revealed by otolith microchemistry

Otolith geochemistry indicates life-long spatial population structuring in a deep-sea fish, Coryphaenoides rupestris

Can otolith chemistry detect the population structure of the shad hilsa Tenualosa ilisha? Comparison with the results of genetic and morphological studies

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