Can otolith chemistry detect the population structure of the shad hilsa Tenualosa ilisha? Comparison with the results of genetic and morphological studies

Abstract

The stock structure of the valuable tropical shad Tenualosa ilisha ('hilsa') has been studied in Bangladesh and India by analysing morphometric and genetic data. However, these studies had a narrow geographic scope and their results conflict. We made a comprehensive study of the stock structure of hilsa with otolith microchemistry in conjunction with complementary genetic and morphometric studies of the same fish. We examined the trace-element composition of the otolith cores of hilsa with laser-ablation inductively coupled plasma mass spectrometry. The otoliths of fish from 19 collections at 13 sites in Bangladesh and 6 collections at 4 sites from elsewhere within the species' range (Kuwait, SE India, Myanmar and Sumatra) were analysed for 8 trace elements. Samples were collected from Bangladesh mainly during 2 comprehensive surveys (1996 and 1997). When these data were analysed separately, there were significant differences in otolith composition among sites. However, when both years' data were analysed together, there were few significant differences among sites, and some sites separated by hundreds of kilometres that were sampled in different seasons and years had very similar compositions. This was in spite of both large seasonal intra-site and between-site differences in water chemistry. Repeat samples from 5 sites (4 in Bangladesh) showed that differences in otolith composition at a single site were significant and of similar magnitude to that found among sites. Our results support the conclusion from allozyme studies that there is extensive movement and mixing of hilsa throughout Bangladesh, and therefore the population should be managed as a single stock. Genetic and otolith data both showed that hilsa from SE India and Myanmar were not significantly different from fish collected in coastal areas of Bangladesh, and suggest that hilsa in the Bay of Bengal were a single stock. Both methods also separated fish from Sumatra and Kuwait from other sites, providing strong evidence of separate stocks in those regions. In contrast, morphometric studies separate fish from several nearby sites in Bangladesh, but these differences are likely to be largely due to phenotypic variability and are unlikely to be geneticallybased. Our results suggest that otolith microchemistry may be a good proxy for genetic structure at large scales where differences in water chemistry are highly likely. However, for sedentary species and those without distinct spawning and non-breeding areas, it requires both comprehensive and repeated sampling at finer scales before any confidence should be placed in the results.

Otolith geochemistry indicates life-long spatial population structuring in a deep-sea fish, Coryphaenoides rupestris

“…Ba is known to be correlated with biological activity on the overlying surface waters (Swan et al 2006) and also to increase in concentration with depth and distance from shore (Swan et al 2003a). High Li levels are thought to relate to input from hydrothermal activity (Bruland 1983) and to co-vary with salinity (Milton et al 1997, Campana et al 2000, Milton & Chenery 2001, Gillanders 2002, Rooker et al 2003. In the present study, Mn was found in highest concentrations in the Skagerrak and lowest on the MAR.…”

Food traceability using the 87Sr/86Sr isotopic ratio mass spectrometry

“…Hilsa from four regions in Bangladesh showed widespread movement among regions, and all fish examined had spent several months in water with marine Sr isotope ratios. The data supported previous findings [134,135] that hilsa from the remote population in Sylhet were distinct and showed that fish from the region had a common homing strategy. A study carried out by an American team [136] aimed to quantify the relative contribution of water and food to Sr and Ba deposited in otoliths of juvenile mummichogs (F. heteroclitus).…”

Elevated levels of trace elements in cores of otoliths and their potential for use as natural tags

“…To quantify these records and address a number of questions in marine ecology, researchers have utilized inductively coupled plasma mass spectrometry (ICPMS) (Campana 1999, Campana & Thorrold 2001. This approach has been used to differentiate among spawning stocks (Edmonds et al 1991, Milton & Chenery 2001a, Rooker et al 2001, identify individuals that utilized different nursery habitats , Forrester & Swearer 2002, Gillanders 2002, and discriminate between dispersed and retained larvae (Swearer et al 1999). However, to determine the natal site of an individual, the site where a given larva was spawned, 2 pieces of information are required: (1) a spatial atlas of otolith chemical signatures at natal sites, ideally generated using otoliths of near-term eggs or recently hatched larvae from multiple discrete sites prior to dispersal, and (2) the ability to measure that natal chemical signature from post-larval recruits.…”