Effects of soybean isoflavone on metabolism of rat osteoblasts and cytokines in vitro

Abstract

The effects and mechanisms of soybean isoflavone on osteoblast (OB) proliferation in vitro were investigated. Fifty female Wistar rats were randomly divided into five groups with 10 rats in each group. Rat OBs were separated and cultured. The first generation of OBs cultured for 48 hr at various concentrations of isoflavone were set as the experimental groups, the OBs exposed to estradiol (E 2 ) culture were considered as positive control group. The biological characterization of OBs was investigated by phase contrast microscopy and alkaline phosphatase (ALP) histochemistry. The concentrations of interleukin (IL-1), osteoprotegerin (OPG), transforming growth factor (TGF), granulocytemacrophage colony-stimulating factor (GM-CSF), and vascular endothelial growth factor (VEGF) in isoflavone culture solutions were determined. Proliferation rate of OBs was increased in experimental group comparing that in the blank group. ALP activity in experimental group was higher than that in blank group. No significant differences of ALP activity were observed between E 2 culture group and isoflavone group at concentrations of 10 −5 and 10 −7 mM (P > 0.05). Furthermore, in the experimental groups at low isoflavone concentrations, the concentrations of OPG, TGF, and VEGF were increased and positively correlated with OB proliferation. However, the concentrations of IL-1, GM-CSF were decreased at higher concentration of isoflavone and were negatively correlated with OB proliferation. Soybean isoflavone could promote the growth and proliferation of rat OB, it might act as the stimulator of OPG, TGF, and VEGF pathway, and the inhibitor of IL-1, GM-CSF pathway as well.

Antioxidant Effect of Soymilk Fermented by Lactobacillus plantarum HFY01 on D-Galactose-Induced Premature Aging Mouse Model

“…Unfermented soybean products mainly exist in the form of glycosides, and fermented soybean products mainly exist in the form of aglycone, which has higher bioavailability, as reflected in this experiment (Figure 10). Soybean isoflavones are good antioxidants and tumor suppressors, especially for hormone-related tumors, such as breast cancer and prostate cancer; they can reduce blood cholesterol levels, coronary atherosclerosis, and peripheral arterial vascular damage, and their weak estrogen-like effect has a certain preventive effect on menopausal syndrome and bone loss in women (52,53). In addition, soybean isoflavones can improve lipid metabolism, and they can enhance nonspecific immunity and anti-inflammatory effects, mainly related to daidzein and genistein (54,55).…”

Natural Drugs as a Treatment Strategy for Cardiovascular Disease through the Regulation of Oxidative Stress

“…Soybean isoflavone (SI) is a bioactive secondary metabolite formed during soybean growth. Studies have shown that SI can protect the cardiovascular system through its antioxidant effects [ 195 – 197 ]. SI enhanced the activity of SOD, decreased the level of thiobarbituric acid reactant in plasma, and enhanced the antioxidant capacity of plasma, as well as the activity of GSH-PX in erythrocytes [ 198 ].…”

Bioactivity, Molecular Mechanism, and Targeted Delivery of Flavonoids for Bone Loss

“…It has a molecular structure comparable to estrogen and exists in nature as a molecule with a polyphenolic hydroxyl group called β-glucoside and can be induced by estrogen. Owing to the antiestrogenic and estrogenic activities, it is often referred to as a selective estrogen receptor modulator [ 50 , 51 ]. These compounds may be protective against osteoporosis due to their ability to exert osteogenic and antiresorptive actions on bone, particularly on bone turnover and growth [ 52 , 53 ].…”