Effects of Some Pesticides on Development of Ascaris suum Eggs

Abstract

To evaluate the effects of pesticides to parasite eggs, Ascaris suum eggs were incubated with 5 different pesticides (1:1,500-1:2,000 dilutions of 2% emamectin benzoate, 5% spinetoram, 5% indoxacarb, 1% deltamethrin, and 5% flufenoxuron; all v/v) at 20℃ for 6 weeks, and microscopically evaluated the egg survival and development on a weekly basis. The survival rate of A. suum eggs incubated in normal saline (control eggs) was 90±3% at 6 weeks. However, the survival rates of eggs treated with pesticides were 75-85% at this time, thus significantly lower than the control value. Larval development in control eggs commenced at 3 weeks, and 73±3% of eggs had internal larvae at 6 weeks. Larvae were evident in pesticide-treated eggs at 3-4 weeks, and the proportions of eggs carrying larvae at 6 weeks (36±3%-54±3%) were significantly lower than that of the control group. Thus, pesticides tested at levels similar to those used in agricultural practices exhibited low-level ovicidal activity and delayed embryogenesis of A. suum eggs, although some differences were evident among the tested pesticides.

Assessment of the inhibitory effects of disinfectants on the embryonation of Ascaridia columbae eggs

“…In two separate studies on Ascaris suum of pigs, 10% povidone iodine and some pesticides that are commonly used in agriculture were proved effective in inhibiting or delaying embryogenesis of A . suum eggs [20,21].…”

Study of disinvasive properties of innovative aldehyde disinfectant

“…Today, composite preparations consisting of several active substances from different groups of chemical compounds are preferred, which, due to the synergism of the components, have a broader range of activity and are effective at much lower concentrations, are more economically advantageous (Zavgorodniy et al, 2013;Koski, Anttila and Kuusela, 2015;.…”

“…One of the key conditions that affect the validity of the results obtained from the test of the disinfectant is the use of reference test cultures. In order to evaluate the disinvasive effect of drugs, it is advisable to use the eggs of Ascaris suum (Pecson and Nelson, 2005;Yu et al, 2014). This test culture is used to test not only chemicals but also to evaluate the ovicidal activity of physical factors (Brownell and Nelson, 2006).…”