Effects of Some Non-ionic Surfactants on Transepithelial Permeability in Caco-2 Cells

Dimitrijevic, D; Shaw, Andrew J.; Florence, Alexander T.

doi:10.1211/0022357001773805

Cited by 126 publications

(89 citation statements)

References 18 publications

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“…The P-80 diet induced NAFLD and intestinal inflammation and was correlated with a significant reduction in acetate, propionate and butyrate concentration in fecal samples. P-80 also altered bile acid levels, which influences microbiota composition [27]. The serum metabolites of P-80 fed mice and control mice were analyzed by LC-MS. Interestingly the level of DCA a secondary bile acid carried out by gut bacteria such as Clostridium Cluster XI and XIV was substantially increased.…”

Section: Discussionmentioning

confidence: 99%

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Food Additive P-80 Impacts Mouse Gut Microbiota Promoting Intestinal Inflammation, Obesity and Liver Dysfunction

Singh¹

2016

SOJMID

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Open Access Research Articlethe globalization of unhealthy lifestyle. Key metabolic and physiological changes, including obesity, metabolic syndrome and Nonalcoholic Fatty Liver Disease (NAFLD) are commonly associated with chronic, non-communicable disease that are responsible for approximately 70% of the world's death.There are compelling arguments to include the composition of gut microbiota as a key risk factor for the development of obesity and obesity related metabolic disorders, including insulin resistance, type 2 diabetes, dyslipidemia and non-alcoholic fatty liver disease [3]. The gut microbiota has been implicated in having both direct (i.e. through effect on nutrient uptake) and indirect effect on health [4,5]. In mice, food additives have been demonstrated to affect the host microbiota interaction, leading to low-grade inflammation, adiposity and adiposity associated metabolic effect [6].We set out to elucidate whether alteration in the gut microbial community after P-80 ingestion is a causative agent of liver dysfunction. To explore this possibility, we setup an experiment to examine the impact of P-80 on gut microbiota using wild-type C57BL/ 6 mice. The experimental mice were gavaged with the amount of P-80 equal to 1% of their body weight. This amount correlates to the acceptable daily human intake of 1% per kg of polysorbate-80 to body weight [7]. Methods Mice and dietThe C57BL/6 (000664) mice were purchased from The Jackson Laboratory. The mice were maintained under specific pathogenfree conditions, on a 12-h light-dark cycle, and fed normal diet (12 kcal% fat, 29 kcal% protein, 59% kcal carbohydrate) ad libitum. For this study there were two groups: one control group (ingesting saline via gavage) and one experimental P-80 fed group (ingesting P-80 dissolve in saline, 1% per kg via gavage). This dose corresponds to the FDA Acceptable Daily Intake (ADI) in humans (1% per kg (body weight), adjusted to the mouse AbstractThe increasing prevalence of obesity has emerged as one of the most important global public health issue. The change to the human microbiome as a result of changes in the quality and quantity of food intake over the past several decades has been implicated in the development of obesity and metabolic syndrome. We administered polysorbate-80 to mice via gavage. The researchers monitor liver noninvasively using a bioluminescence imaging. For the liver dysfunction we measure the liver enzymes and PAS stain on liver, electron microscopy liver mitochondria. For the assessment of intestinal inflammation we measured fecal LCN2, LPS, MPO and flagellin by ELISA and qPCR. We use confocal microscopy to detect closet bacteria near the epithelium. 16S sequence was used for the composition of microbiota. Compared with control mice, those receiving emulsifier, showed impaired glycemic tolerance, hyperinsulinemia, altered liver enzymes, larger mitochondria and increased gall bladder size. Additionally, mice in the experimental group showed higher levels of DCA, reduced Muc2 RNA expression, reduced m...

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Section: Discussionmentioning

confidence: 99%

“…Imbalance in the composition of the intestinal microbiota (known as dysbiosis) can be caused by immune deficits and dietary influences [27]. We investigated the possibility that intestinal bacteria play a key role in NAFLD development.…”

Section: Rrna and Serum Metabolitementioning

confidence: 99%

Food Additive P-80 Impacts Mouse Gut Microbiota Promoting Intestinal Inflammation, Obesity and Liver Dysfunction

Singh¹

2016

SOJMID

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“…In addition, Tween 20 has been reported to increase tight junction permeability, leading to enhanced drug absorption by the paracellular pathway. 31 The pharmacokinetic data in male rats imply that PLHloaded SMEDDS developed in this study could improve the dissolution and absorption of PLH.…”

Section: In Vivo Absorption Studiesmentioning

confidence: 81%

Self-microemulsifying drug-delivery system for improved oral bioavailability of pranlukast hemihydrate: preparation and evaluation

Baek¹,

Lee²,

Cho³

et al. 2013

IJN

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Abstract:The purpose of the present investigation was to develop and evaluate a selfmicroemulsifying drug delivery system (SMEDDS) for improving the oral absorption of a pranlukast hemihydrate (PLH), a very poorly water-soluble drug. An efficient self-microemulsifying vehicle for PLH was selected and optimized using solubility testing and phase diagram construction. The formulations were characterized by assessing self-emulsification performance, droplet size analysis, in vitro drug release characteristics and formulation stability studies. Optimized formulations for in vitro dissolution and bioavailability assessment were Triethylcitrate (TEC; 10%), Tween 20 (50%), Span 20 (25%), triethanolamine (5%), and benzyl alcohol (10%). The SMEDDS readily released the lipid phase to form a fine oil-in-water microemulsion with a narrow distribution size. Saturated solubilities of PLH from SMEDDS in water, pH 4.0 and 6.8, were over 150 times greater than that of plain PLH. The release of 100% PLH from SMEDDS was considerably greater compared to only 1.12% in simulated intestinal fluid (pH 6.8) from plain PLH after 2 hours. The PLH suspension with 0.5% sodium carboxymethylcellulose or 3% PLH-loaded SMEDDS was administrated at a dose of 40 mg/kg as PLH to fasted rats. The absorption of PLH from SMEDDS resulted in about a threefold increase in bioavailability compared with plain PLH aqueous suspension. Our studies illustrated that the potential use of the new SMEDDS can be used as a possible alternative to oral delivery of a poorly water-soluble drug such as PLH.

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“…In this sense it was also observed that the increase of the cytotoxic effect was dependent on surfactant concentration but not on the exposition time. This finding was due to the fact that the cytotoxic action of unpurified NSVs was determined by the presence of the free molecules of surfactants, which were able to exert immediately their cytotoxic action during the incubation period by noticeably perturbing the cellular membranes (Dimitrijevic et al, 2000;Lin et al, 2007) and hence causing the cellular death. This hypothesis was strongly supported by the evidence that HLF cells treated with the free surfactants showed a greater (P<0.001) cytotoxic insult than those treated with unpurified non-ionic surfactant vesicles.…”

Section: Case Studiesmentioning

confidence: 99%

Novel Concept in Pulmonary Delivery

Carafa¹,

Marianecci²,

Paolino³

et al. 2012

Chronic Obstructive Pulmonary Disease - Current Concepts and Practice

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Effects of Some Non-ionic Surfactants on Transepithelial Permeability in Caco-2 Cells

Cited by 126 publications

References 18 publications

Food Additive P-80 Impacts Mouse Gut Microbiota Promoting Intestinal Inflammation, Obesity and Liver Dysfunction

Food Additive P-80 Impacts Mouse Gut Microbiota Promoting Intestinal Inflammation, Obesity and Liver Dysfunction

Self-microemulsifying drug-delivery system for improved oral bioavailability of pranlukast hemihydrate: preparation and evaluation

Novel Concept in Pulmonary Delivery

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