Effects of Some Growth Substances on Mitosis

Prasad, G.; Das, Kaushik

doi:10.1508/cytologia.42.323

Cited by 13 publications

(11 citation statements)

References 9 publications

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“…The results are in clear agreement with observations of Mohandas and Grant (1972); Tomkins and Grant (1976), Prasad and Das (1977) and Kumari and Vaidyanath (1989).…”

Section: Resultssupporting

confidence: 92%

Does 2,4-dichlorophenoxyacetic acid (2,4-D) induce genotoxic effects in tissue cultured Allium roots?

et al. 2016

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2,4-Dichlorophenoxyacetic acid (2,4-D) is a synthetic plant growth regulator that is highly toxic to most broad leaved plants and relatively nontoxic to monocotyledonous plants; is frequently used as weed killer. The study aimed to investigate cytogenetic effects of different concentrations of 2,4-D (0.67, 1.34, 2.01, 2.68, 3.35 and 4.02 mg/L) on Allium cepa bulblets' root tips treated for 24 and 48 h. The results showed six types of structural aberrations: C-mitosis, stickiness, laggards, bridges, fragments and multipolarity that varied numerically compared to control. It significantly affected mitotic index (MI) at 24 and 48 h treatment. In the Allium test, MI increased significantly at three lower concentrations (0.67, 1.34, 2.01 mg/L) after treatment with 2,4-D for 24 h and decreased significantly at higher concentration. Whereas, 2,4-D treatment for 48 h increased MI at all concentrations with significantly decreased MI at the highest concentration. The experiment was extended using comet test that did not reveal significant difference among treatments except for application of 4.02 mg/L 2,4-D for 48 h; where cell damages were verified by comet test. Rest of the concentrations for any duration of time were not damaging and toxic to cells. The results showed, visible mitodepressive action of 4.02 mg/L 2,4-D when treated for 48 h that had tendency to become toxic if the roots had been in touch with 2,4-D for a longer time.

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“…The results are in clear agreement with observations of Mohandas and Grant (1972); Tomkins and Grant (1976), Prasad and Das (1977) and Kumari and Vaidyanath (1989).…”

Section: Resultssupporting

confidence: 92%

Does 2,4-dichlorophenoxyacetic acid (2,4-D) induce genotoxic effects in tissue cultured Allium roots?

et al. 2016

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“…The occurrence of sticky bridge in GA-treated roots has been reported in A. cepa and A. fistulosum (Kato 1955). In V. faba, however, stickiness is absent and Prasad and Das (1977) are of opinion that GA-induced bridges are probably dicentric chromatid bridges.…”

Section: Discussionmentioning

confidence: 90%

“…In V. faba, however, low concentra tion of GA enhances whereas high concentration inhibits cell division (Prasad and Das 1977).…”

Section: Discussionmentioning

confidence: 95%

“…

Material and methodsRoot meristem of Allium cepa, grown from bulbs, formed the material of the present investigation.D etermination of mitotic index Mitotic index (MI) was determined in control and treatments with 84 and 126 R x-rays, 25ppm of indole acetic acid (IAA), 50ppm of gibberellic acid (GA3) singly and in combination of x-rays and growth hormones.Root tips from roots of suitable length of control and treated materials were fixed in a mixture of propionic acid and ethyl alcohol (1:3v/v) 1, 3, 6, 9, 12 and 24hr after treatment.Temporary squash preparations were made using propionic orcein as the stain. Cells with mitotic stages from prophase to telophase were scored against total number of cells observed in microscopic fields from coded slides ar ranged randomly.

Modification of x-ray-induced chromosomal damage Root meristems were treated with different concentrations of IAA and GA3 before and after treatments with 84 and 126 R x-rays.

…”

mentioning

confidence: 99%

“…Plant growth hormones have been known to influence mitotic index value (Kato 1955, McManus 1960, Humphries and Wheeler 1960, Davidson and MacLeod 1966, Prasad and Das 1977 and to modify radiation-induced chromosomal damage (Guttman and Brown 1959, Magdon 1964, Araratyan and Azatyan 1974, Araratyan and Vardanyan 1976.…”

mentioning

confidence: 99%

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Cytological effects of single and combined treatments with X-rays, IAA and GA3 in Allium cepa.

Mandal

Basu

1981

CYTOLOGIA

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Plant growth hormones have been known to influence mitotic index value (Kato 1955, McManus 1960, Humphries and Wheeler 1960, Davidson and MacLeod 1966, Prasad and Das 1977 and to modify radiation-induced chromosomal damage (Guttman and Brown 1959, Magdon 1964, Araratyan and Azatyan 1974, Araratyan and Vardanyan 1976.The effects of x-rays singly and in combination with IAA and GA3 on mitotic index and modification of chromosomal damage in root meristem of Allium cepa by pre and post-irradiation treatments with these growth hormones have been reported. Material and methodsRoot meristem of Allium cepa, grown from bulbs, formed the material of the present investigation.D etermination of mitotic index Mitotic index (MI) was determined in control and treatments with 84 and 126 R x-rays, 25ppm of indole acetic acid (IAA), 50ppm of gibberellic acid (GA3) singly and in combination of x-rays and growth hormones.Root tips from roots of suitable length of control and treated materials were fixed in a mixture of propionic acid and ethyl alcohol (1:3v/v) 1, 3, 6, 9, 12 and 24hr after treatment.Temporary squash preparations were made using propionic orcein as the stain. Cells with mitotic stages from prophase to telophase were scored against total number of cells observed in microscopic fields from coded slides ar ranged randomly.Modification of x-ray-induced chromosomal damage Root meristems were treated with different concentrations of IAA and GA3 before and after treatments with 84 and 126 R x-rays. The materials kept in dis tilled water served as control.Pre-irradiation treatment: Growing roots were placed in aqueous solutions of 25, 50 and 100ppm IAAA and 50, 100 and 200ppm GA3, and distilled water (con trol) in such a way that only the root tips were immersed in them for 24hr. After the treatments the roots were thoroughly washed in water. The superficial water was wiped off and the roots were irradiated with 84 and 126 R x-rays. The roots were again kept immersed in water for 24hr before fixation.

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Salt stress affects mitotic activity and modulates antioxidant systems in onion roots

Singh

Roy

2015

Braz. J. Bot

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Effects of Some Growth Substances on Mitosis

Cited by 13 publications

References 9 publications

Does 2,4-dichlorophenoxyacetic acid (2,4-D) induce genotoxic effects in tissue cultured Allium roots?

Does 2,4-dichlorophenoxyacetic acid (2,4-D) induce genotoxic effects in tissue cultured Allium roots?

Cytological effects of single and combined treatments with X-rays, IAA and GA3 in Allium cepa.

Salt stress affects mitotic activity and modulates antioxidant systems in onion roots

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