Effects of solvent type on phenolics and flavonoids content and antioxidant activities in Onosma dichroanthum Boiss.

Mazandarani, M

doi:10.5897/jmpr11.1460

Cited by 24 publications

(23 citation statements)

References 38 publications

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“…Previous investigations have demonstrated that alteration in solvent polarity may be used to differentially precipitate selected antioxidant compounds [1, 61]. In the present study, significant differences in free radical scavenging ability for extracts of Phoradendron californicum -mesquite were observed dependent on the kind of extraction solvent used, while in Phoradendron californicum -oak the effect of solvent was minimized.…”

Section: Resultsmentioning

confidence: 45%

“…Free radicals contribute to more than one hundred disorders in humans, including atherosclerosis, arthritis, ischemia and repercussion injury of many tissues, central nervous system injury, gastritis, and cancer [1]. Environmental pollutants, including radiation, chemicals, and dietary toxicants, along with physical trauma, cause dysregulation of immune activity, and may alter gene expression to induce expression of abnormal proteins.…”

Section: Introductionmentioning

confidence: 99%

“…Current isolation and chemical purification methods used include solvent extraction processes that utilize solvent polarity as a major separation technique. These methods frequently include the use of ethyl acetate, phenol/chloroform, aqueous, and several other approaches [1, 9, 11–15]. …”

Section: Introductionmentioning

confidence: 99%

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Solvent effects on phytochemical constituent profiles and antioxidant activities, using four different extraction formulations for analysis of Bucida buceras L. and Phoradendron californicum

Assanga¹,

Lewis-Luján²,

et al. 2015

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BackgroundThe present investigation evaluated 4 different solvent compositions for their relative capacity to extract total phenolic and total flavonoid (TF) components of the leaves, trunks, and stems of Bucida buceras L. (Combretaceae), and the stems of Phoradendron californicum (Viscaceae), plus mesquite and oak species endemic to the Southwestern United States, northern Mexico, and tropical regions of Central and South America, as well as to profile the composition of these plant materials and to measure their antioxidant capacity.MethodsThe total phenolic content of plant material used in the present investigation was measured using the Folin–Ciocalteau assay. Total flavonoids were assayed by AlCl3 and 2,4-dinitrophenylhydrazin colorimetry. Nitroblue tetrazolium was utilized for scavenging of superoxide anion, and in vitro antioxidant activity was evaluated using the 2, 2-diphenyl-1-picrylhydrazyl and Ferric Reducing/Antioxidant Power assays.ResultsPhytochemical screening of each plant extract evaluated revealed the following major results: (1) No evidence of alkaloids for each of the extraction phases tested was detected in the hexanic, ethanolic, or aqueous phases of Bucida buceras and Phoradendron californicum (oak and mesquite); (2) Analysis of the hexane phase of B. buceras and P. californicum (mesquite) extracts revealed the presence of carotenes, triterpenes/steroids, and lactonic groups; (3) Analysis of the ethanol and aqueous extraction phases for both plants revealed the presence of a diverse range of compounds, including tripterpenes/steroids, lactonics groups, saponins, phenols/tannins, amines and/or amino acids, and flavonoids/anthocyanins; and (4) The highest total phenolic and flavonoid content were observed in P. californicum (oak): 523.886 ± 51.457 µg GAE/mg extract and 409.651 ± 23.091 µg/mg of extract for methanol and aqueous fractions, respectively. The highest flavonoid content was 237.273 ± 21.250 µg PNE/mg extract in the acetone extract of Bucida buceras stems; while the flavonol content (260.685 ± 23.031 µg CE/mg extract) was higher in the ethanol extract of P. californicum (oak). The acetone extract of B. buceras trunk extract showed the highest levels of DPPH radical-scavenging activity (IC50 = 4.136 ± 0.446 µg/mL) and reducing power (4928.392 ± 281.427 µM AAE/mg extract). The highest superoxide radical scavenging activity (IC50) was 55.249 ± 9.829 µg/mL, observed in acetone extracts of B. buceras leaves.ConclusionsThe results of the present investigation demonstrated the effects of extraction solvent on phenolic and flavonoid content yield—and antioxidant activities by Bucida buceras and Phoradendron californicum. The present investigation further revealed that Bucida buceras exhibited optimal antioxidant capacity when acetone was used as extraction solvent; and the highest yield of phenols and flavonoids were obtained from the P. californicum oak, using methanol and aqueous solvents, respectively.

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Section: Resultsmentioning

confidence: 45%

Section: Introductionmentioning

confidence: 99%

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Solvent effects on phytochemical constituent profiles and antioxidant activities, using four different extraction formulations for analysis of Bucida buceras L. and Phoradendron californicum

Assanga¹,

Lewis-Luján²,

et al. 2015

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“…It has been reported that dehydrated LBBs had the TPC value of 351 ± 7.25 mgGAE/100 g, performing the extraction with 80% methanol [33]. The influence of the solvent, used for the extraction on the TPC value, has been previously investigated and it was found a reduction of 1.3-fold or 2.2-fold using methanol instead of ethanol [34, 35]. In any case, also taking into account the variability due to different method of extraction, the Umbrian LBBs have a TPC higher than commercial one.…”

Section: Resultsmentioning

confidence: 99%

In VitroProtective Effects ofLycium barbarumBerries Cultivated in Umbria (Italy) on Human Hepatocellular Carcinoma Cells

Ceccarini

Vannini

Cataldi

et al. 2016

BioMed Research International

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Lycium barbarum is a famous plant in the traditional Chinese medicine. The plant is known to have health-promoting bioactive components. The properties of Lycium barbarum berries cultivated in Umbria (Italy) and their effect on human hepatocellular carcinoma cells (HepG2) have been investigated in this work. The obtained results demonstrated that the Lycium barbarum berries from Umbria region display high antioxidant properties evaluated by total phenolic content and ORAC method, on hydrophilic and lipophilic fractions. Moreover, on HepG2 cell line Lycium barbarum berries extract did not change cell viability analyzed by MTT and Trypan blue exclusion assay and did not induce genotoxic effect analyzed by comet assay. Furthermore, it was demonstrated, for the first time, that the berries extract showed a protective effect on DNA damage, expressed as antigenotoxic activity in vitro. Finally, Lycium barbarum berries extract was able to modulate the expression of genes involved in oxidative stress, proliferation, apoptosis, and cancer. In particular, downexpression of genes involved in tumor migration and invasion (CCL5), in increased risk of metastasis and antiapoptotic signal (DUSP1), and in carcinogenesis (GPx-3 and PTGS1), together with overexpression of tumor suppressor gene (MT3), suggested that Umbrian Lycium barbarum berries could play a protective role against hepatocellular carcinoma.

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“…Usually, aqueous solutions containing organic solvent ranging from 50 to 80%, tend to modulate the polarity of the solvent mixture (Do et al, 2014) and the variety of polyphenolic constituents, unlike a pure aqueous solvent (Mazandarani et al, 2012). This may explain the higher values for both total flavonoids and total phenolics (p<0.05) found for EE (1.89 mg QE/g and 0.52 g GAE/g) and AcE (2.13 mg QE/g and 0.51 g GAE/g) compared to AE (1.35 mg QE/g and 0.35 g GAE/g) ( Table 1).…”

Section: Resultsmentioning

confidence: 99%

Stem bark extracts of Endopleura uchi (Huber) Cuatrec: Inhibition of pancreatic lipase and antioxidant activity

Gustavo¹,

Anderson²,

Luciana³

et al. 2017

J. Med. Plants Res.

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Pancreatic lipase is considered an important target for the control of hyperlipidemia. Several plants, especially those rich in phenolic metabolites, have been shown to have anti-hyperlipidemic activity and are considered a good alternative for obesity prevention. Extracts of the stem bark of Endopleura uchi (Huber) Cuatrec (Humiriaceae) were evaluated for the inhibitory activity on the pancreatic lipase enzyme, as well as their antioxidant potential were verified in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) test. In addition, the total phenolics (TPC) and flavonoids contents (TFC) were estimated. In general way, the acetonic and ethanolic extracts showed better results than aqueous extract. At the concentration of 1 mg/mL, both acetonic and ethanolic extracts inhibited the activity of pancreatic lipase by 49.33 and 36.88%, representing 135.26 and 102.75 of inhibited lipase activity per gram of extract (ILA/g). On the other hand, the aqueous extract inhibited lipase by 47.54% at the concentration of 2 mg/mL, which means 213.84 ILA/g. The highest antioxidant activity was observed for the acetonic extract with a 50% effective concentration (EC 50 ) of 7.9 µg/mL, followed by the ethanolic extract with EC 50 of 9.7 µg/mL and the aqueous extract with EC 50 of 12.4 µg/mL. TPC in gallic acid equivalent per gram of sample (GAE/g) were 0.52, 0.51 and 0.35 respectively for the ethanolic, acetonic and aqueous extracts. In turn, TFC in quercetin equivalent per gram of sample (QE/g) were 2.13, 1.89 and 1.35 for the acetonic, ethanolic and aqueous extracts, respectively. Positive and strong correlations (r Pearson > 9.0) between TPC and antioxidant activity were found for all 3 extracts. These results suggest that both pancreatic lipase inhibition and antioxidant activity were distinguished by organic solvents and water extraction. Furthermore, organic extracts (acetone and ethanol) showed to be richer in phenolic metabolites. These metabolites may be related to the biological activities that were found, indicating the stem bark extracts of E. uchi as possible candidates for the development of strategies in the prevention of obesity and hyperlipidemia.

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Effects of solvent type on phenolics and flavonoids content and antioxidant activities in Onosma dichroanthum Boiss.

Cited by 24 publications

References 38 publications

Solvent effects on phytochemical constituent profiles and antioxidant activities, using four different extraction formulations for analysis of Bucida buceras L. and Phoradendron californicum

Solvent effects on phytochemical constituent profiles and antioxidant activities, using four different extraction formulations for analysis of Bucida buceras L. and Phoradendron californicum

In VitroProtective Effects ofLycium barbarumBerries Cultivated in Umbria (Italy) on Human Hepatocellular Carcinoma Cells

Stem bark extracts of Endopleura uchi (Huber) Cuatrec: Inhibition of pancreatic lipase and antioxidant activity

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