Effects of soil pH on rhizoctonia damping-off of sugar beet and disease suppression induced by soil amendment with crop residues

Watanabe, Kazufumi; Matsui, Mariko; Honjo, Hitoshi; Becker, J. Ole; Fukui, Ryo

doi:10.1007/s11104-011-0843-6

Cited by 23 publications

(11 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Reflecting on this study, the differences in the soil environmental factors between the diseased and RSD-treated soils, such as the pH and carbon quality and quantity, may have been large enough to exceed the adaptation ranges of the suppressive agents that may have ultimately induced the nontransferability of the disease suppression capability. This interpretation is further supported by the fact that disease suppression markedly declined when the abiotic environment, such as the pH, of the disease-suppressive soil changed greatly (33, 34). Additionally, we also found that many microbial species, such as Zopfiella and Sphingobacterium , were highly influenced by reinoculated microbiota, where their relative abundances in the reestablished soil was associated with their initial abundances in the inoculants.…”

Section: Discussionmentioning

confidence: 88%

Characterizing the Key Agents in a Disease-Suppressed Soil Managed by Reductive Soil Disinfestation

Liu

Huang

Zhao

et al. 2019

Appl Environ Microbiol

View full text Add to dashboard Cite

Many agricultural soil management strategies have been shown to be effective in preventing soilborne diseases. However, their underlying mechanisms of action remain unknown. In this study, we used reductive soil disinfestation (RSD), also named anaerobic soil disinfestation (ASD) and biological soil disinfestation (BSD), as a representative method for disease management and cucumber damping-off diseased soil as a model system to identify the disease-suppressive agents in artificially managed soil. The results showed that RSD created a soil environment that was different from that of the diseased soil, where the pH level and the carbon content were greater. Heat treatment and pathogen or soil microbiota self- and cross-reinoculations resulted in the expansion of various soil microbial communities harbored by the two soil environments, as well as various disease incidences. Environmental factors were the primary determinant of the reassembled bacterial community, followed by initial microbiota, whereas initial microbiota was the key driver of the reassembled fungal community. The relative abundances of the bacterial order Sphingobacteriales and fungal order Sordariales, as well as their affiliated genera Sphingobacterium, unclassified genus within Sphingobacteriaceae, Zopfiella, and unclassified genera within Lasiosphaeriaceae and Chaetomiaceae, were negatively correlated with disease incidence and positively associated with RSD-conditioned soil environment. Furthermore, we validated that both the microbial disease-suppressive agent and its adapted abiotic environment contributed to disease suppression. Our results elucidate the abiotic and biotic foundations of soilborne disease suppression under artificial management and highlight that the abiotic environment is as important as the microbial agents in disease suppression. IMPORTANCE Most defined systems have identified microbial elements as the primary factors determining disease suppression, but the involvement of the soil abiotic environment is less defined. The significance of this work is that the soil abiotic environment plays a critical role in the establishment of the soil microbial community and key microbial agents that directly contribute to the prevention of soilborne diseases. We highlight the importance of the soil abiotic environment in disease suppression. Furthermore, we provide a framework for the characterization of disease-suppressing agents in artificially managed soil. These results will gradually close the gap in knowledge on soil environment-microbe interactions.

show abstract

Section: Discussionmentioning

confidence: 88%

Characterizing the Key Agents in a Disease-Suppressed Soil Managed by Reductive Soil Disinfestation

Liu

Huang

Zhao

et al. 2019

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…However, we only used pasteurized soil to test the virulence of the isolates. The virulence of pathogens such as multinucleate R. solani , and the activity of antagonistic soil microorganisms on suppressing disease are influenced by soil media [47], [48]. For this reason, it is possible that the observed virulence levels may have been somewhat elevated compared to those in non-pasteurized soil.…”

Section: Discussionmentioning

confidence: 99%

Wide Variation in Virulence and Genetic Diversity of Binucleate Rhizoctonia Isolates Associated with Root Rot of Strawberry in Western Australia

2013

View full text Add to dashboard Cite

Strawberry (Fragaria×ananassa) is one of the most important berry crops in the world. Root rot of strawberry caused by Rhizoctonia spp. is a serious threat to commercial strawberry production worldwide. However, there is no information on the genetic diversity and phylogenetic status of Rhizoctonia spp. associated with root rot of strawberry in Australia. To address this, a total of 96 Rhizoctonia spp. isolates recovered from diseased strawberry plants in Western Australia were characterized for their nuclear condition, virulence, genetic diversity and phylogenetic status. All the isolates were found to be binucleate Rhizoctonia (BNR). Sixty-five of the 96 BNR isolates were pathogenic on strawberry, but with wide variation in virulence, with 25 isolates having high virulence. Sequence analysis of the internal transcribed spacers of the ribosomal DNA separated the 65 pathogenic BNR isolates into six distinct clades. The sequence analysis also separated reference BNR isolates from strawberry or other crops across the world into clades that correspond to their respective anastomosis group (AG). Some of the pathogenic BNR isolates from this study were embedded in the clades for AG-A, AG-K and AG-I, while other isolates formed clades that were sister to the clades specific for AG-G, AG-B, AG-I and AG-C. There was no significant association between genetic diversity and virulence of these BNR isolates. This study demonstrates that pathogenic BNR isolates associated with root rot of strawberry in Western Australia have wide genetic diversity, and highlights new genetic groups not previously found to be associated with root rot of strawberry in the world (e.g., AG-B) or in Australia (e.g., AG-G). The wide variation in virulence and genetic diversity identified in this study will be of high value for strawberry breeding programs in selecting, developing and deploying new cultivars with resistance to these multi-genetic groups of BNR.

show abstract

“…In the present study small increases in soil pH of 0.2 to 0.4 units was observed when B. carinata pellets were used. Increased suppressiveness of Rhizoctonia damping-off of sugar beet in a near neutral to alkaline soil, compared to acid soil, from use of dried peanut plant residues, has been reported, due to increased activity of specific antagonistic soil microorganisms (Watanabe et al, 2011). Similar disease suppression effects have been obtained by amending soils with composts rich in cellulolytic and oligotrophic actinomycete antagonists (Tuitert et al, 1998;Kasuya et al, 2006;Ros et al, 2006).…”

Section: Carbon Sourcementioning

confidence: 72%

Evaluation of different carbon sources for anaerobic soil disinfestation against Rhizoctonia solani on lettuce in controlled production systems

Gilardi¹,

Pugliese²,

Gullino³

et al. 2020

Phytopathol. Mediterr.

View full text Add to dashboard Cite

Effects of anaerobic soil disinfestation (ASD) on Rhizoctonia solani basal rot of lettuce were assessed considering: two soil types; different C-sources; different temperature regimes; two treatment durations; and two lettuce crop cycles, in the presence of a high disease incidence from artificial infestation with the pathogen. C-source, temperature, and incubation period, and their interaction, affected the efficacy of the ASD treatment for the lettuce–R. solani pathosystem, with differences depending on the soil type. Brassica carinata pellets, used as a C-source, reduced incidence of Rhizoctonia basal rot in the first crop cycle by 50 to 69% in a peat soil after 3 weeks of treatment at 21°C, and by 52 to 60% after 3 weeks of treatment at 26 or 31°C, compared to the inoculated and untreated experimental controls without anaerobic conditions. The best disease reduction was provided by B. carinata pellets applied, under anaerobic conditions, to peat soil (79% efficacy) and a sandy loam soil (100% efficacy) kept at 31°C for 6 weeks. Generally, ASD based on B. carinata pellets provided greater disease reduction in the first crop cycle than the second, with the only exception being for results achieved in both soils incubated for 6 weeks at 21, 26 and 31°C. Wild rocket used as a C-source provided the greatest disease reduction (78–83%) on plants grown in peat soil at the first crop cycle after 6 weeks of the ASD treatment at 31°C, while wild rocket provided disease reduction of 29 and 50% when mixed with the sandy-loam soil under the same conditions for 6 weeks. The efficacy of the ASD treatment with compost was improved in the second crop cycle, compared to the first, resulting in the greatest disease reduction (52 and 66% efficacy) reached in the sandy-loam soil incubated for 3 weeks at 21°C, and 63% efficacy in peat soil previously treated for 6 weeks at 26 and 31°C. However, slight reductions in lettuce plant development was generally evident in the first crop cycle, which could be due to differences in efficacy of the tested ASD treatments and to phytotoxicity. The greatest yield from sandy loam soil was obtained for the B. carinata pellets and wild rocket at 26 and 31°C in the second cultivation cycle. Partial efficacy of ASD does not justify the adoption of this method against R. solani on lettuce under intensive crop systems. However, ASD based on Brassicaceae and compost as carbon source applied in a sandy-loam soil may be valuable for reducing R. solani incidence, at lower temperatures than those required for soil solarisation or biosolarisation.

show abstract

Effects of soil pH on rhizoctonia damping-off of sugar beet and disease suppression induced by soil amendment with crop residues

Cited by 23 publications

References 24 publications

Characterizing the Key Agents in a Disease-Suppressed Soil Managed by Reductive Soil Disinfestation

Characterizing the Key Agents in a Disease-Suppressed Soil Managed by Reductive Soil Disinfestation

Wide Variation in Virulence and Genetic Diversity of Binucleate Rhizoctonia Isolates Associated with Root Rot of Strawberry in Western Australia

Evaluation of different carbon sources for anaerobic soil disinfestation against Rhizoctonia solani on lettuce in controlled production systems

Contact Info

Product

Resources

About