Effects of sodium β-sitosteryl sulfate on the phase behavior of dipalmitoylphosphatidylcholine

Effects of <i>β</i>-Sitosteryl Sulfate on the Phase Behavior and Hydration Properties of Distearoylphosphatidylcholine: a Comparison with Dipalmitoylphosphatidylcholine

“…The broad component representing the L o L α transition in this case is located on the left side black arrow of the sharp component. These thermograms show that the sharp component is affected to a lesser extent than the analogous component in the case of DPPC 18 , while the broad component black arrows is still strongly affected. The unequal influence of PSO 4 on the two components of the DSC peak leads to a clear separation of these two components.…”

“…Only temperatures representing the overall transition of the coexisting lamellar phases L o and L ß into the L α phase were considered in this simplified 43,44 phase diagram, instead of including the temperatures corresponding to the individual peak components. The phase diagram for DPPC-PSO 4 from our previous study 18 has also been presented in Fig. 6 for the sake of comparison.…”

“…Finally, the solution was filtered, and PSO 4 was obtained from the filtrate by removing the methanol in a rotary evaporator at 70 . The resulting sterol isolated from the non-sulfate impurities by this method was used for the study 18 . Fig.…”

“…A cyclone reader PerkinElmer Inc., Massachusetts, USA was used to read the scattering patterns, which were converted to 1D profiles using SAXSquant software Anton Paar Co., Ltd. . All data were normalized to the same incident primary beam intensity 18,23 .…”

“…Despite these merits, no sufficient studies have been performed so far on the interaction between phytosterol sulfates and phosphatidylcholines. Previously, we carried out the first such study on DPPC-PSO 4 mixtures 18 . In the present paper, we discuss the phase behavior and hydration properties of DSPC in the presence of PSO 4 and compare them with the results for DPPC.…”