Effects of &lt;i&gt;β&lt;/i&gt;-Sitosteryl Sulfate on the Phase Behavior and Hydration Properties of Distearoylphosphatidylcholine: a Comparison with Dipalmitoylphosphatidylcholine

Kafle, Ananda; Misono, Takeshi; Bhadani, Avinash; Akamatsu, Masaaki; Sakai, Kenichi; Kaise, Chihiro; Kaneko, Teruhisa; Sakai, Hideki

doi:10.5650/jos.ess17182

Cited by 6 publications

(13 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A similar effect of the enhanced hydration has been reported previously by Mohapatra and Mishra 20 in DPPC membranes containing bile salt. This enhancement of hydration of DPPC by PSO 4 is also consistent with our previous reports 8,9 . For the sonicated samples, a comparable tendency was observed data not shown but the r value was very low; this could be due to poor incorporation of the probe in the bilayer because of the extremely small sizes of the liposomes and their high curvature.…”

Section: Dph Uorescence Anisotropysupporting

confidence: 93%

Effects of β-Sitosteryl Sulfate on the Properties of DPPC Liposomes

et al. 2018

Self Cite

View full text Add to dashboard Cite

The effect of β-sitosteryl sulfate (PSO 4) on the liposomal size, stability, fluidity, and dispersibility of DPPC liposomes prepared by vortex mixing, bath-sonication, and probe-sonication has been studied. PSO 4 significantly decreases the particle size of the multilamellar liposomes (MLVs). The sizes of the vortexmixed and the bath-sonicated liposomes vary as a function of PSO 4 concentration. On the other hand, PSO 4 has only little effect on the particle sizes of probe sonicated liposomes. In some cases, the liposomal stability at higher PSO 4 concentrations depends on the preparation method. PSO 4 improves the dispersibility of the DPPC liposomes and enhances their hydration. It also increases the fluidity of the liposomes prepared by each method. Our results suggest that liposomes consisting of DPPC and PSO 4 can be suitable as a cosmetic or pharmaceutical ingredient for the effective delivery of the active components into the body.

show abstract

Section: Dph Uorescence Anisotropysupporting

confidence: 93%

Effects of β-Sitosteryl Sulfate on the Properties of DPPC Liposomes

et al. 2018

Self Cite

View full text Add to dashboard Cite

show abstract

“…This can also be justified from our previous studies on pure phospholipids that exhibit highly cooperative phase transitions. 31,34 The occurrence of two gel to liquid crystal phase transition temperatures can be attributed to the nonideal mixing of the higher-melting PE/PA components in the lowermelting PC fraction of lecithin. The phenomenon of nonideal mixing of lipids has been described by previous researchers including Inoue et al 35 and Garidel et al, 36 who studied this phenomenon in the PC−PE and PC−PA mixtures, respectively.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…This effect of PSO 4 on soy lecithins is comparable to its effect on pure DPPC. 31,34 In the case of high-PC-HLC, the abolition of the T m1 peak occurred at 0.3 mole fraction of PSO 4 . These observations provide further evidence for the assignment of the T m1 peak to the phase transition of the PC fraction of the HLC sample.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…2,24 The L o phase is a fluid phase induced by a steroid and possesses characteristics intermediate between the gel and liquid crystalline (L α ) phases. PSO 4 , at a significantly high concentration, converts a PC from gel into the fluid phase, 31,34 thus abolishing the T m peak. Unlike PCs, however, a similar abolition of the T m peaks of PE and PA by a sterol does not occur.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…The miscibility of PSO 4 in HLC is limited to below 30 mol % as compared to above 50 mol % in DPPC and above 40 mol % in DSPC. 31,34 This poor miscibility of PSO 4 in HLC compared to pure PCs can be attributed to its lower PC content in the mixture that also contains PE and PA. Binary Phase Diagram. As obvious from the above discussion, below the main phase transition temperatures, the samples ranging in x from 0 to 0.1 exhibit two gel phases labeled L β1 and L β2 .…”

Section: ■ Results and Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Phase Behavior of the Bilayers Containing Hydrogenated Soy Lecithin and β-Sitosteryl Sulfate

et al. 2020

Self Cite

View full text Add to dashboard Cite

The phase behaviors of systems containing saturated phosphatidylcholine (PC) and plant steroids can be important for designing new alternative delivery methods. In our previous studies, we found that even a small amount of β-sitosteryl sulfate (PSO4) significantly affects the phase behavior, hydration properties, and liposomal properties of pure saturated phosphatidylcholines [KafleA. Kafle, A. Colloids Surf., B20181615966 KafleA. Kafle, A. J. Oleo Sci.20186715111519]. In the current paper, we are reporting the phase behavior of a more complex system consisting of hydrogenated soy lecithin (HLC), which is useful as a carrier in drug delivery systems or in cosmetics, and PSO4. HLC, which is composed of phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidic acid (PA), and lysophosphatidylcholine (LPC), demonstrated a versatile phase behavior. The PC component of HLC was found to separate from the PE and PA components as a result of nonideal mixing. At room temperature, these two domains represented two distinct gel phases denoted Lβ1 and Lβ2. The Lβ1 phase selectively underwent transition into the liquid crystalline phase (Lα) at a lower temperature than Lβ2. Upon addition of PSO4, at room temperature, the PC fraction gradually converted into the liquid-ordered (Lo) phase, while the (PE + PA) fraction remained unaffected. When heated above 60 °C, the whole material converted into the liquid crystalline phase. The observed fluidizing effect of PSO4 on HLC can find applications in preparing vehicles for moisture or drugs in cosmetic and pharmaceutical formulations.

show abstract