Effects of six diterpenes on macrophage eicosanoid biosynthesis

Heras, Beatriz de las; Abad, María J. F.; Silván, A. M.; Pascual, Raquel García; Bermejo, Paulina; Rodrı́guez, Benjamı́n; Villar, Ángel

doi:10.1016/s0024-3205(01)01402-3

Cited by 35 publications

(10 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although diterpenoids have been reported to be anti-inflammatory by inhibiting both 5-lipoxygenase and cyclo-oxygenase, it remains to be determined whether crotonin and crotofoline A are responsible for this activity in our assay [25] . Furthermore, it has been reported that the diterpenoids present in C. sylvaticus cause irritation to the skin.…”

Section: Discussionmentioning

confidence: 99%

In vitro 5-Lipoxygenase and Anti-Oxidant Activities of South African Medicinal Plants Commonly Used Topically for Skin Diseases

Frum

Viljoen

2006

Skin Pharmacol Physiol

View full text Add to dashboard Cite

An investigation was undertaken to determine the possible mechanisms of action of medicinal plants used for dermatological pathologies. A total of 14 plant species were selected from the readily available ethnobotanical literature. 5-Lipoxygenase and DPPH (2,2-diphenyl-1-picrylhydrazyl) assays were used to determine the anti-inflammatory activity and the anti-oxidant activity of selected medicinal plants, respectively. Both aqueous and methanol extracts were tested. Among the plants screened, four species (Croton sylvaticus, Warburgia salutaris, Pentanisia prunelloides, and Melianthus comosus) displayed promising 5-lipoxygenase inhibitory activity with IC₅₀ values <61 ppm. A large number of plants exhibited significant anti-oxidant activities with IC₅₀ values between 5.27 and 83.36 ppm. Aqueous extracts of M. comosus exhibited the most potent anti-inflammatory and anti-oxidant activity.

show abstract

Section: Discussionmentioning

confidence: 99%

In vitro 5-Lipoxygenase and Anti-Oxidant Activities of South African Medicinal Plants Commonly Used Topically for Skin Diseases

Frum

Viljoen

2006

Skin Pharmacol Physiol

View full text Add to dashboard Cite

show abstract

“…NO synthesized by inducible nitric oxide synthesis (iNOS) has been reported as a major inflammatory mediator and seems to be involved in both acute and chronic inflammation [22]. The physiological or normal generation of NO mediates the bactericidal and tumoricidal actions of macrophages.…”

Section: Discussionmentioning

confidence: 99%

The Anti-inflammatory Effects of Water Extract fromCordyceps militarisin Murine Macrophage

Choi

Kim

et al. 2010

Mycobiology

View full text Add to dashboard Cite

The aim of this study was to determine the in vitro anti-inflammatory effect of hot water extract from Cordyceps militaris fruiting bodies (CMWE) on lipopolysaccharide (LPS)-stimulated nitric oxide (NO) production, tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) release in RAW 264.7 cells. The treatment of macrophages with various concentrations of hot CMWE significantly reduced LPS-induced production as well as NO, TNF-α and IL-6 secretion in a concentration-dependent manner. These results suggest that CMWE have potent inhibitory effects on the production of these inflammatory mediators.

show abstract

“…Cytotoxicity studies were performed by the mitocohondrial‐dependent reduction of the tetrazolium salt 3(4,5‐dimethylthiazol‐2yl)‐2,5‐diphenyltetrazolium bromide) to formazan (MTT method). Peritoneal macrophages were obtained from Swiss male mice according to the technique previously described 22. Briefly, macrophages were diluted in Dulbecco's Modified Eagle's medium (DMEM) with 10% heat‐inactivated fetal calf serum, and antibiotics (streptomycin 100 μg/mL and 100 U penicillin/mL) were pipetted onto 96‐well microtiter plates and incubated overnight at 37°C in a 5% CO 2 /95% O 2 atmosphere.…”

Section: Methodsmentioning

confidence: 99%

Biocompatibility of elastin‐like polymer poly(VPAVG) microparticles: in vitro and in vivo studies

Rincón

Molina‐Martínez

Heras

et al. 2006

J Biomedical Materials Res

Self Cite

View full text Add to dashboard Cite

Poly(L-valine-L-proline-L-alanine-L-valine-L-glycine) (VPAVG) is a new kind of proteinaceous polymer belonging to the Elastin-like family. These polymers are based on the recurrence of certain short peptide monomers that are considered as "building blocks" in the natural elastin. This smart thermoresponsive polymer has the ability to self-associate at physiological temperature to form aggregates with about 60% in water. This ability can be harnessed to prepare microparticles loaded with an active substance. The aim of this report is to evaluate, from the results of the experiment conducted, the biocompatibility of microparticles prepared from poly(VPAVG). We have studied the cytotoxic effects of microparticles, edema formation after subcutaneous injection (1 and 2.5 mg) in rats (n = 6), and also intraocular tolerance after the intravitreal injection of 2.5 mg of poly(VPAVG) microparticles into pigmented rabbits (n = 12). The polymer did not induce any cytotoxicity or nonspecific depression of cellular respiration on macrophages under the range of polymer concentrations investigated in this study (20, 30, 40, and 60 mg/mL). We observed no inflammatory response to microparticles after subcutaneous injection in the hind-paw of rats, with no significant differences between the control group (PBS) and experimental groups. Anterior and posterior segment signs were evaluated after intraocular injection of poly(VPAVG) microparticles. Only a few eyes (2/11) of the experimental group presented inflammation signs at day 28 postinjection. Nevertheless, 45% (5/11) of the eyes receiving microparticles showed tractional retinal detachment. The results observed in this work suggested certain fibroblastic activity induced by poly(VPAVG) microparticles after their intraocular injection.

show abstract

Effects of six diterpenes on macrophage eicosanoid biosynthesis

Cited by 35 publications

References 0 publications

In vitro 5-Lipoxygenase and Anti-Oxidant Activities of South African Medicinal Plants Commonly Used Topically for Skin Diseases

In vitro 5-Lipoxygenase and Anti-Oxidant Activities of South African Medicinal Plants Commonly Used Topically for Skin Diseases

The Anti-inflammatory Effects of Water Extract fromCordyceps militarisin Murine Macrophage

Biocompatibility of elastin‐like polymer poly(VPAVG) microparticles: in vitro and in vivo studies

Contact Info

Product

Resources

About