Effects of SiO2 nanoparticles on phospholipid membrane integrity and fluidity

Wei, Xiaoran; Jiang, Wei; Yu, Junchao; Ding, Lei; Hu, Jingtian; Jiang, Guibin

doi:10.1016/j.jhazmat.2015.01.063

Cited by 55 publications

(31 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, it seems that the NP-SLB interaction is mainly driven by the high surface energy of the bare NPs that tend to extract lipids from the bilayer. 16,57 Montis and co-workers 58 Although the HC constitutes the most permanent protein shell on the NP surface and it is considered the biological identity of the NP, a soft corona is also associated in vivo to the NPs and its role in the bio-nano-interaction is still elusive. For this reason, experiments with pure serum and NPs in serum were performed, to investigate if weakly associated and free proteins have a role in the NP-SLB interaction.…”

Section: Discussionmentioning

confidence: 99%

The effect of the protein corona on the interaction between nanoparticles and lipid bilayers

Silvio

Maccarini

Parker

et al. 2017

Journal of Colloid and Interface Science

View full text Add to dashboard Cite

2 HypothesisIt is known that nanoparticles (NPs) in a biological fluid are immediately coated by a protein corona (PC), composed of a hard (strongly bounded) and a soft (loosely associated) layers, which represents the real nano-interface interacting with the cellular membrane in vivo. In this regard, supported lipid bilayers (SLB) have extensively been used as relevant model systems for elucidating the interaction between biomembranes and NPs. Herein we show how the presence of a PC on the NP surface changes the interaction between NPs and lipid bilayers with particular care on the effects induced by the NPs on the bilayer structure. ExperimentsIn the present work we combined Quartz Crystal Microbalance with Dissipation Monitoring (QCM-D) and Neutron Reflectometry (NR) experimental techniques to elucidate how the NPmembrane interaction is modulated by the presence of proteins in the environment and their effect on the lipid bilayer. FindingsOur study showed that the NP-membrane interaction is significantly affected by the presence of proteins and in particular we observed an important role of the soft corona in this phenomenon. KEYWORDSsupported lipid bilayer, protein corona nanoparticles, quartz crystal microbalance, neutron reflectometry, soft corona, hard corona. ABBREVIATIONNPs nanoparticles; SLB supported lipid bilayer; PC protein corona; QCM-D quartz crystal microbalance with dissipation; NR neutron reflectometry; PS polystyrene; PBS phosphate buffered saline; FBS fetal bovine serum; HC hard corona; SC soft corona; DOPC 1,2-Dioleoylsn-glycero-3-phosphocholine; SLD scattering length density; APM area per molecule; 4MW 4 matching water; SMW silicon matching water; LUV large unilamellar vesicle; GUV giant unilamellar vesicle; DPPC Dipalmitoyl-phosphatidyl-choline.3

show abstract

Section: Discussionmentioning

confidence: 99%

The effect of the protein corona on the interaction between nanoparticles and lipid bilayers

Silvio

Maccarini

Parker

et al. 2017

Journal of Colloid and Interface Science

View full text Add to dashboard Cite

show abstract

“…The plasma membrane plays an important role in separating the intracellular and extracellular environments, providing an anisotropic uid phase to support proteins and regulate molecular transport. 23 The integrity of the cellular structure was important to the activities of the bacteria. Thus, SEM observation was carried out to determine whether rhamnolipids carried the risk of membrane damage.…”

Section: Sem Characterizationmentioning

confidence: 99%

Effects of rhamnolipids on the cell surface characteristics of Sphingomonas sp. GY2B and the biodegradation of phenanthrene

Lin

Tong

et al. 2017

RSC Adv.

View full text Add to dashboard Cite

show abstract

“…These aggregated sediments cannot easily be discarded in the same manner as the non-clustered particles (SiC-x and SiC-NH 2 NPs) which remained in the colloid. Thus, SiC-OH NPs may exert an ongoing negative impact on cellular metabolism which may be caused, for example, by the simple mechanical blocking of the membrane due to the quantity of NP clusters, thus resulting in the occurrence of harmful changes in the cellular membrane phase (i.e., gelation) [46]. Since the ζ of the SiC-OH NPs in the RPMI medium supplemented with FBS was -41 mV, the NPs most probably remained in the colloid and exerted no impact on the cellular metabolism.…”

Section: Discussionmentioning

confidence: 99%

Immunomodulatory Potential of Differently-Terminated Ultra-Small Silicon Carbide Nanoparticles

et al. 2020

View full text Add to dashboard Cite

Ultra-small nanoparticles with sizes comparable to those of pores in the cellular membrane possess significant potential for application in the field of biomedicine. Silicon carbide ultra-small nanoparticles with varying surface termination were tested for the biological system represented by different human cells (using a human osteoblastic cell line as the reference system and a monocyte/macrophage cell line as immune cells). The three tested nanoparticle surface terminations resulted in the observation of different effects on cell metabolic activity. These effects were mostly noticeable in cases of monocytic cells, where each type of particle caused a completely different response (‘as-prepared’ particles, i.e., were highly cytotoxic, –OH terminated particles slightly increased the metabolic activity, while –NH2 terminated particles caused an almost doubled metabolic activity) after 24 h of incubation. Subsequently, the release of cytokines from such treated monocytes and their differentiation into activated cells was determined. The results revealed the potential modulation of immune cell behavior following stimulation with particular ultra-small nanoparticles, thus opening up new fields for novel silicon carbide nanoparticle biomedical applications.

show abstract

Effects of SiO2 nanoparticles on phospholipid membrane integrity and fluidity

Cited by 55 publications

References 36 publications

The effect of the protein corona on the interaction between nanoparticles and lipid bilayers

The effect of the protein corona on the interaction between nanoparticles and lipid bilayers

Effects of rhamnolipids on the cell surface characteristics of Sphingomonas sp. GY2B and the biodegradation of phenanthrene

Immunomodulatory Potential of Differently-Terminated Ultra-Small Silicon Carbide Nanoparticles

Contact Info

Product

Resources

About