Effects of Single Exposure of Sodium Fluoride on Lipid Peroxidation and Antioxidant Enzymes in Salivary Glands of Rats

Abstract

Many studies suggest that fluoride exposure can inhibit the activity of various enzymes and can generate free radicals, which interfere with antioxidant defence mechanisms in living systems. To further the understanding of this issue, this present study examined the effects of low-dose fluoride treatment on the activity of enzymatic antioxidant superoxide dismutase (SOD) and catalase (CAT), as well as the levels of lipid peroxidation (LPO) in the parotid (PA) and submandibular (SM) salivary glands of rats. Rats were injected with a single dose of sodium fluoride (NaF) (15 mg F−/kg b.w.) then euthanized at various time intervals up to 24 hours (h) following exposure. NaF exposure did not cause significant differences in SOD or CAT activity or LPO levels in PA glands compared to control. Conversely, SM glands presented increased SOD activity after 3 h and decreased SOD activity after 1, 12, and 24 h, while LPO was increased after 6, 12, and 24 h of the NaF injection. There were no significant differences in the CAT activity in the groups studied. Our results demonstrated that NaF intoxication caused oxidative stress in salivary glands few hours after administration. These changes were more pronounced in SM than in PA gland.

Influence of Dental Restorations on Oxidative Stress in Gingival Crevicular Fluid

“…Accordingly, ANCOVA analysis of the data arranged in respect to the applied restorations showed significant GSH increase by the following restorations listed in descending order: ZPoC > BF > GIC > Amg; and tSOD activity increase by ZPoC > BF > Amg; while MDA decrease was gained by ZPoC > ZPhC > Amg > TEC (Figures 4(a) – 4(f) and 5 ). Consistent with the literature, we confirmed that ZPoC and ZPhC demonstrated profound antioxidant effect in comparison to the other used dental fillings, in terms of suppressed LPO and GSH regaining, contrary to GIC which demonstrated completely opposite, prooxidant effect, while composites, BF and TEC did not show noticeable effects on GCF OS status [ 37 , 38 ].…”

“…The obtained results are consistent with Yamaguti et al's study in which it was shown that low-dose fluoride treatment affects antioxidant enzymes, including SOD and catalase (CAT), and rises LPO in parotid and submandibular salivary glands of rats. Explicitly, they demonstrated that fluoride intoxication caused more pronounced OS in submandibular than in parotid salivary glands [ 38 ].…”

Single oral acute fluoride exposure causes changes in cardiac expression of oxidant and antioxidant enzymes, apoptotic and necrotic markers in male rats

“…In our study, we found decreased cardiac endogenous antioxidants such as SOD2, CAT and GSTpi, GPx and GSH in F -treated rats. In line with this, a previous study showed a similar pattern of antioxidants status in the salivary glands of acute F -intoxicated rats [37].…”

Effects of Krill Euphausia superba Fluoride Extract on Toxicity and Oxidative Stress in Liver cell

“…SOD (Zhang et al, 2000), NaF SOD , CAT , SOD (Yamaguti, 2013). SOD , SOD (Yamaguti, 2013 (Guo et al, 2002). NaF , NaF , CAT, SOD GSH-Px (Trivedi,a et al, 2011).…”