Single oral acute fluoride exposure causes changes in cardiac expression of oxidant and antioxidant enzymes, apoptotic and necrotic markers in male rats

Panneerselvam, Lakshmikanthan; Govindarajan, Vimal; Ameeramja, Jaishabanu; Nair, Harikumaran Raveendran; Perumal, Ekambaram

doi:10.1016/j.biochi.2015.10.002

Cited by 26 publications

(6 citation statements)

References 46 publications

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“…This may be due to the antioxidant property of Sal B which reduces oxidative stress by binding free radicals, thereby reducing cell death (Song et al, 2004). These results are in a consistent with the study of Panneerselvam et al (2015) who reported that Sal B scavenged ROS formation in rats.…”

Section: Discussionsupporting

confidence: 94%

Myocardium Protective Function of Salvianolic Acid B in Ischemia Reperfusion Rats

Qiao

2016

Afr J Tradit Complement Altern Med

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Background: Salvia miltiorrhiza (SM) Bunge is one of the widely-used Chinese medicinal herbs. Salvianolic acid B (Sal B), a bioactive compound isolated from the Chinese herb Radix Salviae Miltiorrhizae, has been reported to exhibit anti-inflammatory and anti-oxidantive effects. Material and method: To study the cardioprotective effects of salvianolic acid B (Sal B) on acute myocardial ischemia reperfusion (MIR) injury rats, on the basis of this investigation, the possible mechanism of salvianolic acid B was elucidated. Male Sprague-Dawley rats (200-220 g) were randomly divided into five groups: sham-operated, MIR, MIR + Sal B (10 mg/kg/day, orally), MIR + Sal B (20 mg/kg/day, orally) and MIR + Sal B (30 mg/kg/day, orally). Before operation, the foregoing groups were pretreated with homologous drug once a day for 7 days, respectively. After twelve hours in MIR, the cardioprotective effects of SPJ were evaluated by infarct size, biochemical values, and the antioxidative and antiapoptotic relative gene expressions. Results: Sal B significantly improved heart function and decreased infarct size; remarkably decreased levels of serum TNF-α and IL-1β levels, increased contents of myocardium antioxidant enzymes activities; western blot results showed that Sal B ameliorate the increased Bax and caspase-3 protins expressions and decreased Bcl-2 proteins expression and ratios of Bcl-2 to Bax. Conclusion: In ischemic myocardium, oxidative stress caused the overgeneration and accumulation of reactive oxygen species (ROS), which was central of cardiac ischemic injury. Sal B exerted beneficially cardioprotective effects on myocardial ischemia injury rats, mainly scavenging oxidative stress-triggered overgeneration and accumulation of ROS, alleviating myocardial ischemia injury and cardiac cell death.

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Section: Discussionsupporting

confidence: 94%

Myocardium Protective Function of Salvianolic Acid B in Ischemia Reperfusion Rats

Qiao

2016

Afr J Tradit Complement Altern Med

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“…However, spleen is one of the most susceptible immune organs to fluoride because of the accumulation of fluoride ions in its cells (Krishnamoorthy et al, 2015). Studies indicated that excessive fluoride ingestion induced mitochondrial damage and cellular apoptosis through larger accumulation of fluoride ions in cells (Panneerselvam et al, 2015; Sarkar et al, 2014, Wang et al, 2017b). In the present study, the structure of pathomorphological and subcellular organelles in the spleen was seriously damaged after fluoride treatment.…”

Section: Discussionmentioning

confidence: 99%

Induction of pathological changes and impaired expression of cytokines in developing female rat spleen after chronic excess fluoride exposure

et al. 2018

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This study was designed to investigate the effects of excessive fluoride on spleen toxicity. Twenty-four healthy female rats were randomly divided into two groups, each of 12 rats. Each group of female rats was given a control diet and either F− = 0 mg/L or an excessive F− = 150 mg/L in the drinking water for 120 days. The histomorphological and ultrastructural changes in their splenic tissues were observed under light and transmission electron microscopes. DNA damage and splenocyte apoptosis were examined using the micronucleus (MN) assay, single-cell gel electrophoresis (SCGE), and flow cytometry. The expression levels of cytokines, including interleukin (IL)-1β, IL-2, IL-6, and tumor necrosis factor (TNF)-α, were determined through immunohistochemistry and Western-blot analysis. Results demonstrated that the histomorphological characteristics and ultrastructure of the splenic tissues were affected by excessive fluoride. Nuclear dying, nuclear membrane dissolution, mitochondrial vacuolation, and endoplasmic reticulum dilation were observed. SCGE and MN assays showed that the nuclear DNA of splenocytes was damaged by fluoride treatment, and splenocyte apoptosis was exacerbated in the fluoride group. With damage to the splenocyte structure and DNA, the protein expression levels of IL-1β, IL-2, IL-6, and TNF-α were significantly downregulated by exposure to fluoride. Excessive fluoride ingestion caused splenic pathological damage and abnormal cytokine expression in female rats.

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“…Fluoride can enter the cells; in excessive amounts it can cause serious damage to the cytoskeleton, nuclear condensation, myocardial fiber breakage, calcium overload, and mitochondrial dissolution, which impairs ATP production [65,66]. The mentioned changes could explain myocardial ischemia, myocardial infarction, and heart failure associated with high fluoride intake, involving increased oxidative stress, apoptosis, and necrosis [67]. Fluoride in drinking water is nephrotoxic according to a study performed in rats with experimental chronic kidney disease, increasing the incipient aortic calcifications [68].…”

Section: Fluoridesmentioning

confidence: 99%

Oral Health and Cardiovascular Disorders

Mozoş¹,

Stoian²

2020

Understanding the Molecular Crosstalk in Biological Processes

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Several studies reported the cross talk between oral health and cardiovascular disorders. The aim of the present chapter is to review the main mechanisms linking oral and cardiovascular disorders, the main pathologies which could be linked, and possibilities for prophylactic and therapeutic interventions. Periodontitis was associated with cardiovascular risk, and the links between the two entities are represented by bacteria and their toxins released into the blood, causing endothelial dysfunction and providing a proatherogenic and prothrombotic effect and an inflammatory and immune reaction. The mentioned mechanisms explain the reported associations of periodontitis with stroke, coronary heart disease, and peripheral vascular disease. Periodontitis was also associated with diabetes mellitus and impaired lipid metabolism. Not all studies confirmed the association between periodontitis and coronary artery disease or stroke. Tooth loss, the most important consequence of periodontitis, has been also associated with cardiovascular disease. Dental and pulpal caries were also found to be independent risk factors for atherosclerosis, while restorations were inversely related to an atherosclerotic burden. Sucrose is involved in both cariogenesis and atherosclerosis. Fluorides prevent aortic calcifications and enamel demineralization and inhibit bacterial metabolism but are cardiotoxic. Heightening awareness of good dental hygiene can improve cardiovascular health.

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Single oral acute fluoride exposure causes changes in cardiac expression of oxidant and antioxidant enzymes, apoptotic and necrotic markers in male rats

Cited by 26 publications

References 46 publications

Myocardium Protective Function of Salvianolic Acid B in Ischemia Reperfusion Rats

Myocardium Protective Function of Salvianolic Acid B in Ischemia Reperfusion Rats

Induction of pathological changes and impaired expression of cytokines in developing female rat spleen after chronic excess fluoride exposure

Oral Health and Cardiovascular Disorders

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