Effects of single and combined use of bacteriophages and antibiotics to inactivate Escherichia coli

Valério, Nádia; Oliveira, Cristiana; Jesus, Vânia Isabel Rodrigues; Branco, Tatiana Moura; Pereira, Carla; Moreirinha, Catarina; Almeida, Adelaide

doi:10.1016/j.virusres.2017.07.015

Cited by 86 publications

(76 citation statements)

References 36 publications

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“…Additionally, to minimize the potential for the development of resistance, a combination of phages with antibiotics might be required, since there is usually no cross-resistance with antibiotics [52]. Besides, when combined with antibiotics, phages are likely to enhance the absorption of antibiotics into bacterial cells [53][54][55][56]. Further study is required to evaluate the effectiveness of the combining phages with antibiotics in the treatment of A.…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of bacteriophages against virulent Aeromonas hydrophila

Jin

Gao

Dong

et al. 2020

Preprint

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Background: Aeromonas hydrophila is an important water-borne pathogen that leads to a great economic loss in aquaculture. Along with the abuse of antibiotics, drug-resistant strains rise rapidly. In addition, the biofilms formed by this bacterium limited the antibacterial effect of antibiotics. Bacteriophages have been attracting increasing attention as a potential alternative to antibiotics against bacterial infections. Results: Five phages against pathogenic A. hydrophila, named N21, W3, G65, Y71 and Y81, were isolated. Morphological analysis by transmission electron microscopy revealed that phages N21, W3 and G65 belong to the family Myoviridae, while Y71 and Y81 belong to the Podoviridae. These phages were found to have broad host spectra, short latent periods and normal burst sizes. They were sensitive to high temperature but had a wide adaptability to the pH. In addition, the phages G65 and Y81 showed considerable bacterial killing effect and potential in preventing formation of A. hydrophila biofilm; and the phages G65, W3 and N21 were able to scavenge mature biofilm effectively. Phage treatments applied to the pathogenic A. hydrophila in mice model resulted in a significantly decreased bacterial loads in tissues. Conclusions: Five A. hydrophila phages were isolated with broad host ranges, low latent periods, and wide pH and thermal tolerance. And the phages exhibited varying abilities in controlling A. hydrophila infection. This work presents promising data supporting the future use of phage therapy.

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Section: Discussionmentioning

confidence: 99%

Isolation and characterization of bacteriophages against virulent Aeromonas hydrophila

Jin

Gao

Dong

et al. 2020

Preprint

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“…Valério et al [85] observed an absence of either phage-mediated killing of E. coli or phage ECA2 (10 7 PFU/mL) population growth while in the presence of chloramphenicol or tetracycline at 1× MIC. They also observed an absence of phage population growth and probably a lack of phage-mediated bacteria killing in the presence of 1× MIC ciprofloxacin.…”

Section: Valério Et Al 2017 Escherichia Coli Various Antibioticsmentioning

confidence: 99%

Phage-Antibiotic Combination Treatments: Antagonistic Impacts of Antibiotics on the Pharmacodynamics of Phage Therapy?

Abedon

2019

Antibiotics

101

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Bacteria can evolve resistance to antibiotics. Even without changing genetically, bacteria also can display tolerance to antibiotic treatments. Many antibiotics are also broadly acting, as can result in excessive modifications of body microbiomes. Particularly for antibiotics of last resort or in treating extremely ill patients, antibiotics furthermore can display excessive toxicities. Antibiotics nevertheless remain the standard of care for bacterial infections, and rightly so given their long track records of both antibacterial efficacy and infrequency of severe side effects. Antibiotics do not successfully cure all treated bacterial infections, however, thereby providing a utility to alternative antibacterial approaches. One such approach is the use of bacteriophages, the viruses of bacteria. This nearly 100-year-old bactericidal, anti-infection technology can be effective against antibiotic-resistant or -tolerant bacteria, including bacterial biofilms and persister cells. Ideally phages could be used in combination with standard antibiotics while retaining their anti-bacterial pharmacodynamic activity, this despite antibiotics interfering with aspects of bacterial metabolism that are also required for full phage infection activity. Here I examine the literature of pre-clinical phage-antibiotic combination treatments, with emphasis on antibiotic-susceptible bacterial targets. I review evidence of antibiotic interference with phage infection activity along with its converse: phage antibacterial functioning despite antibiotic presence.

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“…In natural environments, antibiotic and phage resistance phenotypes are thought to evolve independently . Therefore, the combined use of antibiotics and phages should result in better bacterial clearance and prevent resistant phenotypes from developing . Moreover, bacterial resistance to phages can result from bacterial adaptation to evade phage infection or from a lack of phage specificity to the bacterium .…”

Section: Phage Therapymentioning

confidence: 99%

“…55 Therefore, the combined use of antibiotics and phages should result in better bacterial clearance and prevent resistant phenotypes from developing. 56 Moreover, bacterial resistance to phages can result from bacterial adaptation to evade phage infection or from a lack of phage specificity to the bacterium. 57 In clinical settings, correct phage selection and the analysis of susceptibility of the target bacteria to the phage therapeutic product, or phagograms, should be complementary and mandatory.…”

Section: Phag E Ther Apymentioning

confidence: 99%

Review article: bacteriophages in gastroenterology—from biology to clinical applications

Sabino

Hirten

Colombel

2019

Aliment Pharmacol Ther

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Summary Background The gut microbiota plays an important role in the pathogenesis of several gastrointestinal diseases. Its composition and function are shaped by host‐microbiota and intra‐microbiota interactions. Bacteriophages (phages) are viruses that target bacteria and have the potential to modulate bacterial communities. Aims To summarise phage biology and the clinical applications of phages in gastroenterology Methods PubMed was searched to identify relevant studies. Results Phages induce bacterial cell lysis, integration of viral DNA into the bacteria and/or coexistence in a stable equilibrium. Bacteria and phages have co‐evolved and their dynamic interactions are yet to be fully understood. The increasing need to modulate microbial communities (e.g., gut microbiota, multidrug‐resistant bacteria) has been a strong stimulus for research in phages as an antibacterial therapy. In gastroenterology, phage therapy has been mainly studied in infectious diseases such as cholera. However, it is currently being explored in several other circumstances such as treating Clostridioides difficile colitis, targeting adherent‐invasive Escherichia coli in Crohn's disease or eradicating Fusobacterium nucleatum in colorectal cancer. Overall, phage therapy has a favourable and acceptable safety profile. Presently, trials with phage therapy are ongoing in Crohn's disease. Conclusions Phage therapy is a promising therapeutic tool against pathogenic bacteria in the fields of infectious diseases and gastroenterology. Randomised, placebo‐controlled trials with phage therapy for gastroenterological diseases are ongoing.

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Effects of single and combined use of bacteriophages and antibiotics to inactivate Escherichia coli

Cited by 86 publications

References 36 publications

Isolation and characterization of bacteriophages against virulent Aeromonas hydrophila

Isolation and characterization of bacteriophages against virulent Aeromonas hydrophila

Phage-Antibiotic Combination Treatments: Antagonistic Impacts of Antibiotics on the Pharmacodynamics of Phage Therapy?

Review article: bacteriophages in gastroenterology—from biology to clinical applications

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