Effects of silver nanoparticles on primary cell cultures of fibroblasts and keratinocytes in a wound-healing model

Franková, Jana; Pivodová, Veronika; Vágnerová, Hana; Juráňová, Jana; Ulrichová, Jitka

doi:10.5301/jabfm.5000268

Cited by 93 publications

(98 citation statements)

References 37 publications

(44 reference statements)

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“…A number of in vitro studies have been performed exploring the effects of AgNPs on a variety of cell types [88,239,244,245,246,247,248,249,250,251,252,253]. The most common mechanisms of toxicity from nanosized silver particles, as well as silver ions released from them [245,254] are: oxidative stress [246,255], Trojan-horse mechanisms [256,257] and DNA damage [163].…”

Section: Potential Side Effects Of Silver Nanoparticlesmentioning

confidence: 99%

Silver Nanocoating Technology in the Prevention of Prosthetic Joint Infection

et al. 2016

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Prosthetic joint infection (PJI) is a feared complication of total joint arthroplasty associated with increased morbidity and mortality. There is a growing body of evidence that bacterial colonization and biofilm formation are critical pathogenic events in PJI. Thus, the choice of biomaterials for implanted prostheses and their surface modifications may significantly influence the development of PJI. Currently, silver nanoparticle (AgNP) technology is receiving much interest in the field of orthopaedics for its antimicrobial properties and a strong anti-biofilm potential. The great advantage of AgNP surface modification is a minimal release of active substances into the surrounding tissue and a long period of effectiveness. As a result, a controlled release of AgNPs could ensure antibacterial protection throughout the life of the implant. Moreover, the antibacterial effect of AgNPs may be strengthened in combination with conventional antibiotics and other antimicrobial agents. Here, our main attention is devoted to general guidelines for the design of antibacterial biomaterials protected by AgNPs, its benefits, side effects and future perspectives in PJI prevention.

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Section: Potential Side Effects Of Silver Nanoparticlesmentioning

confidence: 99%

Silver Nanocoating Technology in the Prevention of Prosthetic Joint Infection

et al. 2016

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“…Rapid wound healing with better scar appearance and normal hair growth in the AgNPs-treated group, as compared to silver sulfadiazinetreated group, was observed. Franková et al 72 have studied the in vitro effect of AgNPs as wound-healing promoters. They have reported that AgNPs decreased the release of some pro-inflammatory cytokines and growth factors from normal human epidermal keratinocytes (NHEKs) and normal human dermal fibroblasts after 24 h at all the studied AgNPs concentrations (0.25, 2.5, and 25 µg/mL).…”

Section: Wound Healingmentioning

confidence: 99%

In vitro and in vivo evaluation of biologically synthesized silver nanoparticles for topical applications: effect of surface coating and loading into hydrogels

Mekkawy

El‐Mokhtar²,

Nafady

et al. 2017

IJN

150

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In the present study, silver nanoparticles (AgNPs) were synthesized via biological reduction of silver nitrate using extract of the fungus Fusarium verticillioides (green chemistry principle). The synthesized nanoparticles were spherical and homogenous in size. AgNPs were coated with polyethylene glycol (PEG) 6000, sodium dodecyl sulfate (SDS), and β-cyclodextrin (β-CD). The averaged diameters of AgNPs were 19.2±3.6, 13±4, 14±4.4, and 15.7±4.8 nm, for PEG-, SDS-, and β-CD-coated and uncoated AgNPs, respectively. PEG-coated AgNPs showed greater stability as indicated by a decreased sedimentation rate of particles in their water dispersions. The antibacterial activities of different AgNPs dispersions were investigated against Gram-positive bacteria (methicillin-sensitive and methicillin-resistant Staphylococcus aureus ) and Gram-negative bacteria ( Escherichia coli ) by determination of the minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs). MIC and MBC values were in the range of 0.93–7.5 and 3.75–15 µg/mL, respectively, which were superior to the reported values in literature. AgNPs-loaded hydrogels were prepared from the coated-AgNPs dispersions using several gelling agents (sodium carboxymethyl cellulose [Na CMC], sodium alginate, hydroxypropylmethyl cellulose, Pluronic F-127, and chitosan). The prepared formulations were evaluated for their viscosity, spreadability, in vitro drug release, and antibacterial activity, and the combined effect of the type of surface coating and the polymers utilized to form the gel was studied. The in vivo wound-healing activity and antibacterial efficacy of Na CMC hydrogel loaded with PEG-coated AgNPs in comparison to the commercially available silver sulfadiazine cream (Dermazin ® ) were evaluated. Superior antibacterial activity and wound-healing capability, with normal skin appearance and hair growth, were demonstrated for the hydrogel formulations, as compared to the silver sulfadiazine cream. Histological examination of the treated skin was performed using light microscopy, whereas the location of AgNPs in the skin epidermal layers was visualized using transmission electron microscopy.

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“…Cytokines such as interleukin-6 (IL-6), IL-1β, tumour necrosis factor-α (TNF-α), interferon-γ (IFN-γ), and transforming growth factorβ (TGF-β) are produced by the leucocyte cells and stimulate changes in the production of acute phase plasma proteins as well as many other biochemical and physiological changes. In acute inflammation, the leucocyte cells are mainly neutrophils, while in chronic inflammation macrophages and lymphocyte cells are recruited approximately 48-96 hrs after initiation to aid in destroying the inflammatory agent and promote healing [89,91]. The nuclear factor kappa B (NF-B) pathway is involved in the cellular response to various stresses including oxidative stress, and is involved in the start of inflammation.…”

Section: Nanosilver and Inflammationmentioning

confidence: 99%

A Current Overview of the Molecular Effects of Nanosilver

Cameron¹,

Willmore²

2018

Preprint

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Nanosilver plays an important role in nanoscience and nanotechnology, and is becoming increasingly used for applications in nanomedicine. Nanosilver ranges in size from one to one hundred nanometers. Smaller particles more readily enter cells and interact with the cellular components. The exposure dose, particle size, coating, and aggregation state of the nanosilver, as well as the cell type or organism that it is tested on, all have a large determining factor on the effect and potential toxicity of nanosilver. A high exposure dose to nanosilver alters the cellular stress responses and initiates cascades of signaling that can eventually trigger organelle autophagy and apoptosis. This review summarizes the current knowledge of the effects of nanosilver on cellular metabolic function and response to stress. Both the causative effects of nanosilver on oxidative stress, endoplasmic reticulum stress, and hypoxic stress, as well as the effects of nanosilver on the responses to such stresses, are outlined. The interactions and effects of nanosilver on cellular uptake, oxidative stress (reactive oxygen species), inflammation, hypoxic response, mitochondrial function, endoplasmic reticulum function and the unfolded protein response, autophagy and apoptosis, angiogenesis, epigenetics, genotoxicity, and cancer development and tumorigenesis, as well as other pathway alterations are examined in this review.

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Effects of silver nanoparticles on primary cell cultures of fibroblasts and keratinocytes in a wound-healing model

Abstract: The results indicate that NHEKs are more susceptible to the application of AgNPs than NHDFs, and AgNPs may be useful for medical applications for the treatment of wounds.

Cited by 93 publications

References 37 publications

Silver Nanocoating Technology in the Prevention of Prosthetic Joint Infection

Silver Nanocoating Technology in the Prevention of Prosthetic Joint Infection

In vitro and in vivo evaluation of biologically synthesized silver nanoparticles for topical applications: effect of surface coating and loading into hydrogels

A Current Overview of the Molecular Effects of Nanosilver

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