Effects of silencing S100A8 and S100A9 with small interfering RNA on the migration of CNE1 nasopharyngeal carcinoma cells

Abstract

Abstract. The calcium-binding S100 proteins are involved in functions such as cell growth, differentiation, migration, adhesion and signal transduction. S100A8 and S100A9 are highly expressed in a variety of tumor cells, and are implicated in tumor development and progression. However, the role of S100A8 and S100A9 in nasopharyngeal carcinoma (NPC) cell migration is unclear. The present study investigated the effect of S100A8 and S100A9 on migration using a NPC cell line, CNE1. The CNE1 cells were transfected with S100A8 or S100A9 small interfering RNA (siRNA). Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to detect S100A8 and S100A9 gene expression. Following the downregulation of S100A8 or S100A9, the effects on cell migration were determined using wound-healing assays. The expression of matrix metalloproteinase-7 (MMP7), a member of the MMP family that is associated with tumor cell invasion and migration, was also detected by RT-qPCR. S100A8 and S100A9 siRNAs effectively suppressed S100A8 and S100A9 gene expression, and substantially inhibited the migration of the CNE1 cells. In addition, MMP7 expression was reduced to varying extents in S100A8 and S100A9 siRNA-treated cells compared with controls. Thus, S100A8 and S100A9 promoted the migration of CNE1 NPC cells.

The role of <em>calgranulin B</em> gene on the biological behavior of squamous cervical cancer in vitro and in vivo

“… 31 Calgranulin B was demonstrated to increase the expression of MMP7, enhancing the migration of CNE1 nasopharyngeal carcinoma cells. 32 However, in the present study, the transcription of calgranulin B gene was found to be negatively correlated with MMP15 and MMP24 genes in cervical cancer. Furthermore, a positive relation was found between calgranulin B gene and MMP25 genes, indicating that calgranulin B-induced migration and invasion of cervical cancer cells may be involved in the regulation of MMPs , except MMP2 .…”

Meta-analysis of gene expression in relapsed childhood B-acute lymphoblastic leukemia

“…Moreover, increased expression of S100A8 mediated the activation of MAPK and NF-κB pathways, and treatment with p38 MAPK inhibitor SB203580 and the NF-κB inhibitor Bay 11-7082 effectively abolished migration and invasion of cancer cells [ 39 ]. Other than conferring selective sensitivity to drugs which target mediators of S100A8, the knockdown of S100A8 expression with siRNA or shRNA also showed reduced invasinesss and migration of cancer cells [ 28 , 34 , 36 , 37 ]. Taken together, S100A8 is an ideal target for relapsed ALL therapy, and warrants further investigation.…”

S100A8/A9 Acts as Prognostic Indicators and Promotes Migration and Invasion via p38 MAPK Pathway in&nbsp;Nasopharyngeal Carcinoma

“…We previously found S100A8/A9 proteins were overexpressed in NPC cells and silencing of endogenous S100A8/A9 could signi cantly reduce NPC cell migration ability. [30] S100A8/A9 as a pair of secreted soluble in ammatory factors was also detected in the intercellular space besides the tumor cell cytoplasm in these NPC tissues as indicated above. To explore the effects of exogenous S100A8/A9 on NPC cell proliferation, we treated the NPC cells with S100A8/A9 at different concentrations to mimic S100A8/A9 in ltrated NPC microenvironment and detected cell proliferation by CCK-8 assay.…”

“…We previously discovered that S100A8 and S100A9 knockdown could signi cantly reduce the expressions of matrix metalloproteinases (MMPs) in NPC cancer cells [30]. In the present study, we further explored the downstream of signaling pathway and evaluated the β-catenin and MMP7 expression levels in clinical NPC tissues and chronic pharyngitis (CP) tissues, which are two important proteins in tumor cell invasion and migration.…”