Effects of Short-Term Exercise on Adiponectin and Adiponectin Receptor Levels in Rats

Abstract

Aim: Adiponectin reportedly reduces insulin resistance. Exercise has also been shown to lessen insulin resistance, although it is not well known whether exercise increases levels of adiponectin and/or its receptors nor whether it effects are dependent on exercise intensity and/or period. We previously reported that blood adiponectin levels increased by 150% in animals that exercised at a rate of 30 m/min for 60 minutes, 2 days per week, and adiponectin receptor 1 (AdipoR1) mRNA levels in muscle increased up to 4 times in response to exercise at a rate of 25 m/min for 30 min, 5 days per week for 12 weeks. Methods: In light of this information, we examined the effects of short-term exercise on adiponectin, and adiponectin receptor levels in rats, using ELISA and real-time PCR. Results: Our data showed that adiponectin mRNA levels in adipose tissue increased by 280% in rats exercised at a rate of 30 m/min for 60 minutes for 2 weeks and correlated with the exercise time periods. No effects of short-term exercise on adiponectin receptor 1 mRNA in muscle were observed. Conclusion: Thus, long-term exercise may be required to regulate adiponectin receptor 1 mRNA expression in muscle and adiponectin mRNA expression in adipose tissue.

Differential Effects of Acute (Extenuating) and Chronic (Training) Exercise on Inflammation and Oxidative Stress Status in an Animal Model of Type 2 Diabetes Mellitus

“…There is interplay between the oxidation of lipids and carbohydrates during exercise, with lower intensities associated with a greater percentage of FFAs oxidation and higher intensities with greater carbohydrate utilization; exercise intensity also affects the reliance on the form (glycogen or glucose) of carbohydrate oxidized [51]. In studies using rodent, adiponectin has been shown to regulate plasma FFAs clearance by stimulating skeletal muscle FFA uptake [52] and/or oxidation [53]. Our results showed in the diabetic ZDF rats that underwent acute strenuous exercise an augment of adiponectin levels (+5.8%; P < 0.05).…”

Voluntary Exercise Ameliorates the Progression of Atherosclerotic Lesion Formation via Anti-Inflammatory Effects in Apolipoprotein E-Deficient Mice

“…A recent clinical interventional study demonstrated that the increase in adiponectin concentration in response to physical exercise was related to a reduction in fat mass and inflammatory factor levels 24) . Adiponectin mRNA levels in epididymal fat tissue increased in rats after treadmill exercise 25) . Furthermore, 3T3-L1 adipocytes treated with TNF-showed decreased adiponectin mRNA expression 26) , suggesting that TNF- Serum IL-6 and TNF-levels were measured using the Cytometric Bead Array Mouse Soluble Protein Master Buffer Kit in the STD (n 8), HFD (n 6), and HFD Ex (n 6) groups at 18 weeks of age.…”

HMW-Adiponectin Associates with Triglyceride Concentrations in Type 1 Diabetic Patients

“…The decreased effects of adiponectin on glucose uptake coupled with the acceleration of fatty acid oxidation, observed in current study, suggest an increase in very low density lipoprotein receptor expression and/or a decrease in the AdipoR1/R2 ratio in patients with a high HMWadiponectin concentration. In addition, adiponectin receptor mRNA expression was reported to correlate with diet and exercise 39,40) . Differences in the expression of these receptors need to be studied to clarify the influences of HMW-adiponectin on insulin therapy in patients with type 1 diabetes.…”