Effects of shading on triterpene saponin accumulation and related gene expression of Aralia elata (Miq.) Seem

Cheng, Yao; Liu, Hanbing; Tong, Xuejiao; Liu, Zaimin; Zhang, Xin; Chen, Yingtong; Wu, Fengzhi; Jiang, Xinhong; Yu, Xiaoyan

doi:10.1016/j.plaphy.2021.01.009

Cited by 13 publications

(15 citation statements)

References 32 publications

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“…Seem, in contrast to squalene synthase (SQS) and squalene epoxidase (SQE), two other enzymes of the isoprenoid metabolic pathway. However, more excessive shading downregulated the expression of all three enzymes [ 223 ]. The effect of light on the isoprenoid pathway may be connected to the redox system since the SQE-deficient dry2/sqe1-5 Arabidopsis mutant showed deficient ROS production and NADPH oxidase function [ 224 ].…”

Section: Light Intensity- and Spectrum-associated Adjustment Of Metab...mentioning

confidence: 99%

Light Intensity- and Spectrum-Dependent Redox Regulation of Plant Metabolism

et al. 2022

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Both light intensity and spectrum (280–800 nm) affect photosynthesis and, consequently, the formation of reactive oxygen species (ROS) during photosynthetic electron transport. ROS, together with antioxidants, determine the redox environment in tissues and cells, which in turn has a major role in the adjustment of metabolism to changes in environmental conditions. This process is very important since there are great spatial (latitude, altitude) and temporal (daily, seasonal) changes in light conditions which are accompanied by fluctuations in temperature, water supply, and biotic stresses. The blue and red spectral regimens are decisive in the regulation of metabolism because of the absorption maximums of chlorophylls and the sensitivity of photoreceptors. Based on recent publications, photoreceptor-controlled transcription factors such as ELONGATED HYPOCOTYL5 (HY5) and changes in the cellular redox environment may have a major role in the coordinated fine-tuning of metabolic processes during changes in light conditions. This review gives an overview of the current knowledge of the light-associated redox control of basic metabolic pathways (carbon, nitrogen, amino acid, sulphur, lipid, and nucleic acid metabolism), secondary metabolism (terpenoids, flavonoids, and alkaloids), and related molecular mechanisms. Light condition-related reprogramming of metabolism is the basis for proper growth and development of plants; therefore, its better understanding can contribute to more efficient crop production in the future.

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Section: Light Intensity- and Spectrum-associated Adjustment Of Metab...mentioning

confidence: 99%

Light Intensity- and Spectrum-Dependent Redox Regulation of Plant Metabolism

et al. 2022

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“…Those medicinal potentials were depended on the bioactive components of saponins. More than 100 kinds of saponins belonging to triterpene saponins have been reported in A. elata and they are mainly oleananetype saponins (Cheng et al, 2021) The triterpenoid biosynthesis is initiated from isopentenyl diphosphate (IPP) that is derived from the metabolism of cytosolic mevalonic acid (MVA) or the plastid methylerythritol phosphate (MEP; Sawai and Saito, 2011). This biosynthesis process is catalyzed by a series of key enzymes.…”

Section: Introductionmentioning

confidence: 99%

“…(Araliaceae), also known as Chinese angelica-tree, is widely distributed in Northeast China (mainly Heilongjiang and Jilin province), Korea, Japan, Russia, the south of Far East, the south of Sakhalin, and Kuril Islands ( Ahn, 1998 ; Reunov et al, 2007 ). It is one of the most desirable mountain wild vegetables in Asia benefitting from the rich nutrients in its young shoots ( Cheng et al, 2021 ). Furthermore, as a Chinese traditional medicinal plant, A. elata plays roles on rheumatism, diabetes, hepatitis, neurasthenia, and stomach spasms ( Zhang et al, 2018 ), especially the anti-tumor role ( Duan et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

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A High-Quality Reference Genome Sequence and Genetic Transformation System of Aralia elata

et al. 2022

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Aralia elata is a perennial woody plant of the genus Aralia in the family Araliaceae. It is rich in saponins and therefore has a wide range of pharmacological effects. Here, we report a high-quality reference genome of A. elata, with a genome size of 1.21 Gb and a contig N50 of 51.34 Mb, produced by PacBio HiFi sequencing technology. This is the first genome assembly for the genus Aralia. Through genome evolutionary analysis, we explored the phylogeny and whole genome duplication (WGD) events in the A. elata genome. The results indicated that a recent WGD event occurred in the A. elata genome. Estimation of the divergence times indicated that the WGD may be shared by Araliaceae. By analyzing the genome sequence of A. elata and combining the transcriptome data from three tissues, we discovered important genes related to triterpene saponins biosynthesis. Furthermore, based on the embryonic callus induction system of A. elata established in our laboratory, we set up the genetic transformation system of this plant. The genomic resources and genetic transformation system obtained in this study provide insights into A. elata and lays the foundation for further exploration of the A. elata regulatory mechanism.

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“…A recent work revealed that the increased total terpene contents in drought-stressed seedlings of Douglas firs could be a result of altered carbon allocation under conditions of water shortage, resulting in decreased seedling growth and the allocation of larger fractions of assimilated carbon to terpenoid biosynthesis [ 15 ]. In a 40% shade condition with a sunshade net, the total triterpene saponin in leaves was observed to have the greatest yield compared to that in a sunlight condition [ 27 ].…”

Section: Introductionmentioning

confidence: 99%

Acclimation Strategy of Masson Pine (Pinus massoniana) by Limiting Flavonoid and Terpenoid Production under Low Light and Drought

Shi

Deng

Zeng

et al. 2022

IJMS

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Low light and drought often limit the growth and performance of Masson pines (Pinus massoniana) in the subtropical forest ecosystem of China. We speculated that stress-induced defensive secondary metabolites, such as flavonoids and terpenoids, might influence the growth of Masson pines, considering the existence of tradeoffs between growth and defense. However, the mechanisms of Masson pines responsive to low light and drought at the levels of these two metabolites remain unclear. In the present work, the compositions of flavonoids and terpenoids, as well as their biosynthetic pathways, were revealed through metabolome and transcriptome analyses, respectively, coupled with a study on carbon allocation using a 13CO2-pulse-labeling experiment in two-year-old seedlings under low light (LL), drought (DR), and their combined stress (DL) compared to a control (CK). A total of 35 flavonoids and derivatives (LL vs. CK: 18; DR vs. CK: 20; and DL vs. CK: 18), as well as 29 terpenoids and derivatives (LL vs. CK: 23; DR vs. CK: 13; and DL vs. CK: 7), were differentially identified in the leaves. Surprisingly, most of them were decreased under all three stress regimes. At the transcriptomic level, most or all of the detected DEGs (differentially expressed genes) involved in the biosynthetic pathways of flavonoids and terpenoids were downregulated in phloem and xylem under stress treatments. This indicated that stress treatments limited the production of flavonoids and terpenoids. The reduction in the 13C allocation to stems might suggest that it is necessary for maintaining the growth of Masson pine seedlings at the whole-plant level by attenuating energetic resources to the biosynthetic pathways of flavonoids and terpenoids when facing the occurrence of adverse environments. Our results provide new insight into understanding the acclimation strategy of Masson pines or other conifers in adverse environments.

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Effects of shading on triterpene saponin accumulation and related gene expression of Aralia elata (Miq.) Seem

Cited by 13 publications

References 32 publications

Light Intensity- and Spectrum-Dependent Redox Regulation of Plant Metabolism

Light Intensity- and Spectrum-Dependent Redox Regulation of Plant Metabolism

A High-Quality Reference Genome Sequence and Genetic Transformation System of Aralia elata

Acclimation Strategy of Masson Pine (Pinus massoniana) by Limiting Flavonoid and Terpenoid Production under Low Light and Drought

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