Effects of sensorial denervation induced by capsaicin injection at birth or on day three of life, on puberty, induced ovulation and pregnancy

Morán, Carolina; Morales, Leticia; Razo, Rocı́o Selene; Apolonio, Javier; Quiróz, Ubaldo; Chavira, Roberto; Domı́nguez, Roberto

doi:10.1016/s0024-3205(03)00598-8

Cited by 27 publications

(17 citation statements)

References 36 publications

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“…The results indicate that in adult rats the establishment of compensatory ovarian hypertrophy depended on the side of ovariectomy (the side of the remaining ovary) and on the day of the estrous cycle when the pharmacological denervation was performed (Trujillo et al, 2004). In addition, in rats treated with capsaicin at birth and hemiovariectomized at 28 days of age compensatory ovulation by the right ovary was higher than in controls, while compensatory ovulation from the left ovary decreased significantly (Moran et al, 2003). These observations strongly suggest that the neuralborn information from the left and right ovary conveyed to the central nervous system is different.…”

Section: Discussionmentioning

confidence: 84%

Predominance of supraspinal innervation of the left ovary

Tóth

Wiesel

Boldogköi

et al. 2007

Microscopy Res & Technique

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In our previous studies using the viral transneuronal tracing technique we demonstrated the spinal and supraspinal components of the ovarian innervation. Since increasing number of data indicate the presence of morphological and functional laterality in the control of gonadal functions, we aimed to investigate whether cerebral structures trans-synaptically involved in the innervation of the ovary exhibit asymmetry or not. In one of the studies the left or the right ovary was injected with the red fluorescent protein expressing pseudorabies virus and the number of infected "red" autofluorescent neurons from the right and the left ovary was compared. In another study in order to have distinct labeling of cell groups connected with the right- and left-sided ovary in the same animal, a dual viral labeling was applied. The left- and right-sided ovary were inoculated with genetically engineered pseudorabies virus expressing a red fluorescent protein or a green fluorescent protein gene. Viral infection of brain nuclei including the dorsal vagal nucleus, caudal raphe nuclei, A5 noradrenergic cell group, hypothalamic paraventricular nucleus, from the left ovary in each case was enhanced when compared with labeling from the right gonad. Data suggest a predominance in the supraspinal innervation of the left ovary.

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Section: Discussionmentioning

confidence: 84%

Predominance of supraspinal innervation of the left ovary

Tóth

Wiesel

Boldogköi

et al. 2007

Microscopy Res & Technique

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“…Numerous studies support the notion of the importance of innervation on the regulation of the gonadal functions [1][2][3][4][5]. Recent studies by Gerendai et al [6], using a viral tracing technique, have provided the first morphological evidence of the existence of a neuronal multi-synaptic way between the ovary and various centers of the central nervous system, through the sympathetic ganglionic pathway.…”

Section: Introductionmentioning

confidence: 97%

Release of ovarian progesterone during the rat oestrous cycle by ganglionic cholinergic influence

Sosa

Delgado

Casais

et al. 2004

The Journal of Steroid Biochemistry and Molecular Biology

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“…Then, a blood sample was obtained from the tail veins of the animals following an 8-h fasting period 72 h after STZ injection and rats having a blood glucose level of 200 mg/dl or higher measured by a hand glucometer (Accu-Chek-Go, Roche, Switzerland) were considered diabetic [27] and included in the study. DEPREM, ÇINAR After the third day, when diabetes was confirmed, Group II and Group III were subcutaneously injected with capsaicin 1 mg/kg (Sigma, St Louis, MO, USA) (dissolved in 10% ethanol, 1% Tween 20, and 80% distilled water) every day for 2 weeks [28] .…”

Section: Experimental Animalsmentioning

confidence: 99%

Kapsaisin Uygulanan Diabetik Sıçanların Karaciğer Dokusunda GPX 1’in Real Time-PCR ile Expresyon Profilinin Çıkarılması

Kürüm¹,

Kocamiş²,

Deprem³

et al. 2015

Kafkas Univ Vet Fak Derg

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The purpose of the present study was to determine the Glutathione Peroxidase 1 (GPX 1) gene expression by Real Time PCR in the liver of healthy and diabetic rats treated with capsaicin. Twenty Sprague-Dawley rats were used in our study. Rats were divided into four groups: Group I: diabetic rats (n=5), Group II: capsaicin injected rats (n=5), Group III: Capsaicin injected diabetic rats (n=5), and Group IV: control rats (n=5). Capsaicin injection began 72 h after streptozotocin (STZ) injection. Capsaicin (1 mg/kg) was prepared in 10% ethanol, 1% Tween 20, and 80% sterile water and subcutaneously injected daily in both Groups II and III for two weeks. The results of RT-PCR conducted to determine GPX 1 gene expression indicated that capsaicin treated diabetic rats had higher GPX 1 expression compared to all groups including control (P<0.05). As a result, capsaicin causes an increase in GPX 1 gene expression at transcription level in the diabetic rat liver. Further investigation is needed to determine if such an increase occurs at protein level using various methods such as western-blot analysis. Keywords: GPX 1, RT-PCR, Capsaicin, Diabetes, Liver, Rat Kapsaisin Uygulanan Diabetik Sıçanların Karaciğer Dokusunda GPX 1'in Real Time-PCR ile Expresyon Profilinin Çıkarılması ÖzetBu çalışmada, Kapsaisin (CAP) uygulanan, sağlıklı ve diabetik sıçanların karaciğer dokusunda Glutatyon Peroksidaz 1' in (GPX 1) RT-PCR ile belirlenmesi amaçlanmıştır. Araştırmada 20 adet Sprague-Dawley ırkı sıçan, Diyabet (Grup I), Sadece Kapsaisin Uygulanan (Grup II), Kapsaisin uygulanmış Diabetli (Grup III) ve Kontrol (Grup IV) olmak üzere dört gruba ayrıldı. Kapsaisin uygulamasına, streptozotosin (STZ) enjeksiyonundan 72 saat sonra başlanıp, 2 hafta boyunca hem diabetik gruba hem de sadece kapsaisin uygulanan gruba her gün 1 mg/kg kapsaisin, %10 ethanol, %1 Tween 20 ve %80 distile su ile çözdürüldükten sonra subkutan olarak uygulandı. Karaciğerdeki GPX 1 geninin ekspresyonu için yapılan RT-PCR analizi sonucunda; kapsaisin uygulanan diabet grubunda GPX 1'in gen ekspresyonunun kontrol grubu da dahil diğer gruplara göre daha yüksek olduğunu belirlendi (P<0.05). Sonuç olarak diabette kullanılan kapsaisinin GPX 1 transkripsiyon artışına neden olduğu ancak protein seviyesinde bir artışa yansıyıp yansımadığının, western-blot gibi metodlarla da belirlenmesi gerektiği sonucuna varıldı.

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Effects of sensorial denervation induced by capsaicin injection at birth or on day three of life, on puberty, induced ovulation and pregnancy

Cited by 27 publications

References 36 publications

Predominance of supraspinal innervation of the left ovary

Predominance of supraspinal innervation of the left ovary

Release of ovarian progesterone during the rat oestrous cycle by ganglionic cholinergic influence

Kapsaisin Uygulanan Diabetik Sıçanların Karaciğer Dokusunda GPX 1’in Real Time-PCR ile Expresyon Profilinin Çıkarılması

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