Release of ovarian progesterone during the rat oestrous cycle by ganglionic cholinergic influence

Sosa, Zulema; Delgado, Marcela Sánchez; Casais, Marilina; Aguado, Luis I.; Rastrilla, Ana María

doi:10.1016/j.jsbmb.2004.03.119

Cited by 21 publications

(27 citation statements)

References 30 publications

(41 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results are in agreement with those reported by Sosa et al (2004) for progesterone in Diestrus 1 when the predominant ovarian structure is the corpus luteum, and for progesterone and androstenedione in rats of 30 days of age . In both studies, they use the same system.…”

Section: Discussionsupporting

confidence: 93%

“…Likewise, Sosa et al (2004) demonstrated that the secretion of ovarian progesterone was not modified by the addition of the acetylcholine to the ganglion compartment on Diestrus 2 and pro-estrus when the corpora lutea start regression. However, the existence of neural output is demonstrated by the effect of the antagonists, mainly atropine, which is in general an indication of the system viability and particularly, of the participation of the ganglionic muscarinic receptors mainly at the level of the secondary interneurons.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Pregnancy in rats is modulated by ganglionic cholinergic action

Casais¹,

Delgado²,

Sosa³

et al. 2006

Reproduction

View full text Add to dashboard Cite

The control of ovarian steroidogenesis during pregnancy is mainly of endocrine origin. At present, there is little information about the influence of neural factors on the gestation physiology. The purpose of this work was to study the action of cholinergic agents in celiac ganglion upon the liberation of progesterone and ovarian androstenedione in the second half of pregnancy in rats. We used the ex vivo celiac ganglion-superior ovarian nerve-ovary integrated system (celiac ganglion-SON-ovary) that was incubated in buffer solution for 180 min, with the celiac ganglion and the ovary located in different compartments and linked by the SON.The results obtained indicate that the control values of ovarian androstenedione vary according to the pregnancy day analyzed. The addition of acetylcholine in ganglion decreased the liberation of both steroids on Day 15 whereas at the end of pregnancy it decreased the liberation of androstenedione without modifying progesterone. Due to the effect observed with atropine and hexametonium, acetylcholine action might occur through unspecific ganglionic pathways (Days 15 and 21) or through muscarinic ganglionic receptors (Days 19 and 20). Thus, we conclude that the cholinergic sympathetic system from the celiac ganglion might be a fine modulator of the pregnancy physiology.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Pregnancy in rats is modulated by ganglionic cholinergic action

Casais¹,

Delgado²,

Sosa³

et al. 2006

Reproduction

View full text Add to dashboard Cite

show abstract

“…If we assume that the coeliac ganglion-SON-ovary system constitutes a viable and functional entity with its own autonomic tone, we can use antagonists in coeliac ganglion without the simultaneous addition of the agonist as observed in other stages [8,26,39].…”

Section: Discussionmentioning

confidence: 99%

Ganglionic Adrenergic Action Modulates Ovarian Steroids and Nitric Oxide in Prepubertal Rat

et al. 2006

Self Cite

View full text Add to dashboard Cite

Abstract. Both peripheral innervation and nitric oxide (NO) participate in ovarian steroidogenesis. The purpose of this work was to analyse the ganglionic adrenergic influence on the ovarian release of steroids and NO and the possible steroids/ NO relationship. The experiments were carried out in the ex vivo coeliac ganglion-superior ovarian nerve (SON)-ovary system of prepubertal rats. The coeliac ganglion-SON-ovary system was incubated in Krebs Ringer-bicarbonate buffer in presence of adrenergic agents in the ganglionic compartment. The accumulation of progesterone, androstenedione, oestradiol and NO in the ovarian incubation liquid was measured. Norepinephrine in coeliac ganglion inhibited the liberation of progesterone and increased androstenedione, oestradiol and NO in ovary. The addition of α and β adrenergic antagonists also showed different responses in the liberation of the substances mentioned before, which, from a physiological point of view, reveals the presence of adrenergic receptors in coeliac ganglion. In relation to propranolol, it does not revert the effect of noradrenaline on the liberation of progesterone, which leads us to think that it might also have a "per se" effect on the ganglion, responsible for the ovarian response observed for progesterone. Finally, we can conclude that the ganglionic adrenergic action via SON participates on the regulation of the prepubertal ovary in one of two ways: either increasing the NO, a gaseous neurotransmitter with cytostatic characteristics, to favour the immature follicles to remain dormant or increasing the liberation of androstenedione and oestradiol, the steroids necessary for the beginning of the near first estral cycle. AT present, there is clear evidence that ovarian steroidogenesis, besides being regulated by the classic hypothalamo-hypophyseal-ovarian axis, is influenced by the neural pathway. The main neural information reaches the ovaries by the hilium via the superior ovarian nerve and ovarian plexus [1,2]. Hirshfield [3] confirmed that the first follicles to start growing are those assembled near the ovarian hilium, the first region in the ovaries of the rat to be innervated during its fetoneonatal life [4]. The sympathetic innervation terminals are closely related to the steroidogenic ovarian cells [5], and peptidergic and catecholaminergic neurotransmitters, mainly norepinephrine [6][7][8], are released from the superior ovarian nerve (SON) to the ovary. Both peptides and norepinephrine act via adenylate cyclase coupled receptors that are present in the ovary at the beginning of the folliculogenesis [9,10]. Several experimental designs have been used to investigate the role of innervation in the control of the ovarian function of prepubertal rat. In this respect, it has been reported in ovarian cell culture that the effect of adrenergic agents depends on the number of betareceptor sites present in the ovaries of pre and peripubertal rats [11].Experiments carried out by our group have shown that the bilateral transection of the neonatal SON ...

show abstract

“…The effects of catecholamines in the ovary have been demonstrated in other experimental schemes (Aguado & Ojeda 1984, Barria et al 1993. Sosa et al (2004) demonstrated in an ex vivo system that the cholinergic stimulus in the coeliac ganglion caused changes in NA and P 4 release at the ovarian level, according to the stage of the oestrous cycle, and this constitutes one of the factors governing the secretory activity of the ovarian steroids (Sosa et al 2004). Recent studies using the SMG-ONP-ovary system have determined an increase in NA release in the ovarian compartment on oestrus day by cholinergic ganglionic stimulus (A Vega Orozco, C Daneri, D Bronzi, Z Sosa, AM Rastrilla, unpublished observations, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…Our research group has demonstrated that the cholinergic agent in the coeliac ganglion stimulates NO release through NOS in prepubertal rat ovaries (Delgado et al 2004). Studies with antagonists of the neuronal and inducible NO enzyme, both at the ganglionic and ovarian levels, showed that NO can reach the ovary by neural ways or be synthesised at the ovarian level (Delgado et al (2006,2010) have demonstrated that NO is involved in the formation and maintenance of the corpus luteum during the oestrous cycle through the ONP pathway.…”

Section: Discussionmentioning

confidence: 99%

Involvement of the oestrogenic receptors in superior mesenteric ganglion on the ovarian steroidogenesis in rat

Orozco¹,

Daneri²,

Anesetti³

et al. 2012

Reproduction

Self Cite

View full text Add to dashboard Cite

Oestradiol (E 2 ) is a key hormone in the regulation of reproductive processes. The aims of this work were a) to examine the distributions of oestrogen receptor a (ERa) and ERb in the neurons of the superior mesenteric ganglion (SMG) in the oestrus stage by immunohistochemistry, b) to demonstrate whether E 2 in the SMG modifies progesterone (P 4 ), androstenedione (A 2 ) and nitrite release in the ovarian compartment on oestrus day and c) to demonstrate whether E 2 in the ganglion modifies the activity and gene expression in the ovary of the steroidogenic enzymes 3b-hydroxysteroid dehydrogenase (3b-HSD) and 20a-hydroxysteroid dehydrogenase (20a-HSD). The ex vivo SMG-ovarian nervous plexus-ovary system was used. E 2 , tamoxifen (Txf) and E 2 plus Txf were added in the ganglion to measure ovarian P 4 release, while E 2 alone was added to measure ovarian A 2 and nitrites release. Immunohistochemistry revealed cytoplasmic ERa immunoreactivity only in the neural somas in the SMG. E 2 increased ovarian P 4 and A 2 release at 15, 30 and 60 min but decreased nitrites. The activity and gene expression of 3b-HSD increased, while the activity and gene expression of 20a-HSD did not show changes with respect to the control. Txf in the ganglion diminished P 4 release only at 60 min. E 2 plus Txf in the ganglion reverted the effect of E 2 alone and the inhibitory effect of Txf. The results of this study demonstrate that ERa activation in the SMG has an impact on ovarian steroidogenesis in rats, thus providing evidence for the critical role of peripheral system neurons in the control of ovarian functions under normal and pathological conditions.

show abstract

Release of ovarian progesterone during the rat oestrous cycle by ganglionic cholinergic influence

Cited by 21 publications

References 30 publications

Pregnancy in rats is modulated by ganglionic cholinergic action

Pregnancy in rats is modulated by ganglionic cholinergic action

Ganglionic Adrenergic Action Modulates Ovarian Steroids and Nitric Oxide in Prepubertal Rat

Involvement of the oestrogenic receptors in superior mesenteric ganglion on the ovarian steroidogenesis in rat

Contact Info

Product

Resources

About