Effects of selenoprotein P on the contraction and relaxation of the airway smooth muscle

Abstract

Selenoprotein P (SeP) not only represents the major selenoprotein in plasma, but also provides more than 50% of the total plasma selenium. However, there is no report concerning the direct action of selenium or selenium-containing compounds on the contraction and relaxation of the airway smooth muscle. Therefore, we investigated the effects of SeP and sodium selenite (SS) on the indirectly induced contraction and relaxation of the cat bronchi, and gel contraction of cultured bovine tracheal smooth muscle cells (BTSMC) induced by ATP. In the present results, SeP or SS suppressed the amplitude of twitch-like contractions of cat bronchiole without affecting the non-adrenergic and non-cholinergic (NANC) relaxations evoked by electrical field stimulation. SeP also suppressed the ATP-induced gel contraction of BTSMC. These results suggest that SeP suppresses the amplitude of twitch-like contraction of cat bronchiole by acting directly on the bronchiolar smooth muscle.

Post-Translational Protein Deimination Signatures in Serum and Serum-Extracellular Vesicles of Bos taurus Reveal Immune, Anti-Pathogenic, Anti-Viral, Metabolic and Cancer-Related Pathways for Deimination

“…It has antioxidant properties [188] and serves in homeostasis and distribution of selenium [189]. Roles for selenoproteins in the regulation of the contraction and relaxation of bronchiolar smooth muscle have furthermore been identified [192]. Sepp1 is known to be glycosylated, and recently it has been identified as a deimination candidate in cetaceans, pinnipeds and alligators [93,96,97].…”

The role of selenium in cardiology

“…Однако не совсем понятно, каким образом в гладкой мышце легочных сосудов может синтезироваться такое количество SeP. Кроме того, заявление о том, что SeР вызывает митохондриальную дисфункцию и усугубляет оксидативный стресс, по нашему мнению, следует интерпретировать с осторожностью, поскольку оно не согласуется с общими представлениями об антиоксидантных свойствах SeР. В определенной степени выводам авторов об основной роли SeР в патогенезе ЛГ противоречат результаты исследований о влиянии SeР на сократительную реакцию изолированных сегментов бронхов кошки, где было показано, что добавление SeР вызывало их расслабление [41]. Несмотря на масштабность работы Kikuchi с соавт., вопрос о влиянии добавки селена больным ЛГ не рассматривался и требует отдельного изучения.…”

Potent cough suppression by physiologically active substance in human plasma

“…To date, however, the 25 identified human selenoproteins have not been associated with a function (Kryukov et al 2003). We recently discovered that selenoprotein P directly inhibits the twitch-like contraction of bronchial smooth muscle evoked by indirect muscle stimulation (Ogawa-Kitamura et al 2013).…”