Effects of Selenium on Antioxidant Activity and Recovery From Sciatic Nerve Ischemia–Reperfusion in Adult Rats

Abstract

Background: Selenium is an antioxidant trace element that is capable protects various tissues against damage induced by ischemiareperfusion injury. Objectives: This study examines the selenium effect on antioxidant activity and recovery from sciatic nerve ischemia-reperfusion in adult rats. Materials and Methods:In this experimental study, 80 adult male Wistar rats weighing 250 -300 g were used. They were divided into ten groups (n = 8 per group). The first group received sham surgery only. The second group received 0.2 mg/kg selenium without ischemiareperfusion surgery and eight remaining groups were divided to four control groups and four experimental groups which the latter groups received 0.2 mg/kg selenium. All ischemia groups were rendered in ischemic for 3 hours and reperfused for various times of 3, 7, 14 and 28 days. Half of the groups had experimental selenium (0.2 mg/kg) intraperitoneal injection treatment immediately after ischemia. According to a schedule for each group of rats bled and then centrifuged, and serum was prepared for assessment of activity levels of glutathione peroxidase (GPX), catalase (CAT), nitric oxide (NO), paraoxonase (POX), and malondialdehyde (MDA). Results: Comparison of the serum GPX, CAT, NO, POX, and MDA levels in the groups that received selenium with corresponding control group showed that selenium can increase plasma activity levels of GPX, POX and decrease activity levels of NO. Conclusions: Selenium with decreased activity levels of NO and increased activity GPX and POX can decrease ischemia-reperfusion injury in lower limb especially sciatic nerve.

Selenium Effect on Ischemia-Reperfusion Injury of Gastrocnemius Muscle in Adult Rats

“…These results are consistent with recent findings. Recently, Zendedel et al showed that selenium with decreased activity levels of NO and increased activity GPX and POX can decrease ischemia-reperfusion injury in hind limb of rat [17]. These results are consistent with recent findings.…”

“…Histological studies showed that selenium prevents ischemiareperfusion injury containing edema, tissue structure disorganization, and degeneration. Zendedel et al showed that selenium with decreased activity levels of NO and increased activity GPX and POX can decrease ischemia-reperfusion injury in hind limb especially sciatic nerve [17]. TNF-α is a factor involved in systemic inflammation and stimulate the acute phase reaction in inflammation [18].…”

Selenium effects on antioxidant and inflammatory indices in renal ischemia-reperfusion injury in rats

“…In various studied, other treatments including sitagliptin (21), curcumin (14), silymarin (22), and coenzyme Q10 (23) could exert protective effects on antioxidant defense system. A recent study also indicated that Se could improve the activities of antioxidant enzymes in vivo (24). Our results are similar to other researches which showed the beneficial effects of natural antioxidants on the antioxidant defense system.…”

A review: Nanofibrous scaffold in possible prevention and treatment of coronary artery disease

“…Hydrogels have been also demonstrated as a nanofibrous scaffold to treat atherosclerosis. Jin and colleagues reported the efficacy of mesenchymal stem cells conjugated with poly(lactide‐co‐ε‐caprolactone) nanofibers for adherence with the infarcted region of a rat MI model thereby, resulting in the dilation of the left ventricle and cell survival and differentiation into cardiomyocytes . They concluded that only the infarcted area was significantly reduced in the MSC‐NFs group compared with other groups .…”