Effects of Selenium and L-Carnitine on Oxidative Stress in Blood of Rat Induced by 2.45-GHz Radiation from Wireless Devices

Gümral, Nurhan; Nazıroğlu, Mustafa; Koyu, Ahmet; Öngel, Kurtuluş; Çelik, Ömer; Saygın, Mustafa; Kahriman, Mesud; Çalışkan, Sadettin; Kayan, Mustafa; Gençel, Osman; Flores-Arce, Manuel

doi:10.1007/s12011-009-8372-3

Cited by 38 publications

(28 citation statements)

References 35 publications

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“…In addition, serum CAT activity was decreased (p<0.05) by administration of Se-vitamin E combination (Tables 2 and 3). These observations are in agreement with results of the investigations indicating the activity of CAT affected by acetic acid induced colitis [3,34]. CAT, an essential peroxisomal hydrogen peroxide-consuming enzyme, also decreased the oxidative effects of H 2 O 2 , superoxide dismutase, and GSH-Px that may play an important role as a system scavenging oxidative radicals.…”

Section: Discussionsupporting

confidence: 91%

“…ROS cause reversible or irreversible damage to biological molecules including DNA, lipids, proteins, carbohydrates, or any nearby molecule causing a cascade of chain reactions resulting in cellular damage [1,2,4,33]; hence, the biomolecules may lose their physiological functions in the tissues. Damage of these cellular components by free radicals may play an important role in various disorders of skin [8,9], colon [1-8, 10, 17], brain [33], and liver [15,16,34]. In Data are presented in mean±SD *p<0.05 (statistically significant compared to control group); **p<0.01 (statistically significant compared to control group); ***p<0.05 (statistically significant compared to acetic acid group); ****p<0.01 (statistically significant compared to acetic acid group) Data are presented in mean±SD *p<0.05 (statistically significant compared to control group); **p<0.01 (statistically significant compared to control group); ***p<0.05 (statistically significant compared to acetic acid group); ****p<0.01 (statistically significant compared to acetic acid group) this study, macroscopic and microscopic damages were significantly increased (p<0.01) by intrarectal injection of acetic acid and decreased (p<0.05 and p<0.01, respectively) in group treated by Se-vitamin E (Table 1).…”

Section: Discussionmentioning

confidence: 99%

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Protective Effects of Selenium and Vitamin E Combination on Experimental Colitis in Blood Plasma and Colon of Rats

Bıtıren

Karakılçık

Zerin

et al. 2009

Biol Trace Elem Res

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Ulcerative colitis increases oxidative damage accompanied by production of free oxygen radicals. Selenium (Se) and vitamin E are two natural antioxidants. The present study was undertaken to investigate the possible protective role of Se and vitamin E combination in experimental colitis induced by acetic acid (AA) in rats. This study was carried out on three groups, namely the first (control), the second (experimental colitis group, 2 ml 5% acetic acid), and the third groups (2 ml 5% acetic acid, vitamin E (100 mg/kg body weight (bw)) plus Se (0.2 mg/kg bw)). The activities of catalase (CAT), prolidase (PRS), myeloperoxidase (MPO), total antioxidant capacity (TAC), total oxidant status (TOS), oxidative stress index (OSI), total thiol (T-SH) were determined in plasma and colon samples. Macroscopic and microscopic damages in colon were increased by AA treatment (p < 0.01 and p < 0.01, respectively), whereas they were decreased by selenium and vitamin E treatment (p < 0.05 and p < 0.01, respectively). The activities of CAT and PRS in the plasma and colon were significantly affected (p < 0.05 and p < 0.01) by treatment of AA, Se, and vitamin E. MPO activity in colon was increased (p < 0.01) by AA treatment and decreased (p < 0.05) by Se and vitamin E administration. The values of TOS and OSI in plasma were increased (p < 0.5) by AA. The TAC and T-SH in colon were decreased (p < 0.05) by AA and increased (p < 0.05) by Se and vitamin E. Based upon these results, Se and vitamin E may play an important role in preventive indication of the oxidative damage associated by acetic acid caused inflammation.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Protective Effects of Selenium and Vitamin E Combination on Experimental Colitis in Blood Plasma and Colon of Rats

Bıtıren

Karakılçık

Zerin

et al. 2009

Biol Trace Elem Res

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“…The effect of LC on superoxide anion radical scavenging and hydrogen peroxide scavenging seems comparable with alpha-tocopherol and trolox (Gülçin, 2006). L-carnitine seems to have more protective effects than selenium on the electromagnetic radiation-induced blood toxicity by inhibiting free radical supporting antioxidant redox system (Gumral et al, 2009).…”

Section: Discussionmentioning

confidence: 82%

Urinary excretion of L-carnitine, acetyl-L-carnitine, propionyl-L-carnitine and their antioxidant activities after single dose administration of L-carnitine in healthy subjects

Cao

Chen

Wang

et al. 2013

Braz. J. Pharm. Sci.

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The urine excretion of L-carnitine (LC), acetyl-L-carnitine (ALC) and propionyl-Lcarnitine (PLC) and their relations with the antioxidant activities are presently unknown. Liquid L-carnitine (2.0 g) was administered orally as a single dose in 12 healthy subjects. Urine concentrations of LC, ALC and PLC were detected by HPLC. Superoxide dismutase (SOD), total antioxidative capacity (T-AOC), malondialdehyde (MDA) and nitrogen monoxidum (NO) activities were measured by spectrophotometric methods. The 0~2 h, 2~4 h, 4~8 h, 8~12 h, 12~24 h excretion of LC was 53.13±31.36 μmol, 166.93±76.87 μmol, 219.92±76.30 μmol, 100.48±23.89 μmol, 72.07±25.77 μmol, respectively. The excretion of ALC was 29.70±14.43 μmol, 80.59±32.70 μmol, 109.85±49.21 μmol, 58.65±18.55 μmol, and 80.43±35.44 μmol, respectively. The urine concentration of PLC was 6.63±4.50 μmol, 15.33±12.59 μmol, 15.46±6.26 μmol, 13.41±11.66 μmol and 9.67±7.92 μmol, respectively. The accumulated excretion rate of LC was 6.1% within 24h after its administration. There was also an increase in urine concentrations of SOD and T-AOC, and a decrease in NO and MDA. A positive correlation was found between urine concentrations of LC and SOD (r = 0.8277) or T-AOC (r = 0.9547), and a negative correlation was found between urine LC excretions and NO (r = -0.8575) or MDA (r = 0.7085). In conclusion, a single oral LC administration let to a gradual increase in urine L-carnitine excretion which was associated with an increase in urine antioxidant enzymes and the total antioxidant capacities. These data may be useful in designing therapeutic regimens of LC or its analogues in the future.Uniterms: L-carnitine/antioxidant activity. Acetyl-L-carnitine/antioxidant activity. Propionyl-Lcarnitine/ antioxidant activity. Antioxidants. Urine excretion/analysis. A excreção urinária de L-carnitina (LC), acetil-L-carnitina (ALC) e propionil-L-carnitine (PLC) e as suas relações com as atividades antioxidantes são presentemente desconhecidos. Líquido de L-carnitina (2,0 g) foi administrada por via oral como uma dose única em 12 indivíduos saudáveis. INTRODUCTIONL-carnitine (3-hydroxy-4-N-trimethylammonium butyrate, LC) is an endogenous compound which has several physiological functions. LC is involved in the transfer of long-chain fatty acids across the inner matrix membrane of mitochondria (Mancinelli et al., 2007). It also regulates acetyl storage and transfer in mitochondria, cells, and between organs, and the transport of potentially toxic, activated acids out of mitochondria (Bellinghieri et al., 2003). LC is believed to be important for acting as an osmo protectant in organs such as the kidney, and as a general cell membrane stabilizer (Lahjouji et al., 2004;Biolo et al., 2008). LC homeostasis is maintained by a modest biosynthesis in the liver and kidney, absorption from dietary sources (eg, meat and dairy products), and efficient renal tubular reabsorption from glomerular filtrate (Rebouche, Seim 1998). Short-chain carnitine esters, including acetylLcarnitine (ALC) and propionyl-L-carni...

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“…Erythrocyte reduced GSH activities while erythrocyte GSH-PX activities in EMR group were found to be lower than in EMR+L-carnitine group. The frequency of 2450 MHz induced blood toxicity by inhibiting free radicals supporting antioxidant redox system although selenium has no effect on the investigated values (43). Da Silva et al aimed to assess antioxidant effects of MLT on the sickle cell suspension.…”

Section: Tab 3 the Effects Of Melatonin And 2450 Mhz Emr On Malondimentioning

confidence: 99%

The effects of electromagnetic radiation (2450 MHz wireless devices) on the heart and blood tissue: role of melatonin

Gümral¹,

Saygın²,

Aşçı³

et al. 2017

BLL

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OBJECTIVE: This study was designed to investigate the effects of 2450 MHz EMR on the heart and blood in rat and possible ameliorating effects of melatonin. MATERIAL AND METHOD: Thirty-two female Wistar Albino rats were randomly grouped (by eight in each group) as follows: Group I: cage-control group (dimethysulfoxide (DMSO), 10mg/kg/day i.p. without stress and EMR. Group II: sham-control rats stayed in restrainer without EMR and DMSO (10mg/kg/day i.p.). Group III: rats exposed to 2450 MHz EMR. Group IV: treated group rats exposed to 2450 MHz EMR+melatonin (MLT) (10mg/kg/day i.p.). RESULTS: In the blood tissue, there was no signifi cant difference between the groups in respect of erythrocytes GSH, GSH-Px activity, plasma LP level and vitamin A concentration (p > 0.05). However, in the Group IV, erythrocytes' LP levels (p < 0.05) were observed to be signifi cantly decreased while plasma vitamin C, and vitamin E concentrations (p < 0.05) were found to be increased when compared to Group III. In the heart tissues, MDA and NO levels signifi cantly increased in group III compared with groups I and II (p < 0.05). Contrary to these oxidant levels, CAT and SOD enzyme activities decreased signifi cantly in group III compared with groups I and II (p < 0.05) and increased in group IV compared with group I, however not signifi cantly (p > 0.05). Besides, MLT treatment lowered the MDA and NO levels compared with group III. DISCUSSION: In conclusion, these results demonstrated that contrary to its effect on the heart, the wireless (2450 MHz) devices cause slight oxidative-antioxidative changes in the blood of rats, and a moderate melatonin supplementation may play an important role in the antioxidant system (plasma vitamin C and vitamin E). However, further investigations are required to clarify the mechanism of action of the applied 2450 MHz EMR exposure (Tab. 3, Fig. 1, Ref. 49). Text in PDF www.elis.sk. KEY WORDS: 2450 MHz electromagnetic radiation, heart and blood of rat, oxidant-antioxidant system, melatonin.

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Effects of Selenium and L-Carnitine on Oxidative Stress in Blood of Rat Induced by 2.45-GHz Radiation from Wireless Devices

Cited by 38 publications

References 35 publications

Protective Effects of Selenium and Vitamin E Combination on Experimental Colitis in Blood Plasma and Colon of Rats

Protective Effects of Selenium and Vitamin E Combination on Experimental Colitis in Blood Plasma and Colon of Rats

Urinary excretion of L-carnitine, acetyl-L-carnitine, propionyl-L-carnitine and their antioxidant activities after single dose administration of L-carnitine in healthy subjects

The effects of electromagnetic radiation (2450 MHz wireless devices) on the heart and blood tissue: role of melatonin

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