Protective Effects of Selenium and Vitamin E Combination on Experimental Colitis in Blood Plasma and Colon of Rats

Abstract

Ulcerative colitis increases oxidative damage accompanied by production of free oxygen radicals. Selenium (Se) and vitamin E are two natural antioxidants. The present study was undertaken to investigate the possible protective role of Se and vitamin E combination in experimental colitis induced by acetic acid (AA) in rats. This study was carried out on three groups, namely the first (control), the second (experimental colitis group, 2 ml 5% acetic acid), and the third groups (2 ml 5% acetic acid, vitamin E (100 mg/kg body weight (bw)) plus Se (0.2 mg/kg bw)). The activities of catalase (CAT), prolidase (PRS), myeloperoxidase (MPO), total antioxidant capacity (TAC), total oxidant status (TOS), oxidative stress index (OSI), total thiol (T-SH) were determined in plasma and colon samples. Macroscopic and microscopic damages in colon were increased by AA treatment (p < 0.01 and p < 0.01, respectively), whereas they were decreased by selenium and vitamin E treatment (p < 0.05 and p < 0.01, respectively). The activities of CAT and PRS in the plasma and colon were significantly affected (p < 0.05 and p < 0.01) by treatment of AA, Se, and vitamin E. MPO activity in colon was increased (p < 0.01) by AA treatment and decreased (p < 0.05) by Se and vitamin E administration. The values of TOS and OSI in plasma were increased (p < 0.5) by AA. The TAC and T-SH in colon were decreased (p < 0.05) by AA and increased (p < 0.05) by Se and vitamin E. Based upon these results, Se and vitamin E may play an important role in preventive indication of the oxidative damage associated by acetic acid caused inflammation.

Iloprost reduces colitis induced oxidative stress: An experimental study in rats

“…This fact makes this study difficult to differentiate from a classical ischemia type presentation, versus a colitis presentation. The AA induced colitis model resembles UC in humans in terms of the histopathological appearance, due in part because of the over production of oxidant radicals (26,27). Most UC patients have rectal involvement, and UC also tends to occur on the rectal mucosa initially, and subsequently progresses retrograde to the other parts of the colon.…”

Melatonin Expresses Powerful Anti-inflammatory and Antioxidant Activities Resulting in Complete Improvement of Acetic-Acid-Induced Colitis in Rats

“…Free radicals play an important role in the pathogenesis of the mucosal injuries [23,24]. The AA-induced colitis model resembles UC in humans in terms of histopathological appearance by causing overproduction of oxidant radicals that play pivotal roles in the pathophysiology [25], and produces excess reactive oxygen species that cause damage to biological molecules in any part of the cellular components [25,26]. In previous studies, free radicals and reactive oxygen species in colonic mucosa were described in colorectal specimens of UC [25,27].…”

Effect of L-carnitine on serum paraoxonase, arylesterase and lactonase activities and oxidative status in experimental colitis

“…Çalışmamızda da L-karnitin tedavisi kolon dokularında makroskopik skoru değiştirmedi. Ayrıca L-karnitin uygulanan gruplarda mikroskopik skorlarda azalma olmasına rağmen bu azalma istatistik- Asetik asit ile oluşturulan deneysel kolit modelinde serum TOS düzeylerinin yükseldiği gösterilmiştir [33,34]. Literatürle uyumlu olarak kolit grubunun serum TOS düzeylerini kontrol grubuna göre anlamlı olarak yük-sek bulduk.…”

“…Bitiren ve ark. [34] ise aynı modelde serum TAS düzey-lerinin azaldığını bildirmişlerdir. Biz de deneysel kolit modeli oluşturulan sıçanların serum TAS düzeylerini kontrol grubundan anlamlı olarak düşük bulduk.…”