Effects of Sea Salts on Induction of Cell Proliferation in Liquid Cultures of Mangrove Plants, Sonneratia caseolaris and S. alba

Yamamoto, Raiki; Kawana, Yoshifumi; Minagawa, Reiko; Sasamoto, Hamako

doi:10.4236/ajps.2011.21004

Cited by 10 publications

(9 citation statements)

References 15 publications

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“…The major constituent, Fe, was spread over the surface uniformly, while the components of salt compounds were Na, Mg, and Cl from seawater. The presence of Ca kations was indicated by CaCl 2 compound in seawater [20]. In addition, oxygen covered the surface, which denotes corrosion products of oxide film.…”

Section: Element Distribution Of Corroded Surface After Exposurementioning

confidence: 97%

Corrosion Behavior of Mild Steel in Seawater from Northern Coast of Java and Southern Coast of Bali, Indonesia

Sundjono

Priyotomo

Nuraini

et al. 2018

J. Eng. Technol. Sci.

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Abstract. The corrosion behavior of mild steel in seawater was investigated using an immersion test and energy-dispersive X-ray spectroscopy in solutions from the sites of Muara Baru, Suramadu and Tol Mandara. As reference, solutions of 3.5% and 5% NaCl were prepared. Dissolved oxygen, salinity, conductivity, and total dissolved solids (TDS) were measured. It was found that uniform corrosion occurred on the mild steel during the immersion test. The corrosion resistance of the steel decreased with increasing exposure time. Since the magnitudes of conductivity, salinity and TDS of all test solutions were similar, it was concluded that dissolved oxygen (DO) and chloride ions play an essential role in inducing the corrosion risk of mild steel. Both parameters hinder the formation of a stable passive film on the surface of corroded mild steel.

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Section: Element Distribution Of Corroded Surface After Exposurementioning

confidence: 97%

Corrosion Behavior of Mild Steel in Seawater from Northern Coast of Java and Southern Coast of Bali, Indonesia

Sundjono

Priyotomo

Nuraini

et al. 2018

J. Eng. Technol. Sci.

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“…Strong salt tolerance at the cellular level of several mangrove plants, growing in the coastal region with a high salinity, has been found using tissue cultured cells and protoplasts (Kawana & Sasamoto, 2008;Hayashi et al, 2009;Yamamoto et al, 2011;Hasegawa et al, 2013).…”

Section: Salt Tolerance and Allelopathy Of Mangrove Cultured Cellsmentioning

confidence: 99%

Evaluation of an Anthocyanin, Cyanidin 3,5-di-O-glucoside, as an Allelochemical in Red Callus of a Mangrove Sonneratia ovata, Using Protoplast Co-Culture Bioassay Method with Digital Image Analysis

Sasamoto

Iwashina

Suzuki

et al. 2018

JPS

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Protoplasts isolated from red callus of a mangrove, Sonneratia ovata were co-cultured with recipient lettuce protoplasts to examine the allelopathic activities. Protoplasts were isolated with Cellulase R10 and Driselase 20 in 0.6 M mannitol solution and purified by density gradient centrifugation on 0.6 M sucrose. Protoplasts were co-cultured in 50 μL of liquid Murashige and Skoog's (MS) basal medium containing 1 μM 2,4-dichlorophenoxyacetic acid and 0.1 μM benzyladenine and 0.6 M mannitol solution in a 96-well culture plate. Protoplast density ranged from 5 × 10 3 /mL to 10 5 /mL. Cell division of lettuce protoplasts was strongly inhibited by addition of S. ovata protoplasts, and non-spherical cell enlargement was slightly inhibited. By contrast, digital image analysis of scanned 96-well culture plates revealed no inhibition in accumulation of yellow color in lettuce protoplasts. An anthocyanin, cyanidin 3,5-di-O-glucoside (cyanin), was identified and its content in the red callus was ca. 1 mM of fresh weight. The effects of cyanin on the growth of lettuce protoplasts at three stages were similar to those of red S. ovata protoplasts. From these results, cyanin was most likely the allelochemical contained in red callus of S. ovata. The allelopathic activity of cyanin was compared with that of other putative allelochemicals in several plant materials, using the protoplast co-culture method with digital image analysis.

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“… or 4 were replaced by amino acids [6,7]. Using cultured cells of three mangrove species, A. alba, B. sexangula and S. alba, the effects of sea salts on their growth were investigated and tolerance or halophilic nature were found [10,11].…”

Section: Nomentioning

confidence: 99%

“…In Sonneratiaceae, callus cultures have been obtained using pistils as explants [4] and suspension cells have successfully been obtained from cotyledons [5] of Sonneratia alba. In S. caseolaris, suspension cells were successfully generated from seedlings [6]. These cultures were maintained in the Murashige & Skoog's (MS) basal medium [7], which is commonly used in tissue cultures of not only herbaceous plants but also in woody plants.…”

Section: Introductionmentioning

confidence: 99%

Relation between Amino Acids Profiles and Recalcitrancy of Cell Growth or Salt Tolerance in Tissue and Protoplast Cultures of Three Mangrove Species, Avicennia alba, Bruguiera sexangula, and Sonneratia alba

Tsuchiya¹,

Ogita²,

Kawana³

et al. 2013

AJPS

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Amino acids profiles were investigated in tissues, cultured cells, i.e. callus or suspension cells, and their protoplasts of three mangrove species, Avicennia alba, Bruguiera sexangula, and Sonneratia alba. Original tissues of cultured cells of three mangrove species were cotyledons and hypocotyls, leaves, and cotyledons, respectively. In protoplasts isolated from cultured cells, glutamine and alanine were the major amino acids. Different contents of glycine, proline and serine were observed among protoplasts of three mangrove species. Large differences in the major amino acids were found among cultured cells and their protoplasts while no difference was found between callus and suspension cells independent of additional salt in culture medium. Protoplasts of original tissues, young leaves and cotyledons, contained alanine and glutamine and/or asparagine. In suspension cells of B. sexangula, total contents of amino acids were low while their protoplasts showed similar value as of other samples. Protoplasts of leaf and cotyledons of A. alba and cotyledons of A. lanata, A. marina and S. alba were also investigated. The total contents of amino acids and their profiles might be related to the recalcitrance for the growth and salt tolerance or halophilic nature of cells and basal media used for the maintenance of cell cultures or protoplast cultures of the mangrove species. This is the first report on callus induction from hypocotyls of A. alba.

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Effects of Sea Salts on Induction of Cell Proliferation in Liquid Cultures of Mangrove Plants, Sonneratia caseolaris and S. alba

Abstract: ABSTRACT

Cited by 10 publications

References 15 publications

Corrosion Behavior of Mild Steel in Seawater from Northern Coast of Java and Southern Coast of Bali, Indonesia

Corrosion Behavior of Mild Steel in Seawater from Northern Coast of Java and Southern Coast of Bali, Indonesia

Evaluation of an Anthocyanin, Cyanidin 3,5-di-O-glucoside, as an Allelochemical in Red Callus of a Mangrove Sonneratia ovata, Using Protoplast Co-Culture Bioassay Method with Digital Image Analysis

Relation between Amino Acids Profiles and Recalcitrancy of Cell Growth or Salt Tolerance in Tissue and Protoplast Cultures of Three Mangrove Species, <i>Avicennia alba</i>, <i>Bruguiera sexangula</i>, and <i>Sonneratia alba</i>

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Effects of Sea Salts on Induction of Cell Proliferation in Liquid Cultures of Mangrove Plants, Sonneratia caseolaris and S. alba

Abstract: ABSTRACT

Cited by 10 publications

References 15 publications

Corrosion Behavior of Mild Steel in Seawater from Northern Coast of Java and Southern Coast of Bali, Indonesia

Corrosion Behavior of Mild Steel in Seawater from Northern Coast of Java and Southern Coast of Bali, Indonesia

Evaluation of an Anthocyanin, Cyanidin 3,5-di-O-glucoside, as an Allelochemical in Red Callus of a Mangrove Sonneratia ovata, Using Protoplast Co-Culture Bioassay Method with Digital Image Analysis

Relation between Amino Acids Profiles and Recalcitrancy of Cell Growth or Salt Tolerance in Tissue and Protoplast Cultures of Three Mangrove Species, &lt;i&gt;Avicennia alba&lt;/i&gt;, &lt;i&gt;Bruguiera sexangula&lt;/i&gt;, and &lt;i&gt;Sonneratia alba&lt;/i&gt;

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Relation between Amino Acids Profiles and Recalcitrancy of Cell Growth or Salt Tolerance in Tissue and Protoplast Cultures of Three Mangrove Species, <i>Avicennia alba</i>, <i>Bruguiera sexangula</i>, and <i>Sonneratia alba</i>