Effects of salt and heat pre-treatment factors on efficient regeneration in barley (Hordeum vulgare L.)

Haque, Ziaul; Islam, S. M. Shahinul; Subramaniam, Sreeramanan

doi:10.1007/s13205-017-0675-z

Cited by 11 publications

(6 citation statements)

References 39 publications

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“…In vitro culture, a tissue culture approach is frequently identified as a salt-tolerant crop and was employed to find salt-tolerant cell lines of rice (30,31), barley (32), potato (7,33), sugarcane (34) and tomato (35). Key criteria for success in the in vitro selection technique for salt tolerance are believed to be a large variety of cells, easy salt administration and an in vitro selection approach for the tolerant cell.…”

Section: Introductionmentioning

confidence: 99%

Hydroponic and in vitro screening of wheat varieties for salt-tolerance

et al. 2022

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Salt-tolerant wheat cultivars are essential for sustainable wheat production and global food security. The present study aimed to establish a reliable screening protocol as well as successfully isolated the potential salt-tolerant wheat varieties by discerning morpho-physiological parameters with multivariate analysis. Seventeen wheat varieties were evaluated at 0, 12, 15 and 18 dSm-1 salinity levels in a hydroponic culture system at the seedling stage. Moreover, in vitro callusing responses of four selected varieties were determined to clarify the salt tolerance capability at 0, 9, 12 and 15 dSm-1 salt treatments. The seedling growth of most wheat varieties was highly interrupted and reduced by the toxic effects of salinity, however, some varieties such as BARI Gom-32, BARI Gom-33, BARI Gom-31, BARI Gom-30, and BARI Gom-28 showed the lowest reduction under all salinity stress conditions. The total salt tolerance index (TSTI) showed that the cultivar BARI Gom-33 was the most salt-tolerant followed by BARI Gom-32 and BARI Gom-30 whereas BARI Gom-25 was identified as the most sensitive. These results were strongly supported by the principal component analysis (PCA) and Ward’s Methods Euclidean based clustering. In vitro results revealed that the lowest reduction of callus induction was recorded in BARI Gom-33 which might show the greatest tolerance to salinity by improving morpho-physiological characteristics against salt stress. Therefore, the identified genotypes might be employed as donor parents to develop salt-tolerant and high-yielding cultivars in the wheat breeding programme.

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Section: Introductionmentioning

confidence: 99%

Hydroponic and in vitro screening of wheat varieties for salt-tolerance

et al. 2022

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“…5. This is due to the reduction of water availability and loss of turgor pressure (TP) in callus cells (Haque et al, 2017 The concentration of PEG 6000 that resulted in 50% decrease in cell viability and RGR (4% of PEG) was selected as sub-lethal concentration for Daher-Aljabal and Baskanta varieties while it was (6%) for Brieh variety and two sub-lethal concentrations (6% and 8% of PEG) were selected for Daraa variety.…”

Section: Resultsmentioning

confidence: 99%

Somaclonal Variation Selection for Tolerance to PEG-Induced Drought Stress in Four Local Tomato (Solanum lycopersicum L.) Varieties

Naddaf¹

2020

SJKFU

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Growth and development of tomato is restricted by numerous abiotic stress factors especially drought. Crop genetic improvement through traditional methods is time-consuming and conducted under uncontrolled conditions; thus, in vitro selection offers an efficient alternative. This research was carried out at the National Commission for Biotechnology (NCBT) and the General Commission for Scientific Agricultural Research (GCSAR), Syria during 2018 and 2019. The main objective was to select callus tolerant to drought stress and regenerate tolerant somaclonal variant plants in four local tomato varieties. Callus (formed from root explants on MS medium supplemented with 2 mg/L BAP and 0.2 mg/L NAA) was subjected to four different levels of drought stress induced by PEG 6000 (2%, 4%, 6%, 8% in addition to the non-treated control). After 8 weeks, cell viability, relative growth rate (RGR), proline content, and tolerance index (TI) were determined. Callus was then transferred to regeneration medium (MS supplemented with 2 mg/L zeatin, 0.2 mg/L GA3 and the sub-lethal concentration of PEG (4% for Daher-Aljabal, 6% for Brieh and 6%, 8% for Daraa variety). The regenerated somaclonal variant plants were evaluated, based on morphological characters, for drought tolerance compared to mother plant. Results showed that RGR, TI and cell viability decreased while free proline content increased in response to increasing PEG concentration. Based on the studied parameters, Daraa was the most tolerant variety, followed by Daher-Aljabal, Brieh and Baskanta. A single regenerated plantlet was obtained from Daher-Aljabal variety named DH1 and another plantlet from Brieh variety named BR1, while two regenerated plantlets were obtained from Daraa variety named DA1 and DA2. All of them showed better tolerance to drought stress than mother plants at 8% PEG concentration in terms of root length, plant height, root-shoot ratio and dry weight. In conclusion, a successful callus formation and plant regeneration protocol has been developed under drought stress treatments induced by PEG 6000. Four somaclonal variant plants were successfully developed and they were different from mother plants based on the studied parameters.

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“…Usually, stress increases the regeneration efficiency in plant tissue culture. For example, warm temperature promotes shoot regeneration in A. thaliana through the enhanced expression of several regenerationassociated genes, such as CUP-SHAPED COTYLEDON 1 (CUC1) encoding a transcription factor involved in shoot meristem formation and YUCCAs (YUCs) encoding auxin biosynthesis enzymes [40,41]. In summary, the effects of temperature on plant tissue culture can be complex and multifaceted.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Regeneration of Chrysanthemum from Ovaries and Ovules Treated with Thermal and Chemical Stimuli: Morphogenic and Cytogenetic Effects

Miler,

Tymoszuk,

Rewers

et al. 2023

Agriculture

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Chrysanthemum (Chrysanthemum × morifolium (Ramat.) Hemsl.) holds a prominent position in the market of ornamental plants. To further advance chrysanthemum breeding efforts, the development of haploids may be useful. Therefore, the effect of various chemical and thermal treatments on regeneration efficiency and ploidy level in chrysanthemum was studied. Ovaries and ovules of three chrysanthemum cultivars, i.e., ‘Brasil,’ ‘Capitola,’ and ‘Jewel Time Yellow,’ were cultured either on a medium with 1 mg·L−1 2,4-dichlorophenoxyacetic acid (2,4-D) and different concentrations (0.5–1.5 mg·L−1) of thidiazuron (TDZ) or subjected to thermal shock (pretreatment temperature of 4 °C or 32 °C) and cultured on a medium with 1 mg·L−1 2,4-D and 1 mg·L−1 6-benzylaminopurine (BAP). It was found that ovaries had a greater organogenic potential (both in terms of callogenesis and shoot formation) than ovules. Microscopic analyses revealed that shoots mainly developed via indirect somatic embryogenesis from a callus developed from the ovary wall. The highest number of shoots was produced in cooled (at 4 °C) ovaries of chrysanthemum ‘Brasil’ and in ‘Jewel Time Yellow’ ovaries cultured on a medium with 1.0–1.5 mg·L−1 TDZ. The latter cultivar also had the highest potential to produce plants with an altered ploidy level (doubled and halved the number of chromosomes). This study demonstrates that manipulating factors such as temperature and thidiazuron concentration can enhance regeneration efficiency and induce altered ploidy levels in selected cultivars, offering valuable insights for chrysanthemum breeding programs.

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Effects of salt and heat pre-treatment factors on efficient regeneration in barley (Hordeum vulgare L.)

Cited by 11 publications

References 39 publications

Hydroponic and in vitro screening of wheat varieties for salt-tolerance

Hydroponic and in vitro screening of wheat varieties for salt-tolerance

Somaclonal Variation Selection for Tolerance to PEG-Induced Drought Stress in Four Local Tomato (Solanum lycopersicum L.) Varieties

In Vitro Regeneration of Chrysanthemum from Ovaries and Ovules Treated with Thermal and Chemical Stimuli: Morphogenic and Cytogenetic Effects

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