Effects of Salmonella enterica subsp. enterica serovar Enteritidis vaccination in layer hens subjected to S. Enteritidis challenge and various feed withdrawal regimens

Piao, Zhenzi; Toyota-Hanatani, Yukiko; Ohta, Hiroaki; Sasai, Kazumi; Tani, Hiroyuki; Baba, Eiichiroh

doi:10.1016/j.vetmic.2007.05.008

Cited by 15 publications

(8 citation statements)

References 21 publications

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“…Those authors observed similar results when working with 30-week-old chickens challenged with the same bacterial strain (Sadeyen et al, 2006). Another study testing layer hens challenged with S. Enteritidis Y-24 (phage type 4) showed that the highest colonization in the caecum was achieved on days 3 and 4 post-challenge (Piao et al, 2007). As the maximum colonization happened by 1 week, we believed that it was important to monitor the window between days 1 and 7 post-challenge.…”

Section: Discussionsupporting

confidence: 59%

Salmonella enterica subspecies enterica serovar Enteritidis Salmonella pathogenicity island 2 type III secretion system: role in intestinal colonization of chickens and systemic spread

et al. 2010

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Salmonella enterica subspecies enterica serovar Enteritidis (S. Enteritidis) has been identified as a significant cause of salmonellosis in humans. Salmonella pathogenicity islands 1 and 2 (SPI-1 and SPI-2) each encode a specialized type III secretion system (T3SS) that enables Salmonella to manipulate host cells at various stages of the invasion/infection process. For the purposes of our studies we used a chicken isolate of S. Enteritidis (Sal18). In one study, we orally co-challenged 35-day-old specific pathogen-free (SPF) chickens with two bacterial strains per group. The control group received two versions of the wild-type strain Sal18: Sal18 attTn7 : : tet and Sal18 attTn7 : : cat, while the other two groups received the wild-type strain (Sal18 attTn7 : : tet) and one of two mutant strains. From this study, we concluded that S. Enteritidis strains deficient in the SPI-1 and SPI-2 systems were outcompeted by the wild-type strain. In a second study, groups of SPF chickens were challenged at 1 week of age with four different strains: the wild-type strain, and three other strains lacking either one or both of the SPI-1 and SPI-2 regions. On days 1 and 2 post-challenge, we observed a reduced systemic spread of the SPI-2 mutants, but by day 3, the systemic distribution levels of the mutants matched that of the wild-type strain. Based on these two studies, we conclude that the S. Enteritidis SPI-2 T3SS facilitates invasion and systemic spread in chickens, although alternative mechanisms for these processes appear to exist.

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Section: Discussionsupporting

confidence: 59%

Salmonella enterica subspecies enterica serovar Enteritidis Salmonella pathogenicity island 2 type III secretion system: role in intestinal colonization of chickens and systemic spread

et al. 2010

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“…For all analysis times, SH counts in the intestines exceeded those in the viscera. These results demonstrate the challenge faced by the poultry sector in attempting to control SH, and the risks related to positive testing in poultry products, since this microorganism is present in considerable quantities in both the intestine and the viscera (Piao et al, 2007;Wollin, 2007;Gantois et al, 2008). The SH in Brazil has asymptomatic manifestation in positive birds and its presence offers a risk just for humans.…”

Section: Introductionmentioning

confidence: 76%

Evaluation of the effectiveness and safety of a genetically modified live vaccine in broilers challenged with Salmonella Heidelberg

Muñíz¹,

Verdi²,

Leão³

et al. 2017

Avian Pathology

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Salmonellosis ranks among the major diseases of commercial poultry, and its presence in poultry flocks is responsible for economic losses and risks related to public health. Vaccines are an important tool within integrated programmes to control salmonellosis. The purpose of this study was to assess cross-protection provided by the Poulvac® ST vaccine in the control of Salmonella Heidelberg in experimentally challenged 3-and 21-day-old birds. Eighty birds were identified and separated into four treatments (T1: vaccinated and challenged at 3 days of age, T2: unvaccinated and challenged at 3 days of age, T3: vaccinated and challenged at 21 days of age, and T4: unvaccinated and challenged at 21 days of age). The inoculum was produced from a Brazilian field strain of SH. At the end of the experiment, caecum and liver/ spleen samples were collected for quantitative and qualitative analysis of SH, respectively. Analysis of the liver/spleen showed that Poulvac® ST significantly (P ≤ 0.05) reduced the percentage of SH positivity in the group challenged at 3 days of age, while in the group challenged at 21 days this difference was almost considered significant (P = 0.1818). On the other hand, there was no statistically significant difference in SH count in the caecum (CFU/g) in the group challenged at 3 days, but for the group challenged at 21 days the SH counts were significantly (P ≤ 0.05) lower in the vaccinated group when compared to the positive control.ARTICLE HISTORY

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“…Most of the studies pertaining to using of bacterins have investigated post-vaccine S. Enteritidis shedding over time (Woodward et al, 2003;Piao et al 2007). Only a few have looked at the immune response following vaccination, and these studies were limited to young chickens (less than four weeks old) (Liu et al, 2001;Babu et al, 2004;Okamura et al, 2004).…”

Section: Results Listed In Tablementioning

confidence: 99%

A Trial to Prevent Salmonella Enteritidis Infection in Broiler Chickens Using Autogenous Bacterin Compared with Probiotic Preparation

El-Ghany¹,

El-Shafii²,

Hatem³

et al. 2012

JAS

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This study was carried out to investigate the efficacy of the locally prepared autogenous Salmonella Enteritidis (S. Enteritidis) bacterin as well as a probiotic preparation in the prevention of broiler chickens from S. Enteritidis infection. A total of three hundred and ten, one day-old Hubbard broiler chicks were used. At day old, ten chicks were sacrificed and examined bacteriologically to prove their freedom from S. Enteritidis infection. Three hundred birds were divided into four equal groups. Chickens in group (1) were kept as blank control negative non infected-non treated birds, while those of group (2) were challenged non treated birds. Group (3) was vaccinated intramuscularly by the autogenous bacterin at the first day of age in a dose of 0.2 ml/bird and boostered as a second dose at 10 days of age in a dose 0.5 ml/bird, however, group (4) was given a commercial probiotic preparation as 1gm/ 4 liter of the drinking water from the first day of age and continued for 5 successive days. All birds in groups 2, 3, and 4 were challenged orally by 0.5 ml containing 10 9 CFU/ml S. Enteritidis at 20 days of age. All the groups were kept under complete observation for three weeks for recording signs, moralities, gross lesions, shedding rate of S. Enteritidis, re-isolation of the organism, the performance as well as detection of the titer of antibodies serologically using microagglutination test and enzyme linked immunosorbant assay (ELISA) test. The results showed that the both the bacterin and the probiotic are equally effective in reducing signs, mortalities, gross lesions, the shedding rate and the re-isolation of S. Enteritidis and also increasing in the performance of chickens. The effect of the bacterin and the probiotic was significant (P≤0.05) when compared with the infected non treated chickens. Moreover, the serological investigation revealed an improvement in the titer of antibodies after vaccination and probiotic treatment. In conclusion, double doses of locally prepared autogenous S. Enteritidis bacterin and the probiotic preparation were effective and safe methods for prevention of S. Enteritidis infection in broiler chickens.

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Effects of Salmonella enterica subsp. enterica serovar Enteritidis vaccination in layer hens subjected to S. Enteritidis challenge and various feed withdrawal regimens

Cited by 15 publications

References 21 publications

Salmonella enterica subspecies enterica serovar Enteritidis Salmonella pathogenicity island 2 type III secretion system: role in intestinal colonization of chickens and systemic spread

Salmonella enterica subspecies enterica serovar Enteritidis Salmonella pathogenicity island 2 type III secretion system: role in intestinal colonization of chickens and systemic spread

Evaluation of the effectiveness and safety of a genetically modified live vaccine in broilers challenged with Salmonella Heidelberg

A Trial to Prevent Salmonella Enteritidis Infection in Broiler Chickens Using Autogenous Bacterin Compared with Probiotic Preparation

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