Effects of Rutin on Wound Healing in Hyperglycemic Rats

Abstract

Long-term poor glycemic control negatively affects macrovascular and microvascular diseases, as well as wound restoration. Buckwheat is a good source of rutin (quercetin-3-O-rutoside) and has benefits in regulating blood sugar. This study was to evaluate the antioxidant and anti-inflammatory effects of rutin on wound healing in streptozotocin-induced hyperglycemic rats. Eighteen male Wistar rats were randomly divided into three groups: normal (NDM), hyperglycemic (DM), and hyperglycemic with rutin (DMR). After induction of hyperglycemia for 2 days, a 15 × 15 mm wound was induced on the back of each rat. Intraperitoneal injection of rutin significantly ameliorated diabetes-induced body weight loss and improved metabolic dysfunctions of hyperglycemic rats. Based on appearance and histopathological staining, rutin promotes wound healing and inhibits production of inflammatory cells. The immunoblotting data indicated that rutin promotes production of antioxidant enzymes induced by nuclear factor erythroid 2-related factor 2 (NRF2), inhibits the expression of matrix metalloproteinases (MMPs) regulated by NF-κB, and decreases the expression of vascular endothelial growth factor (VEGF). It also promotes the expression of neurogenic-related protein (UCH-L1). The aforementioned results indicated that rutin reduces oxidative stress and inflammatory response in hyperglycemic rats, promoting wound healing and subsequently reducing the risk of wound ulcers.

In silico analysis of the potential mechanism of a preventive Chinese medicine formula on coronavirus disease 2019

“…Numerous compounds such as flavonoids, organic acids, iridoids, triterpenoids and volatile components have been reported from this plant ( Song et al, 2014 ). Rutin is a flavonoids compound and has been proved antioxidant and anti-inflammatory effects through promoting production of antioxidant enzyme and reducing the expression of proinflammatory cytokine such as NF-κB ( Chen et al, 2020 ). CGA is an organic acids compound and as a quality control index component of Jin Yin Hua.…”

Potential Effects of Phenolic Compounds That Can Be Found in Olive Oil on Wound Healing

“…In an in vivo model of tissue regeneration, the administration of rutin-supplemented hydrogels reduced the wound area in rats, attributed to a decrease in oxidative stress markers (e.g., lipid peroxidation); the production of carbonyl protein through the oxidation of certain amino-acids, including lysine, proline, or arginine; and/or a treatment-induced increase in protein levels [ 26 ]. In another rat study, the intraperitoneal administration of rutin proved effective in the treatment of diabetic ulcers, reducing the wound area and increasing the percentage closure by stimulating the production of antioxidant enzymes and inhibiting the expression of metalloproteinases and vascular endothelial growth factor (VEGF), thereby lowering oxidative stress and inflammatory responses [ 27 ]. In an in vitro study, Pivec et al [ 28 ] demonstrated that the water-based enzymatic polymerization of rutin enhances its antioxidant activity, promoting the viability of fibroblasts and HaCat cells (non-tumoral human keratinocytes), evidencing its potential usefulness in tissue regeneration and wound healing.…”

Chemical Composition, Antioxidant and Cytoprotective Potentials of Carica papaya Leaf Extracts: A Comparison of Supercritical Fluid and Conventional Extraction Methods

“…Through HPLC analysis, rutin (a flavonol glycoside) was found to be one of the major flavonoids in CPFD and CPEE, in accordance with the literature [ 20 , 43 ]. Rutin is well known for its wound-healing and antioxidant properties, which may partly explain the reason why CPFD and CPEE enhanced fibroblast proliferation after post-treatment with H 2 O 2 [ 44 , 45 ]. Rutin has been proven to enhance the production and accumulation of extracellular matrices during the fibroblast healing process [ 44 ].…”