Effects of rutin on the oxidative stress, proliferation and osteogenic differentiation of periodontal ligament stem cells in LPS-induced inflammatory environment and the underlying mechanism

Zhao, Bin; Zhang, Wenjing; Xiong, Yixuan; Zhang, Yunpeng; Zhang, Dongjiao; Xu, Xin

doi:10.1007/s10735-020-09866-9

Cited by 26 publications

(20 citation statements)

References 40 publications

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“…1 B). According to a report [ 26 , 27 ], hPDLSCs were identified with markers, such as CD90. Flow cytometry showed that CD90 and CD105 were highly expressed, while CD45 and CD34 were not expressed in the cells ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Histone demethylase KDM5A regulates the functions of human periodontal ligament stem cells during periodontitis via the miR-495-3p/HOXC8 axis

Niu

Yan

2022

Regenerative Therapy

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Section: Resultsmentioning

confidence: 99%

Histone demethylase KDM5A regulates the functions of human periodontal ligament stem cells during periodontitis via the miR-495-3p/HOXC8 axis

Niu

Yan

2022

Regenerative Therapy

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“…Reactive oxygen species, mainly produced by mitochondria, have been considered to be key promoters of cell apoptosis (Zhao et al, 2020). Mitochondrial dysfunction leads to excessive ROS accumulation, and ROS can initiate signalling cascades, such as caspase-3 and p53 signalling, that are involved in the signal transduction of apoptosis (Charlier et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

“…Recent studies have shown that leptin can induce reactive oxygen species (ROS)-dependent apoptosis, whereby excessive ROS is an important contributor to the signal transduction of cell apoptosis (Chen et al, 2019;He et al, 2018). Reactive oxygen species are considered to be key promoters of cell apoptosis, and excessive ROS are reported to induce cell apoptosis in hPDLC dysfunction (Zhao et al, 2020). Although leptin has been detected in periodontal cells (Li et al, 2014), the apoptotic effect of leptin in hPDLCs remains unclear.…”

Section: Introductionmentioning

confidence: 99%

Leptin attenuates hypoxia‐induced apoptosis in human periodontal ligament cells via the reactive oxygen species–hypoxia‐inducible factor‐1α pathway

Gao

Zhu

Zhao

et al. 2021

Experimental Physiology

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Hypoxia-induced apoptosis of human periodontal ligament cells (hPDLCs) is an important contributor to the progression of various periodontal diseases. Although leptin has been shown to protect connective tissue cells against hypoxia-induced injury, whether it might do so by attenuating hypoxia-induced apoptosis in hPDLCs remains unclear. Here, using CoCl 2 treatment, we simulated hypoxic conditions in hPDLCs and explored whether apoptosis and reactive oxygen species (ROS) levels were related to hypoxia. After small interfering RNA (siRNA) inhibition of leptin and hypoxia-inducible factor-1α (HIF-1α), the levels of apoptosis, ROS and leptin expression were measured.We showed that in CoCl 2 -treated hPDLCs, significantly higher cell apoptosis rates and ROS accumulation were observed. Cobalt chloride also increased leptin and HIF-1α expression in hPDLCs. Further investigation of the pathway demonstrated that inhibition of ROS attenuated hypoxia-induced cell apoptosis and leptin expression, whereas siRNA inhibition of leptin aggravated hypoxia-induced cell apoptosis and ROS accumulation. Hypoxia induces cell apoptosis and leptin expression in hPDLCs through the induction of ROS and HIF-1α pathways, and leptin shows feedback inhibition on ROS-mediated apoptosis in hPDLCs. These findings suggest a new application of leptin for hypoxic damage in periodontal diseases.

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“…Red mineralized nodules could be observed under the microscope (TS100, Nikon). The nodules were fully dissolved in 2% cetylpyridinium chloride (Sigma-Aldrich), and the absorbance at 562 nm was measured on the microplate reader to detect the relative content of calcified nodules (Zhao et al, 2020).…”

Section: Ars Staining and Analysismentioning

confidence: 99%

MiR-143-3p Inhibits Osteogenic Differentiation of Human Periodontal Ligament Cells by Targeting KLF5 and Inactivating the Wnt/β-Catenin Pathway

Wangzhou

Lai

et al. 2021

Front. Physiol.

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Human periodontal ligament cells (hPDLCs) play a vital role in cell regeneration and tissue repair with multi-directional differentiation potential. microRNAs (miRs) are implicated in the osteogenesis of hPDLCs. This study explored the mechanism of miR-143-3p in osteogenesis of hPDLCs. Osteogenic differentiation of isolated hPDLCs was induced. KLF5 expression during osteogenic differentiation of hPDLCs was detected and then silenced in hPDLCs. Binding relationship between KLF5 and miR-143-3p was predicted and verified. hPDLCs were treated with miR-143-3p mimic or overexpressing KLF5, and then osteogenic specific markers and mineralized nodules were measured. The key factors of the Wnt/β-catenin pathway during osteogenesis of hPDLCs were measured. KLF5 expression was upregulated during osteogenesis of hPDLCs. KLF5 silencing or miR-143-3p mimic reduced osteogenic specific markers and mineralized nodules. Overexpression of KLF5 could reverse the inhibitory effect of miR-143-3p on osteogenic differentiation. miR-143-3p mimic and KLF5 silencing inactivated the Wnt/β-catenin pathway. Activation of the Wnt/β-catenin pathway reversed the repression effect of miR-143-3p mimic on osteogenesis of hPDLCs. In conclusion, miR-143-3p inhibited osteogenic differentiation of hPDLCs by targeting KLF5 and inactivating the Wnt/β-catenin pathway.

show abstract

Effects of rutin on the oxidative stress, proliferation and osteogenic differentiation of periodontal ligament stem cells in LPS-induced inflammatory environment and the underlying mechanism

Cited by 26 publications

References 40 publications

Histone demethylase KDM5A regulates the functions of human periodontal ligament stem cells during periodontitis via the miR-495-3p/HOXC8 axis

Histone demethylase KDM5A regulates the functions of human periodontal ligament stem cells during periodontitis via the miR-495-3p/HOXC8 axis

Leptin attenuates hypoxia‐induced apoptosis in human periodontal ligament cells via the reactive oxygen species–hypoxia‐inducible factor‐1α pathway

MiR-143-3p Inhibits Osteogenic Differentiation of Human Periodontal Ligament Cells by Targeting KLF5 and Inactivating the Wnt/β-Catenin Pathway

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