Effects of running exercise during recovery from hindlimb unloading on soleus muscle fibers and their spinal motoneurons in rats

Ishihara, Akihiko; Kawano, Fuminori; Ishioka, Noriaki; Oishi, Hirotaka; Higashibata, Akira; Shimazu, Toru; Ohira, Yoshinobu

doi:10.1016/j.neures.2003.10.013

Cited by 44 publications

(42 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The soleus muscle is basically an antigravity and posture muscle that is persistently active; therefore, the fiber sizes are more dependent on muscle activity and loading levels. In fact, hindlimb unloading has been previously found to induce remarkable atrophy of fibers in the soleus muscle of rats 26,27) . We observed that the CP group did not have muscle activity and remained still in their cages all day long, except to eat, probably because of their excess body weight.…”

Section: Discussionmentioning

confidence: 98%

Skeletal Muscle Characteristics of Rats with Obesity, Diabetes, Hypertension, and Hyperlipidemia

Nagatomo

Fujino

et al. 2009

JAT

Self Cite

View full text Add to dashboard Cite

Aim: Data on the skeletal muscle characteristics of patients and animals with lifestyle-related diseases are limited. We investigated mRNA expression levels and fiber profiles in the skeletal muscles of rats with obesity, diabetes, hypertension, and/or hyperlipidemia. Methods: The mRNA expression levels of peroxisome proliferator-activated receptors (PPAR and PPAR / ), PPAR coactivator-1 (PGC-1 ), stearoyl-CoA desaturase-1 (SCD-1), carnitine palmitoyl-transferase (CPT ), medium-chain acyl-CoA dehydrogenase (MCAD), and mitochondrial transcriptional factor A (TFAM) in the soleus muscles were compared among 15-week-old control (WR), type 2 diabetic (GK), hypertensive (SHR), and hyperlipidemic (CP) rats. The fiber profiles in the soleus muscles of these rats were also determined. Results: GK rats showed lower PPAR / , PGC-1 , and MCAD expression levels than WR rats. SHR rats showed higher PPAR and MCAD and lower PPAR / expression levels than WR rats. CP rats showed lower PPAR / and higher SCD-1 expression levels than WR rats. The muscles of WR, SHR, and CP rats had low-oxidative type and high-oxidative type A and type C fibers, whereas the muscle of GK rats had only low-oxidative type fibers. Conclusions:The skeletal muscles of rats with lifestyle-related diseases have unique mRNA expression patterns and fiber profiles depending on the type of disease. For example, the lower PGC-1 and MCAD mRNA expression levels in the soleus muscles of type 2 diabetic rats are associated with the presence of only low-oxidative type fibers in the muscle. J Atheroscler Thromb, 2009; 16:576-585.Key words; Lifestyle-related disease, mRNA expression level, Muscle fiber type bility of ATPase at acidic pH. Type A fibers become ATPase negative at a faster rate than type B fibers when the acidity of the preincubation medium increases. Type C fibers have been identified in skeletal muscles, particularly in the soleus muscle of rats; these fibers are ATPase positive, irrespective of the pH of the preincubation medium. Glycolytic enzyme activity is higher in type A, type B, and type C fibers than in type fibers, whereas oxidative enzyme activity is higher in type , type A, and type C fibers than in type B fibers; therefore, the presence of different types of fibers correlates with the metabolic properties of individual skeletal muscles. Skeletal muscles exhibit plasticity and undergo fiber type transitions under various conditions, such as growth, aging,

show abstract

Section: Discussionmentioning

confidence: 98%

Skeletal Muscle Characteristics of Rats with Obesity, Diabetes, Hypertension, and Hyperlipidemia

Nagatomo

Fujino

et al. 2009

JAT

Self Cite

View full text Add to dashboard Cite

show abstract

“…Tonic muscles like soleus present a greater hypotrophy. That is the reason why devising resources to help people have their optimal movements back is extremely important besides the existing electrical stimulation and physical exercises [17][18][19][20][21][22] . Physical exercise is beneficial to muscle recovery as stated by Malysz et al 23 in his study on muscle recovery of Table 2.…”

Section: Discussionmentioning

confidence: 99%

Avaliação de parâmetros histomorfométricos em sóleos de ratos submetidos à remobilização por salto em meio aquático

Silva

Meireles

Nascimento

et al. 2013

Rev Bras Med Esporte

View full text Add to dashboard Cite

show abstract

“…Ishihara et al (21) mostraram os efeitos da falta de carga em membros posteriores e a recuperação com corrida sobre propriedades metabó-licas e morfológicas das fibras musculares do sóleo e motoneurônios de ratos. Os resultados indicaram que a corrida é benéfica para a recuperação da atrofia e diminuição na percentagem de fibras tipo I, além da diminuição da atividade de enzimas oxidativas, induzida pela falta de carga; não havia alterações nas propriedades metabólicas e morfológicas de motoneurônios, que inervavam os sóleos, após diminuição ou aumento da atividade neuromuscular.…”

Section: Discussionunclassified

Efeitos da corrida em esteira em músculos sóleos de ratos encurtados por imobilização

Natali

Silva

Ciena

et al. 2008

Rev Bras Med Esporte

View full text Add to dashboard Cite

O objetivo deste trabalho foi verificar as adaptações de peso e comprimento do músculo sóleo de ratos Wistar machos, além da estimativa do total de sarcômeros em série e comprimento médio dos sarcômeros, quando submetidos a um processo de remobilização em esteira. Foram utilizados 18 ratos (Wistar), divididos em três grupos: GC -músculo sóleo esquerdo (MSE) imobilizado e remobilizado solto; G10 -MSE imobilizado e remobilizado em velocidade de 10m/min; e G12 (n = 6) -MSE imobilizado e remobilizado em velocidade de 12m/min. Os resultados mostraram as seguintes variações, peso muscular: GC -22,35% (p = 0,0089), G10 -12,52% (p = 0,0623), G12 -12,07%, (p = 0,0004); comprimento muscular: GC -5,47% (p = 0,0120), G10 -3,31% (p = 0,2868), G12 0,41% (p = 0,8987); estimativa de sarcômeros em série: GC -15,42% (p = 0,0047), G10 -10,87% (p = 0,0193), G12 -4,97 (p = 0,2409); comprimento de sarcômeros GC 11,16% (p = 0,0142), G10 9,31% (p = 0,1270), G12 5,58% (p = 0,1327). Conclui-se que G12 obteve maior eficácia após o período de imobilização, pois apresentou maior semelhança com o membro não imobilizado.Palavras-chave: reabilitação, músculo esquelético, sarcômeros. ABSTRACTThe aim of this work was to compare weight and length adaptations of the soleus muscle of male Wistar rats as well as estimation of the total number of serial sarcomere and mean sarcomere length, when they are submitted to remobilization on treadmill. 18 Wistar male rats were used and divided in the three following groups: CG -left soleus (LS) muscle immobilized and remobilized free in the cage; G10 -LS muscle immobilized and remobilized in speed of 10 m/min on treadmill; and G12 -LS muscle immobilized and remobilized in speed of 12 m/min. The right muscles (RS) of each animal were used for comparison. The results showed the following variations, muscular weigh: CG -22.35% (p = 0.0089), G10 -12.52% (p = 0.0623), G12 -12.07%, (p = 0.0004); muscle length: CG -5.47% (p = 0.0120), G10 -3.31% (p = 0.2868), G12 0.41% (p = 0.8987); estimation of number of serial sarcomere: CG -15.42% (p = 0.0047), G10 -10.87% (p = 0.0193), G12 -4.97 (p = 0.2409); sarcomere length: CG 11.16%, (p = 0.0142), G10 9.31% (p = 0.1270), G12 5.58% (p = 0.1327). It was concluded that G12 presented the best effectiveness after immobilization period since it presented greater similarity with the non-immobilized soleus.Keywords: rehabilitation, skeletal muscle, sarcomere. INTRODUÇÃOA literatura científica comprova que os músculos esqueléticos possuem plasticidade muscular, ou seja, respondem a diferentes estímulos impostos, dentre eles: exercícios físicos, alterações hormonais, nutrição, denervação, eletroestimulação, imobilização, dentre outros (1)(2)(3)(4) . A inatividade causa ao músculo significativo remodelamento, incluindo a perda de proteínas miofibrilares, mudanças em atividades metabólicas, alterações vasculares e neurais (5) . Contudo, a imobilização, que gera desuso, ainda é uma opção de tratamento freqüentemente usada em distúrbios musculoesqueléticos, apesar da ocorrência da atro...

show abstract

Effects of running exercise during recovery from hindlimb unloading on soleus muscle fibers and their spinal motoneurons in rats

Cited by 44 publications

References 28 publications

Skeletal Muscle Characteristics of Rats with Obesity, Diabetes, Hypertension, and Hyperlipidemia

Skeletal Muscle Characteristics of Rats with Obesity, Diabetes, Hypertension, and Hyperlipidemia

Avaliação de parâmetros histomorfométricos em sóleos de ratos submetidos à remobilização por salto em meio aquático

Efeitos da corrida em esteira em músculos sóleos de ratos encurtados por imobilização

Contact Info

Product

Resources

About