Effects of rosmarinic acid on an experimental model of painful diabetic neuropathy in rats

Abstract

Context: Diabetic neuropathic (DN) pain is one of the diabetes complications. Rosmarinic acid (RA), a natural phenol antioxidant, shows some biological activities, including anti-inflammatory, analgesic, and anti-diabetic effects. Objectives: We investigated the efficacy of RA administration (10 and 30 mg/kg) on streptozotocin (STZ)-induced neuropathy in rats. Material and methods: The animals received saline or RA (10 and 30 mg/kg, p.o.; once daily) for 8 weeks. DN was evaluated by the tail flick (TF) method, formalin test, and tactile allodynia. At the end, all rats were weighed and underwent plasma glucose measurement. Results: There was an increase in licking time during both formalin test phases in diabetic animals (138.5 ± 10.7 and 448.7 ± 2.6 s) that was decreased by RA10 mg/kg (103.5 ± 7.5 and 284.4 ± 19 s) and RA 30 mg/kg (81.8 ± 11 and 192.7 ± 14 s). RA 30 mg/kg caused anti-nociception during the early phase in treated controls (52.1 ± 6 s) than untreated controls (99.4 ± 5.9 s). The TF latency in diabetics (2.9 ± 0.1 s) was increased in RA10 and 30 mg/kg treated diabetics (5.3 ± 0.4 and 6 ± 0.86 s). The paw withdrawal threshold (PWT) of the diabetics (3.6 ± 0.7 g) was increased after RA 10 and 30 mg/kg (13.8 ± 0.3 and 14 ± 0.4 g) treatment. RA did not induce a significant change in body weight and plasma glucose of rats. Conclusion: RA showed efficacy in amelioration of some aspects of DN. Therefore, RA makes a good candidate for DN treatment in clinical studies.

Dietary polyphenols as antidiabetic agents: Advances and opportunities

“…Rosmarinic acid could protect pancreatic β‐cell dysfunction and glucolipotoxicity‐mediated oxidative stress (Govindaraj & Sorimuthu, 2015), ameliorate scopolamine‐induced memory impairment (Hasanein & Mahtaj, 2015) and diabetic neuropathy (Hasanein & Mohammad, 2014), attenuate vascular dysfunction (Sotnikova et al., 2013), and prevent diabetes‐induced sexual disorders (Khaki, Imani, & Golzar, 2012).…”

Antidiabetic Effects of Simple Phenolic Acids: A Comprehensive Review

“…In a similar study, HFD–STZ‐induced diabetic rats with RA (100 mg/kg BW) for 30 days displayed increased insulin sensitivity index, while the levels of blood glucose, HbA1c, advanced glycation end products, TNF‐α, IL‐1β, IL‐6, p38, mitogen‐activated protein kinase, and nuclear factor‐κB were significantly reduced in diabetic rats (Govindaraj and Sorimuthu Pillai, ). Moreover, RA as well as food rich in this compound could be beneficial in the amelioration of diabetic neuropathy in rats (Hasanein and Mohammad, ).…”

daptation of the in vitro Culture of Origanum majorana L. For Production of Phenolic Acids

“…Rosmarinic acid (RA) has various pharmacological activities such as antimutagenic [10], cardio protective [11], antimicrobial [12], antiviral [13], analgesic and anti-inflammatory activities [14]. In addition to its effect on diabetic neuropathy [15]. Chlorogenic acid (CA) has antifungal [16], neuro-protective [17], cell immune responses [18] and hepatic glucose output effects [19].…”