Effects of resveratrol on the growth and enzyme production of Stenotrophomonas maltophilia: a burn wound pathogen

Abstract

Objective: The purpose of this study was to identify the potential of resveratrol in inhibiting the growth and production of two enzymes, hyaluronidase and protease, in Stenotrophomonas maltophilia, which has become a burn wound pathogen of great significance. Method: Stenotrophomonas maltophilia (ATCC 17666) was cultured in nutrient broth and the microbial load was standardised to 0.5 McFarland standard at 600nm. The study included antimicrobial assays (well diffusion and resazurin dye binding method), hyaluronidase expression regulation assay (hyaluronic acid hydrolysis assay and turbidity assay) and protease expression regulation assay (casein hydrolysis assay and determination of specific activity of protease using tyrosine standard). Results: The minimum inhibitory concentration (MIC) of resveratrol against Stenotrophomonas maltophilia was found to be 125µg/ml. Hyaluronidase production in the organism treated with resveratrol was found to be half that in the untreated organism. The specific activity of protease produced by the organism treated with resveratrol was found to be one-quarter that in the untreated organism, as analysed by the tyrosine standard estimation protocol. Conclusion: Resveratrol was found to be a potent compound to treat Stenotrophomonas maltophilia infections. In addition to the antimicrobial and enzyme-regulatory properties of resveratrol, it also shows anti-oxidant and anti-inflammatory properties. This finding has great scope clinically as resveratrol may prove to be an ideal drug to treat burn wound infections.

Calocybe indica extract inhibits the growth and enzyme production of microbes that impair the healing of burn wounds

“…The blank tube contained all reagents except the tyrosine. The absorbance of the reaction tubes was measured colorimetrically at 660 nm (Bharadwaj and Vaidyanathan, 2020).…”

The protective role of resveratrol in diabetic wound healing

“…Infection is commonly observed in diabetic wounds, and its occurrence is related to many factors, such as local hyperglycemia and neuropathy, pathogen invasion, blood circulation disorder, severe inflammation, oxidative stress, local compression, trauma, etc. Stenotrophomonas maltophilia has become a frequently isolated bacterium in burn wounds and it has developed multidrug resistance (Bharadwaj & Vaidyanathan, 2020). Sraddha S et al (Bharadwaj & Vaidyanathan, 2020) resulted that resveratrol (1, 2, 3 and 5 mg/mL) could have an antimicrobial activity against stenotrophomonas maltophilia and they also indicated that resveratrol could reduce the levels of hyaluronidase, as well as protease, which were produced by stenotrophomonas maltophilia .…”

“…Stenotrophomonas maltophilia has become a frequently isolated bacterium in burn wounds and it has developed multidrug resistance (Bharadwaj & Vaidyanathan, 2020). Sraddha S et al (Bharadwaj & Vaidyanathan, 2020) resulted that resveratrol (1, 2, 3 and 5 mg/mL) could have an antimicrobial activity against stenotrophomonas maltophilia and they also indicated that resveratrol could reduce the levels of hyaluronidase, as well as protease, which were produced by stenotrophomonas maltophilia . Alexei B et al (Shevelev et al, 2020) resulted that resveratrol could greatly suppress the reproductions of such bacteria or fungi as Staphylococcus aureus , Pseudomonas aeruginosa and Candida albicans , which are common pathogens that always adhered in wounds and blocked the healing process of wounds; furthermore, the developed tissue engineering biomaterials also promote the availability and efficiency of resveratrol to fight against infection.…”

Emerging Effects of Resveratrol on Wound Healing: A Comprehensive Review

“…Compared to the latter two, RSV treatment resulted in a significant increase in the proportion of macrophages and fibroblasts, which became important in accelerating wound healing and restoring the normal stratification of the epidermis and hair follicles [ 67 ]. In addition, RSV had been found to be an effective compound against Stenotrophomonas in burn wounds [ 68 ]. It is thus clear that RSV could be a candidate for antimicrobial therapy.…”