Effects of resistive exercise and stretching on the soleus muscle of ovariectomized rats

Ywazaki, Julye Leiko; Loureiro, Ana Paula Cunha; Zotz, Talita Gianello Gnoato; Nascimento, Luana Ribeiro; Nobre, Douglas Vizzu; Gomes, Anna Raquel Silveira

doi:10.1590/1809-2950/14720923012016

Cited by 3 publications

(4 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An increase has been reported in CSA when ovariectomized and hysterectomized rats underwent 10 minutes of stretching, twice a week, during 6 weeks [24]. Therefore, it might be supposed that the augment in CSA of the OHS found in the current study might not be explained for muscle damage or swelling but it can indicate hypertrophy as previously reported [24]. However, the cellular and molecular mechanisms by which stretching exercise exerts on skeletal muscle estrogen-depleted must be well investigated.…”

Section: Discussionsupporting

confidence: 62%

“…In this study the association of estrogen depletion and stretching was determinant to induce an increase The effects of stretching on muscle morphometry of ovariectomized rats 7 in CSA which can suggest some inflammation process such as damage of muscle structural proteins and/or muscle fiber swelling and/or hypertrophy [24,25]. As stated previously, estrogen has generally been shown to inhibit inflammation related to leucocyte infiltration into skeletal muscle and accentuate factors related to muscle repair after unaccustomed exercise [25,26].…”

Section: Discussionsupporting

confidence: 55%

“…As stated previously, estrogen has generally been shown to inhibit inflammation related to leucocyte infiltration into skeletal muscle and accentuate factors related to muscle repair after unaccustomed exercise [25,26]. An increase has been reported in CSA when ovariectomized and hysterectomized rats underwent 10 minutes of stretching, twice a week, during 6 weeks [24]. Therefore, it might be supposed that the augment in CSA of the OHS found in the current study might not be explained for muscle damage or swelling but it can indicate hypertrophy as previously reported [24].…”

Section: Discussionmentioning

confidence: 81%

See 2 more Smart Citations

The effects of stretching on muscle morphometry of ovariectomized rats

et al. 2020

Self Cite

View full text Add to dashboard Cite

Efeitos do alongamento na morfometria do músculo em ratas ovarectomizadasLos efectos del ejercicio de estiramiento en la morfometria muscular de las ratas ovariectomizadas Abstract Introduction: Ageing is responsible for structural alterations, declining of all physiological variables, including range of motion and skeletal muscle function, known as sarcopenia. Objective: The aim of the study was to evaluate the effects of stretching on muscle morphometry in ovariectomized rats. Method: 21 female Wistar rats (12 weeks, 218 ± 22 g) were divided into 4 groups: control (CONTROL, n = 3) intact; ovariectomized and hysterectomized (OH, n = 6); Stretching (STRET, n = 6); ovariectomized and hysterectomized and stretching (OHS, n = 6). The rats were subjected to ovariectomy and hysterectomy. The stretching protocol of the soleus muscle lasted 10 repetitions of 1 minute with 45s interval between each repetition performed 3 times a week for 3 weeks. After 3 weeks, the rats were weighed and the muscles of both hind limbs were removed weighed and analyzed at muscle length; serial sarcomere Vidal R, Volkweis G, Ywazaki JL, Randi MAF, Loureiro APC, Gomes ARS. 2 number; sarcomere length; muscle fiber cross-sectional area (MFCSA) and percentage of connective tissue. Results: The final body weight increased in all groups. The serial sarcomere number of STRET was greater than the OH. The muscle fibers' cross-sectional area of OHS was higher than CONTROL. Conclusion: It can be concluded that ovariectomy and hysterectomy prevented sarcomerogenesis even when stretching was applied. However, the stretching protocol enhanced muscle trophismof ovariectomized and hysterectomized rats. It might be suggested that longitudinal growth (serial sarcomeres) and radial (ASTFM) are differently regulated by stretching in intact and/or estrogen depleted (ovariectomy and hysterectomy) skeletal muscle.Introdução: O envelhecimento é responsável por alterações estruturais, com declínio de todas as variáveis fisiológicas, incluindo amplitude de movimento e função muscular esquelética. Objetivo: O objetivo do estudo foi avaliar os efeitos do alongamento na morfometria muscular de ratas ovariectomizadas. Método: Assim, 21 ratas Wistar (12 semanas, 218 ± 22 g) foram divididas em 4 grupos: Controle (CONTROL, n = 3) intacto; Ovariectomizadas e histerectomizadas (OH, n = 6); Alongamento (ALONG, n = 6); Ovariectomizadas e histerectomizadas e Alongamento (OHA, n = 6). As ratas foram submetidas a ovariectomia e histerectomia. O alongamento do músculo sóleo foi composto de 10 repetições de 1 minuto com intervalo de 45s entre cada repetição, realizado 3 vezes por semana, durante 3 semanas. Após 3 semanas, as ratas foram pesadas e os músculos sóleos de ambas as patas foram retirados, pesados e analisados: comprimento do músculo; número de sarcômeros em série; comprimento do sarcômero; área da seção transversal das fibras musculares (ASTFM) e porcentagem de tecido conjuntivo. Resultados: O peso corporal final aumentou em todos os grupos. O número de sarcômeros em sér...

show abstract

Section: Discussionsupporting

confidence: 62%

Section: Discussionsupporting

confidence: 55%

Section: Discussionmentioning

confidence: 81%

See 1 more Smart Citation

The effects of stretching on muscle morphometry of ovariectomized rats

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…Studies with young male rats showed that stretching exercise induced structural changes that increased muscle volume, muscle fiber cross-sectional area, and serial sarcomere number (Coutinho et al, 2004;Cação-Benedini et al, 2014). Ywazaki et al (2016) using an aged rat model verified an increase in muscle fiber cross-sectional area and sarcomerogenesis after a six-week chronic stretching protocol.…”

Section: Introductionmentioning

confidence: 83%

Effects of Chronic Stretching on Soleus Muscle of Wistar Female Rats

et al. 2020

Self Cite

View full text Add to dashboard Cite

The purpose of this study was to evaluate the chronic effects of stretching exercise on soleus muscle histomorphology and histomorphometry of young and aged rats. Thirty-eight female rats were divided into young control group (YCG, n=10;274±50 g); young stretching group (YSG, n=8;274±12 g); aged control group (ACG, n=10;335±39 g); and aged stretching group (ASG, n=10;321±32g). A mechanical apparatus was used to stretch muscle in 4 repetitions, 60 s each, 30 s interval between repetitions in each session, 3 times a week for 3 weeks. Twenty-four hours after the last stretching session, soleus muscle was removed for micromorphology and immunostaining analysis. Data analyses were performed with one-way ANOVA, post-hoc Tukey, or Kruskal-Wallis tests for parametric and nonparametric, respectively (p≤0.05). Muscle fiber cross-sectional area (MFCSA) of ACG was lower (18 %) compared to the YCG. Stretching increased MFCSA comparing YSG to YCG (5,681.15± 1,943.61 µm 2 vs 5,119.84±1,857.73 µm 2 , p=0.00), but decreased comparing ASG to ACG (3,919.54± 1,694.65 µm 2 vs 4,172.82±1,446.08 µm 2 , p=0.00). More serial sarcomere numbers were found in the YSG than YCG (12,062.91±1,564.68 vs 10,070.39±1,072.38, p=0.03). Collagen I and collagen III were higher in YSG than ASG (7.44±7.18 % vs 0.07±0.09 %, p=0.04) and (14.37 %± 9.54 % vs 5.51 %±5.52 %, p=0.00), respectively. TNF-a was greater in ASG than YSG (43.42 %±40.19 % vs 1.72 ± 2.02 %, p=0.00). Epimysium was larger in the YSG compared to YCG (201.83±132.07 % vs 181.09±147.04 %, p=0.00). After 3-week stretching the soleus muscles from aged rats were smaller than their younger counter-parts. Interestingly, while stretching appeared to positively affect young soleus muscle, the opposite was detected in the muscle of the aged rats.

show abstract