Effects of repetitive sevoflurane anaesthesia on immune response, select biochemical parameters and organ histology in mice

Abstract

SummaryAnimal and technical models often require repeated anaesthetic administrations for surgical procedures. As there is evidence for immunomodulatory effects of anaesthesia, the effects of repeated exposure to sevo urane anaesthesia on the immune response in mice were studied. Sevo urane was administered in vivo under conditions that simulate those in clinical procedures. Adult male mice were anaesthetized with 3% sevo urane in oxygen for 40 min weekly for 3 weeks. Untreated animals served as controls. After sevo urane anaesthesia, peripheral blood leukocyte counts, the composition and in vitro function of spleen cells (lymphocytes and macrophages) and the in vivo immune response to a conventional T-dependent antigen were assessed. In addition, liver, spleen and kidney histopathology and also hepatic and renal function were studied. Three days after the latest anaesthetic procedure, the absolute number of both leukocyte and lymphocyte counts were reduced in peripheral blood. Splenic cell composition (LB, LTCD3‡ , LTCD4 ‡ and LTCD8 ‡ ), macrophage function and the mitogen-induced lymphoprolipherative response were preserved. Yet, the in vivo humoral response to a conventional antigen was augmented following the antigenic challenge. Assessment at day 9 after the last anaesthetic procedure revealed the persistence of the humoral response alteration. Nevertheless, sevo urane-treated animals showed no evidence of histological changes or alteration in hepatic or renal function.

Detection of Anti-HPV11-L1 Antibodies in Immune Sera from Patients Suffering from Recurrent Respiratory Papillomatosis Using ELISA

“…It has been shown that general anesthesia has immunmodulatory effects, including changes in natural killer cell activity and T-lymphocyte subpopulations, and augmented antibody secretion in mice (9,21). Specific humoral responses can become altered [i.e., increased as …”

“…described by Elena et al (9)] after antigen administration and anesthesia; however, the latter cannot by itself influence the production of specific polyclonal antibodies. It has also been proposed that genes coding for HLA II may play a role in JORRP development.…”

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“…Here, we review the effects of some of the most common anaesthetic drugs (Table 1) on the immune system. Although some studies have showed effects of anaesthetic drugs on immune response even several days after their administration 9,13,14 , we will focus mainly on the effects within 24 hours after procedure, describing their effects on cells and cytokines of the innate and acquired immune systems.…”

“…Thus, part of the effect of anaesthetics on the immune system would be due to its action on the well-known immunomodulatory effect of glucocorticoids 3 . Diverse in vitro experiments with human immune cells 4 , ex vivo [5][6][7] , in vivo [8][9][10] or animal models have been used to study the effect of anaesthetic drugs on the immune system. .…”

The effect of repeated sevoflurane and nitrous oxide exposure on immunity in rabbits

“…It has been demonstrated that anesthetic agents given during any procedure may lead to immunosuppression (KELBEL and WEISS, 2001). Most clinical studies have been focused on the combined effects of surgery and anesthesia on the immune function (MORISAKI et al, 1998, DELOGU et al, 2000SCHNEEMILCH and BANK, 2001;HORI et al, 2003;COCELLI et al, 2012), with only a few studies dealing specifically with anesthetic agents (DAGAN et al, 1989;MOUDGIL and SINGAL, 1997;PUIG et al, 2002;ELENA et al, 2003;MÜLLER-EDENBORN et al, 2015).…”